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Biosensor for detecting myeloperoxidase based on copper-palladium-platinum nano mesh materials

A biosensor and myeloperoxidase technology, which is applied in the field of electrochemical detection, can solve the problems of no application in the field of electrochemistry, and achieve the effects of promoting development, facilitating commercialization, and increasing the solid loading capacity.

Inactive Publication Date: 2018-01-05
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, copper-palladium-platinum nanowire mesh materials have not been used in the field of electrochemistry

Method used

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  • Biosensor for detecting myeloperoxidase based on copper-palladium-platinum nano mesh materials
  • Biosensor for detecting myeloperoxidase based on copper-palladium-platinum nano mesh materials
  • Biosensor for detecting myeloperoxidase based on copper-palladium-platinum nano mesh materials

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Experimental program
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Effect test

Embodiment 1

[0032] Step 1. Add 6.00 mg of NaBH 4 dissolved in 3mL ultrapure water, then 2mL containing 1.94mg Na 2 PdCl 4 , 3.32 mg CuCl 2 and 93.32mL H 2 PtCl 6 (5%) mixed solution is added to above-mentioned NaBH rapidly under magnetic stirring (300rpm) 4 in solution. React at room temperature for 2 minutes. Afterwards, the reaction solution was quickly transferred to an EP tube (2 mL), and the solid product was collected by centrifugation (10000 rpm), washed three times with ultrapure water, and finally placed in a vacuum oven to dry for use.

[0033] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0034] Step 3. Add 10 μL of the electrode modification material copper palladium platinum nanowire mesh material (CuPdPt NNWs) dropwise on the ...

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Abstract

The invention discloses a novel unmarked electrochemical immunosensor for detecting myeloperoxidase (MPO) based on copper-palladium-platinum nano wire mesh materials (CuPdPt NNWs). Due to the fact that a copper-palladium-platinum nano wire mesh structure has excellent electronic conductivity and shows good catalytic activity on hydrogen peroxide, the copper-palladium-platinum nano wire mesh materials are regarded as novel effective electric catalyst. Meanwhile, the mesh structure provides a large surface area for electro-deposition gold nanoparticles (AuNP). The AuPNs not only improves conduction of electrons, but also fixes antibodies through covalent binding. As a result, the unmarked immunosensor shows more sensitive detection on the MPO. Under the best experimental condition, the immunosensor shows a wide linearity range from 100 fg mL1 to 50 ng mL1, and the lowest detectable limit is 33.3 fg mL-1(S / N=3). In addition, the unmarked immunosensor is used for measuring the MPO in humanserum, and the recovery rate is 99.8-103.6%, so that it is indicated that the electrochemical immunosensor can be applied to actual detection of the MPO. Meanwhile, the electrochemical immunosensor can also be used for detection of other biomarkers in the serum.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical immunosensor for quantitatively detecting myeloperoxidase in clinical practice, especially to construct an immunosensor based on a copper-palladium-platinum nanowire mesh composite material as a signal by a direct method for detection Myeloperoxidase belongs to the field of electrochemical detection. Background technique: [0002] Coronary heart disease is the most common disease in the cardiovascular system, and acute coronary syndrome (ACS) is a severe coronary heart disease. Due to the high morbidity and poor prognosis of ACS, early diagnosis of ACS is very important. Myeloperoxidase (MPO) is an abundant phagocyte blood protein in mammals. Biochemical and genetic studies have shown that the content of MPO in the blood is closely related to the prevalence of cardiovascular diseases. Increased MPO levels in blood have been shown to predict the risk of cardiovascula...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N27/327
Inventor 于超文艺霖袁建勇
Owner CHONGQING MEDICAL UNIVERSITY
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