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Multichannel microdroplet detection chip for nucleic acid detection

A detection chip, multi-channel technology, applied in specific-purpose bioreactors/fermenters, enzymology/microbiology devices, and microbial determination/inspection, etc. Detection throughput requirements, long detection time and other problems, to achieve the effect of preventing nucleic acid cross-contamination, simple structure, and reducing processing difficulty

Active Publication Date: 2017-12-26
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing digital PCR technology mostly uses flow detection technology, which has high requirements for instruments and a long detection time, which cannot meet the needs of rapid on-site detection of nucleic acids
The existing integrated droplet detection chip can only realize the detection of a single sample, which cannot meet the demand for sample detection throughput

Method used

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  • Multichannel microdroplet detection chip for nucleic acid detection
  • Multichannel microdroplet detection chip for nucleic acid detection
  • Multichannel microdroplet detection chip for nucleic acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 , figure 2 and Figure 4 As shown, a multi-channel droplet detection chip for nucleic acid detection, which is sequentially provided with a cover layer 1, a channel layer 2 and a substrate layer 3 from top to bottom; the material of the substrate layer 3 is selected from glass, polydimethyl One of siloxane, polymethyl methacrylate, polycarbonate, polytetrafluoroethylene, heat-resistant scotch tape, polyethylene terephthalate film; the material of the channel layer 2 is polydimethyl One of siloxane, polymethyl methacrylate, polycarbonate, glass or polytetrafluoroethylene; the material of the cover layer 1 is selected from one of glass, polymethyl methacrylate or polycarbonate. The lamination method of the substrate layer 3 , the channel layer 2 and the cover sheet layer 1 is one of thermal bonding, heat-resistant scotch tape bonding, heat-resistant glue bonding or oxygen plasma treatment.

[0040] Wherein, several independent detection units 2a are a...

Embodiment 2

[0051] Such as figure 1 , image 3 and Figure 4 As shown, a multi-channel droplet detection chip for nucleic acid detection, which is sequentially provided with a cover sheet layer 1, a channel layer 2 and a substrate layer 3 from top to bottom;

[0052] Wherein, several independent detection units 2a are arranged in the channel layer 2;

[0053] Each detection unit 2a includes a dispersed phase hole 201, a sample injection hole 202 and a reaction chamber 203;

[0054] After the dispersed phase hole 201 and the sample injection hole 202 converge through the first microchannel 205 and the second microchannel 206 respectively, they are connected to the reaction chamber 203 through the third microchannel 207;

[0055] A reaction chamber main hole 204b is also provided in the channel layer 2, and the reaction chamber main hole 204b communicates with the reaction chamber 203 of each detection unit 2a through the fifth microchannel 208b.

[0056] Wherein, the two sides of the mul...

Embodiment 3

[0059] The application of the multi-channel droplet detection chip in embodiment 1 or embodiment 2 in digital nucleic acid detection can be applied to droplet preparation, nucleic acid amplification and detection, specifically through the following steps:

[0060] (1) Mix the target DNA molecule with the isothermal amplification reaction reagent, add it to the sample injection hole 202, and add the fluorinated oil containing the fluorinated surfactant to the dispersed phase hole 201;

[0061] (2) When the pressure driving system is a vacuum negative pressure system, the vacuum negative pressure system is sealed and connected to the reaction chamber hole 204a (or the reaction chamber main hole 204b), and vacuumed to make the vacuum degree reach 5-50kPa. After the vacuum degree is stable, open the solenoid valve; (or when the pressure drive system is a syringe pump positive pressure system, connect the syringe pump positive pressure system to the sample injection hole 202 and the...

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Abstract

The invention discloses a multichannel microdroplet detection chip for nucleic acid detection, which is successively provided with a cover plate layer, a channel layer and a substrate layer from top to bottom, wherein the channel layer is internally provided with a plurality of independent detecting units; each detecting unit comprises a dispersion phase hole, a sample adding hole, a reaction cavity and a reaction cavity hole; the dispersion phase holes and the sample adding holes are respectively converged through a first micro-channel and a second micro-channel, then are communicated to the reaction cavity through a third micro-channel; the other end, opposite to the third micro-channel, in the reaction cavity is communicated with the reaction cavity hole through a forth micro-channel. According to the multichannel microdroplet detection chip, a chip structure of a plurality of parallel detecting units is adopted to improve the reaction flux; a buckle is used for connecting different chips, the different chips can be detected in a combining manner, the flux is flexible and adjustable, and the detection efficiency is improved; the multichannel microdroplet detection chip is simple in structure, the application cost is greatly reduced compared with the present digital PCR instrument, and can be applied to the primary clinical detection.

Description

technical field [0001] The invention relates to a droplet detection chip, in particular to a multi-channel droplet detection chip for nucleic acid detection. Background technique [0002] Current biomedical research is going deep into the molecular level from the whole and cellular level. Nucleic acid is an important class of biomolecules in cells, which participates in the regulation of most functions of cells, such as gene expression and silencing, organelle composition, and cell behavior regulation. Understanding the content and distribution of nucleic acids in different cells is crucial to the in-depth study of their functions and the biological significance behind them, and is of great significance to the diagnosis and treatment of malignant tumors, AIDS, and genetic diseases. [0003] Existing nucleic acid detection mainly adopts polymerase chain reaction (PCR), which has high sensitivity and good specificity, and is currently the most commonly used genetic diagnosis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
CPCC12Q1/6844C12Q2565/629C12Q2563/159
Inventor 黎海文张涛周武平刘聪蒋克明印晨宇
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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