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Preparation method of pollen pini active peptide

A technology of pine pollen and active peptides, which is applied in the direction of allergic diseases, non-central analgesics, hydrolyzed protein components, etc., can solve the problems of high utilization rate of the body, waste of raw materials, absorption and utilization rate of the human body, etc., and achieve food safety improvement, Good taste and immune-boosting effect

Inactive Publication Date: 2018-01-12
赵德润
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the waste of raw materials in the extraction process of pine pollen active substances in the prior art and the shortcomings of low absorption and utilization by the human body, the invention provides a preparation method of pine pollen active peptides, which are rich in biologically active peptides, It has pharmacological activities such as anti-arteriosclerosis, lowering blood fat and cholesterol, analgesia, anti-radiation, prevention and treatment of diabetes, enhancement of immunity, anti-cancer and anti-cancer, etc., and is easy to digest and absorb, rich in nutrients and high utilization rate of the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, 1. Preparation of pine pollen active peptide liquid: select 20 kg of fresh dried pine pollen in the same year for ultrafine pulverization; add water 10 times the weight of superfine pulverized pine pollen, dilute and soak for 30 minutes, stir evenly, break and homogenate After adjusting the PH value of pine pollen to 6, add cellulase, edible fungus enzyme, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the enzyme dosage is 9500 enzymes per gram of pine pollen Live unit (u), temperature controlled at 50 degrees Celsius, enzymatic hydrolysis for 4.5 hours; after enzymolysis, boiling water heat bath to inactivate the enzyme for 10 minutes, cooling and centrifuging, the centrifuge speed is about 9000rpm, and the pine pollen active peptide liquid is obtained;

[0015] 2. Put the pine pollen active peptide solution prepared above into a stainless steel barrel and put it into a steam sterilizer, and sterilize it by steam and high pressure at 100 ...

Embodiment 2 1

[0017] Embodiment 2. 1. Preparation of pine pollen active peptide liquid: select 25 kg of fresh dried pine pollen of the year and carry out ultrafine pulverization treatment; add water 9 times the weight of ultrafine pulverized pine pollen to dilute and soak for 30 minutes, stir evenly, break and homogenate The final pine pollen is adjusted to PH value 4, and cellulase, edible fungus enzyme, neutral protease, papain (1:1:1:2) are added for enzymatic hydrolysis, and the amount of enzyme added is 10,000 per gram of Ganoderma lucidum spore powder Enzyme activity unit (u), temperature control 55 degrees Celsius, enzymolysis for 6 hours; after enzymolysis, boiling water heat bath to inactivate enzyme for 15 minutes, cooling and centrifuging, centrifuge speed is about 8000rpm, and pine pollen active peptide liquid is obtained.

[0018] 2. Put the pine pollen active peptide solution prepared above into a stainless steel barrel and put it into a steam sterilizer for 30 minutes of steam...

Embodiment 3 1

[0020] Embodiment three, 1, the preparation of pine pollen active peptide liquid: select the fresh dry clean pine pollen 15kg of that year and carry out superfine pulverization treatment; add the water of 15 times of superfine pulverization pine pollen weight and carry out diluting and soaking for 30 minutes and stir evenly, break up evenly Adjust the pH value of the pulped pine pollen to 4.5, add cellulase, edible fungus enzyme, neutral protease, papain (1:1:1:2) for enzymatic hydrolysis, and the enzyme dosage is 8000 per gram of pine pollen Enzyme activity unit (u), temperature controlled at 58 degrees Celsius, enzymolysis for 4.5 hours; after enzymolysis, boiling water heat bath to inactivate the enzyme for 12 minutes, cooling and centrifugation, the centrifuge speed is about 6000rpm, and the pine pollen active peptide liquid is obtained.

[0021] 2. Put the pine pollen active peptide solution prepared above into a stainless steel barrel and put it into a steam sterilizer fo...

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Abstract

The invention discloses a preparation method of pollen pini active peptide, and relates to the fields of traditional Chinese medicines and biotechnology. The pollen pini active peptide, with fresh anddried pollen pini serving as a raw material, is prepared by conducting superfine grinding, homogenizing, enzymatic hydrolysis, centrifuging and sterilizing. Pollen pini active peptide liquid is prepared by regulating pH value of the pollen pini, which is obtained after conducting superfine grinding and homogenizing, to 3-6, conducting enzymatic hydrolysis with the addition of a complex enzyme ofcellulase, edible fungus enzyme, neutral protease and papain (1:1:1:2) by 5000-10000 enzyme-activity unit ([mu]) to each gram of the pollen pini at a temperature of 50-60 DEG C for 3-6h; and then, conducting enzyme inactivating, centrifuging and sterilizing. Freeze-dried powder products, oral liquid products and capsule products can be prepared. The pollen pini has pharmacological activities of resisting arteriosclerosis, reducing blood lipid and cholesterol, alleviating pain, resisting radiation, preventing and treating diabetics, boosting immunity, preventing and resisting cancers and the like. The preparation method provided by the invention is scientific and rigorous in process, strong in operability and easy for industrial production.

Description

technical field [0001] The invention relates to the fields of traditional Chinese medicine and biotechnology. In particular, a pine pollen active peptide prepared from pine pollen and a preparation method thereof. Background technique [0002] Pine pollen is the pollen of Chinese native tree species - Pinus massoniana and Pinus tabulaeformis growing in mountainous areas at an altitude of 1100-1500 meters. Compared with bee-sourced pollen, pine pollen is collected artificially. It has a single flower source, pure quality, stable ingredients, and no Pesticide residues, no animal hormones and other characteristics. Pine pollen tastes better than any other plant pollen, and it has a light fragrance when taken. The ancients said: "The air of pines and cypresses can make people live longer!" So pine pollen is called "the king of pollen". It has been proved by scientific research and practice that long-term use has no toxic and side effects. Pine pollen is the sperm cell of the...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P9/10A61P3/06A61P29/00A61P39/00A61P3/10A61P37/04A61P35/00
Inventor 赵德润
Owner 赵德润
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