Gene detection probe and gene detection method

Abstract

The invention discloses a gene detection probe and a gene detection method. The gene detection probe comprises inactivated nuclease protein, a marking molecule and guide RNA, and the marking moleculeand the guide RNA are connected to the inactivated nuclease protein which is inactivated Cas9 protein or inactivated Cpf1 protein. The gene detection method includes: subjecting a to-be-detected geneand the gene detection probe to incubation at the room temperature to obtain a gene-probe complex, and performing signal detection on the obtained gene-probe complex to realize gene detection. The gene detection probe can be hybridized with the to-be-detected gene at the normal temperature and is high in to-be-detected gene affinity and recognition specificity, high in hybridization speed and highin sensitivity, and quickness and accuracy in gene detection are realized.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis, and specifically relates to a gene detection probe and a gene detection method. Background technique [0002] Accurate, sensitive, and specific analysis of biological functional molecules (such as nucleic acids and proteins) is of great scientific significance in the fields of life science and biomedical research and clinical diagnosis of diseases. Conventional biochemical analysis and medical diagnosis have experienced the development of landmark technologies such as radiology, radioimmunology, and immuno-optics. In recent years, they have gradually transitioned to the category of molecular diagnostics. In the 21st century, thanks to multidisciplinary synthesis, nucleic acid probes and sensing technologies have achieved deeper and broader development. For example, through the development of immune-PCR (Immune-PCR) and aptamer probe technology, nucleic acid probes have been able to ...

AI Technical Summary

Problems solved by technology

Although the detection method based on nucleic acid probe hybridization has become a gold standard for gene analysis, it still faces the following problems: 1) The hybridization of nucleic acid probes requires a temperature shift process, for example, heat denaturation of gene amplification products is usually required After the nucleic acid is denatured, it becomes two single strands, and then the detection probe is specifically hybridized with the product, and then annealed to return to room temperature. Because the denaturation step requires a temperature controller, the detection step is complicated; 2) conventional nucleic acid Hybridization methods usually require high concentrations of hybridization probes, because usually high concentrations of probes need to be added so that the thermodynamic reaction equilibrium favors the direction of hybridization rather than the direction of annealing. In addition, even with high concentrations of probes, the hybridization The efficiency is also often very low, and this is because the length of the amplification product is often greater than the length of the probe, thereby causing a competition between the renaturation and hybridization of the nucleic acid; 3) conventional hybridization methods are usually slow, often taking more than an hour, Hybridization of target genes at low concentrations often requires overnight reactions

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