Preparation method and application of scallop edge whitening peptide

A scallop edge and whitening technology, which is applied to the preparation method of peptides, chemical instruments and methods, skin care preparations, etc., can solve the problem of increasing ovarian cell chromosome exchange and chromosome aberration, light, heat and metal ion instability, affecting The whitening effect and appearance of the product are solved, and the effect of easy operation, simple preparation method and high safety is achieved

Active Publication Date: 2019-10-11
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Commonly used chemical whitening agents such as arbutin, vitamin C and its derivatives, and kojic acid in China have good whitening effects, but there are some defects in the formula: for example, kojic acid can inhibit the activity of tyrosinase Active, but it is unstable to light, heat and metal ions, easy to change color, and long-term use will have cytotoxicity, which will increase the probability of ovarian cell chromosome exchange and chromosome aberration; although arbutin is safe, it basically has no stimulation Sexual and allergic, but high concentrations can depigment normal skin
In short, the defects of this type of chemical whitening agent have greatly affected the whitening effect and appearance of the product.

Method used

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  • Preparation method and application of scallop edge whitening peptide
  • Preparation method and application of scallop edge whitening peptide
  • Preparation method and application of scallop edge whitening peptide

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preparation example Construction

[0026] In view of this, in a typical embodiment of the present invention, a preparation method of scallop edge whitening peptide is provided. The preparation method includes the following steps:

[0027] (1) Pretreatment of scallop edges: take the scallop edges, cut them into pieces, and homogenize the scallop edges with water at a multiple of the quality of the scallop edges to obtain a scallop edge homogenate;

[0028] (2) Enzymatic hydrolysis: adding pepsin and trypsin to the scallop rim homogenate in step (1) for enzymatic hydrolysis to obtain an enzymatic hydrolysate;

[0029] (3) Adjust the pH of the enzymatic hydrolysate in step (2) to 6.8~7.2, centrifuge to collect the supernatant for concentration, add the set proportion of absolute ethanol, stir and alcohol precipitation, after centrifugation, the supernatant is the crude scallop rim polypeptide Extract; After purification, scallop edge whitening peptide is obtained;

[0030] In step (1), specifically, the scallop side is ri...

Embodiment 1

[0041] (1) Pretreatment of scallop edges: 2kg of fresh scallops are rinsed with tap water to remove the internal organs, sediment and scallop pillars. Take the scallop edges, rinse the scallop edges with tap water and double distilled water, cut into pieces, and soak 0.5 in pure water. h, Weigh in addition to water. Add 8 times the amount of pure water and disperse with a homogenizer to obtain a scallop edge homogenate.

[0042] (2) Double enzymatic hydrolysis: heat the scallop rim homogenate in a 50℃ water bath, adjust the pH of the homogenate to 2.0 with 6mol / L HCl, add pepsin to 0.4% of the scallop rim, adjust the pH to 2.3, and use a constant temperature water bath at 50℃ After enzymatic hydrolysis for 5 hours, stirring constantly, the pH of the whole process is between 2.0 and 2.5. Heat the enzymatic hydrolysis solution in a water bath at 50°C, continue to adjust the pH to 8.2 with 0.5mol / L NaOH, add trypsin, the weight is 0.45% of the scallop side, adjust the pH to 8.2, en...

Embodiment 2

[0045] (1) Pretreatment of scallop edges: 2kg of fresh scallops should be rinsed with tap water to remove the internal organs, sediment and scallop stems, take the scallop edges, rinse the scallop edges with tap water and double distilled water, cut into pieces, and soak in pure water for 1h , Weighing in addition to water. Add 6 times the amount of pure water and disperse with a homogenizer to obtain a scallop edge homogenate.

[0046] (2) Double enzymatic hydrolysis: heat the scallop rim homogenate in a 50℃ water bath, adjust the pH of the homogenate to 2.0 with 6mol / L HCl, add pepsin to 0.5% of the scallop rim, adjust the pH to 2.3, and use a constant temperature water bath at 50℃ After enzymatic hydrolysis for 5 hours, stirring constantly, the pH of the whole process is between 2.0 and 2.5. Heat the enzymolysis solution in a water bath at 50°C, continue to adjust the pH to 8.0 with 0.5mol / L NaOH, add trypsin, the weight is 0.45% of the scallop side, adjust the pH to 8.2, enz...

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Abstract

The invention discloses a preparation method and application of a scallop edge whitening peptide. The preparation method of the whitening peptide includes: (1) scallop edge pretreatment: take the scallop edge, cut it into pieces, add water with a set multiple of the scallop edge mass to carry out Homogenize to get scallop edge homogenate; (2) Enzymolysis: Add pepsin and trypsin to the scallop edge homogenate in step (1) successively for enzymolysis to obtain enzymolysis; (3) Adjustment step (2) The pH of the enzymolysis solution is 6.8-7.2, centrifuged to take the supernatant to concentrate, add a set proportion of absolute ethanol, stir alcohol precipitation, the supernatant after centrifugation is the crude extract of scallop edge polypeptide; after purification, the scallop Edge Whitening Peptides. The invention only needs simple double enzymatic hydrolysis and alcohol precipitation process to obtain the scallop edge whitening peptide which can significantly inhibit the activity of tyrosinase, has the application potential as an active ingredient of whitening cosmetics, and has high activity, good biocompatibility, and safety Advantages such as non-toxic and side effects.

Description

Technical field [0001] The invention belongs to the technical field of cosmetics, and specifically relates to a preparation method and application of a scallop edge whitening peptide. Background technique [0002] With the continuous growth of people's consumer demand, the cosmetics industry has developed rapidly. As the so-called "one white covering three ugliness", smooth, fair and ruddy skin has always been pursued by oriental women, so whitening and moisturizing has gradually become a consistent aesthetic standard. The main substance that affects skin color is melanin. Melanin is a biological pigment, which is formed by a series of chemical reactions of tyrosine or 3,4-dihydroxyphenylalanine, which is produced by melanocytes and stored in it. Melanocytes in melanocytes have a special enzyme called tyrosinase, which can oxidize tyrosine into dopa, then oxidize into dopaquinone, and finally produce melanin. Melanin moves through the layers of cell metabolism and reaches the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/30A61K8/64A61Q19/02
Inventor 宋淑亮王宇张娥吉爱国
Owner SHANDONG UNIV
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