Method of detecting trivalent chromium in health food
A health food and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not considering the conversion of hexavalent chromium to trivalent chromium, inaccurate detection results, etc.
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Embodiment 1
[0139] Weigh Cr (Ⅲ) single element standard stock solution (GBW08614, 1000mg / L, National Standard Material Research Center), with nitric acid-EDTA disodium buffer solution (pH value is 7.1, 50mmol / L nitric acid+0.6mmol / L EDTA) to prepare samples to be tested with concentrations of 0, 3, 9, 18, 50, and 100 μg / L, filter through a 0.22 μm organic filter membrane, and perform liquid chromatography and inductively coupled plasma mass spectrometry analysis;
[0140] The chromatographic condition of described liquid chromatography is:
[0141] Analytical column: cation exchange column;
[0142] Mobile phase: nitric acid-EDTA disodium buffer solution, the pH value of the nitric acid-EDTA disodium buffer solution is 7.1;
[0143] Flow rate: 0.5mL / min;
[0144] Elution method: isocratic elution;
[0145] Injection volume: 10μL;
[0146] Detection time: 2min;
[0147] The conditions for inductively coupled plasma mass spectrometry are:
[0148] Atomizer: glass concentric atomizer;...
Embodiment 2
[0159] Take 0.0503 g of chromium picolinate tablets and pulverize them, add 2 mL of 10% HCl solution and 0.1 mol / L hydrochloric acid into the Erlenmeyer flask, sonicate at 80°C for 30 min, cool to room temperature, and under the condition of pH 7.1 Complexation reaction with mL nitric acid-ethylenediaminetetraacetic disodium buffer solution (pH value 7.1, 50mmol / L nitric acid + 0.6mmol / L EDTA) in a 50°C water bath for 1h, after cooling to room temperature, Disodium amine tetraacetate buffer solution (pH value is 7.1, 50mmol / L nitric acid + 0.6mmol / L EDTA) was diluted to the concentration range of the standard curve, and finally filtered through a 0.22μm organic filter membrane for liquid chromatography and inductance Coupled plasma-mass spectrometry analysis.
[0160] Using the same liquid chromatography and inductively coupled plasma mass spectrometry conditions as in Example 1 to detect the liquid to be tested, the obtained liquid chromatogram is as follows image 3 as show...
Embodiment 3
[0162] Using the same steps as in Example 2, the only difference is that 0.0503 g of the chromium picolinate tablet is replaced by 0.0510 g. The obtained phase chromatogram is as Figure 4 as shown, Figure 4 Middle 1 is the Cr(Ⅲ) peak, and the area of peak 1 is 829633. The concentration of Cr(Ⅲ) in the health food test solution calculated by the external standard method and the standard equation is 81.704μg / L, and the content of Cr(Ⅲ) in the sample is calculated to be 1602.0mg / kg. The calculation results are based on the repeatability conditions. The arithmetic mean of the results obtained for two independent determinations is represented with a relative standard deviation of 2.0%.
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Abstract
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