Composite artificial cerebral dura mater and preparation method thereof

An artificial dura mater and composite technology, applied in the fields of filament/thread forming, medical science, textiles and papermaking, etc., to achieve the effects of favorable cell growth, small film pores, good mechanical properties and flexibility

Inactive Publication Date: 2018-01-05
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of PVDF for dura mater repair materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] In this embodiment, the composite artificial dura mater is obtained by electrospinning with polyvinylidene fluoride (PVDF) as the main support material and the degradable polymer PLGA as the adjustment material of the oriented fibers.

[0049] The preparation method comprises the following steps:

[0050] (1) Dissolve PVDF with a molecular weight of 275,000 in pure DMF solvent, heat and stir at 50° C. for 24 hours to obtain a PVDF material solution with a concentration of 20% (w / v%).

[0051] (2) Dissolve PLGA with a molecular weight of 60,000 (fragment ratio 50:50) in pure DMF solvent, heat and stir at 25°C for 24 hours to obtain a PLGA solution with a concentration of 20% (w / v%).

[0052] (3) The PVDF material solution obtained in step (1) and the PLGA solution obtained in step (2) are ejected from two nozzles of the electrospinning device respectively, and the temperature is 25° C. during the electrospinning, and the relative humidity is 50%. The receiver is a squir...

Embodiment 2

[0054] In this embodiment, the composite artificial dura mater is obtained by electrospinning with polyvinylidene fluoride (PVDF) as the main support material and the degradable polymer PLCL as the adjustment material of the oriented fibers.

[0055] The preparation method comprises the following steps:

[0056] (1) Dissolve PVDF with a molecular weight of 275,000 in DMF / acetone (volume ratio 1:1) mixed solvent, heat and stir at 50°C for 24 hours to obtain a PVDF material solution with a concentration of 25% (w / v%).

[0057] (2) Dissolve PLCL with a molecular weight of 60,000 (fragment ratio 50:50) in a mixed solvent of DMF / acetone (volume ratio 1:1), heat and stir at 25°C for 24 hours to obtain a concentration of 25% (w / v%) PLCL solution.

[0058] (3) The PVDF material solution obtained in step (1) and the PLCL solution obtained in step (2) are ejected from two nozzles of the electrospinning device respectively, and the temperature is 25° C. during the electrospinning, and t...

Embodiment 3

[0060] In this embodiment, the composite artificial dura mater is obtained by electrospinning with polyvinylidene fluoride (PVDF) as the main support material and the degradable polymer PGCL as the adjustment material of the oriented fibers.

[0061] The preparation method comprises the following steps:

[0062] (1) Dissolve PVDF with a molecular weight of 400,000 in DMF / acetone (volume ratio 1:1) mixed solvent, heat and stir at 40°C for 36 hours to obtain a PVDF material solution with a concentration of 15% (w / v%).

[0063] (2) Dissolve PGCL with a molecular weight of 40,000 (fragment ratio 25:75) in a mixed solvent of DMF / acetone (volume ratio 1:1), heat and stir at 40°C for 10 hours to obtain a concentration of 20% (w / v%) PGCL solution.

[0064] (3) The PVDF material solution obtained in step (1) and the PGCL solution obtained in step (2) are ejected from two nozzles of the electrospinning device respectively, and the temperature is 30° C. during the electrospinning, and t...

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PUM

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Abstract

The invention provides a composite artificial cerebral dura mater and a preparation method thereof. The composite artificial cerebral dura mater is made of polyvinylidene fluoride (PVDF) as a main body support material and a degradable polymer as an adjusting material of an orientation fiber through electrostatic spinning. The composite artificial cerebral dura mater provided by the invention is of a structure similar to an extracellular scaffold, is beneficial to cell migration, proliferation and differentiation; the main body support material PVDF in the composite material is good in proliferation and is beneficial to cell growth around a wound, a film is small in aperture, cerebrospinal fluid leakage or infection caused by bacteria is prevented, meanwhile good mechanical properties andflexibility are achieved, the composite material is good in biocompatibility, can be degraded in vivo, simple in preparation method, low in material cost and beneficial to industrial production, and degradation products are free of biotoxicity.

Description

technical field [0001] The invention relates to the field of biomedical materials, and relates to a composite artificial dura mater and a preparation method thereof. Background technique [0002] The dura mater is a layer of connective tissue located on the inner side of the skull and the outer side of the brain. It is a natural barrier to protect brain tissue and is of great significance for maintaining the structure and functional activities of the nervous system. The natural dura mater is mainly composed of fibroblasts and collagen cells. Congenital defects, trauma or surgery can cause dural defects, which need to be repaired in time to avoid cerebrospinal fluid leakage, causing encephalocele and intracranial infection. [0003] An ideal material for dura mater repair should meet the following conditions: ①It has stable biological inertia and does not cause acute and chronic inflammatory reactions. ② It is safe, non-toxic, non-carcinogenic, and non-teratogenic. It is re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/58D01D5/00D04H1/4382D04H1/728D04H1/76
Inventor 刘子凡许杉杉韩志超
Owner SHENZHEN UNIV
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