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Fungicide applied to organic fertilizer of edible fungus residues and preparation method thereof and application of fungicide in organic fertilizer

An organic fertilizer and edible fungus technology, which is applied in the field of agricultural microorganism application, can solve the problems of poor quick-acting fertilizer effect and low number of probiotics, and achieves the effect of promoting biological characteristics, low price, and improving soil.

Inactive Publication Date: 2018-01-23
山东世纪智慧农业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems that the number of probiotics in the organic fertilizer of the decomposed edible fungus residue is small and the effect of the quick-acting fertilizer is poor, the present invention provides a bacterial agent for the organic fertilizer of the edible fungus waste residue that can increase beneficial bacteria

Method used

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  • Fungicide applied to organic fertilizer of edible fungus residues and preparation method thereof and application of fungicide in organic fertilizer
  • Fungicide applied to organic fertilizer of edible fungus residues and preparation method thereof and application of fungicide in organic fertilizer
  • Fungicide applied to organic fertilizer of edible fungus residues and preparation method thereof and application of fungicide in organic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 bacterial agent.

[0030] 1 Fermentation culture technology of Bacillus subtilis

[0031] 1.1 Test tube slant culture

[0032] The bacteria were activated and cultured on the inclined plane of the test tube, and the cellulose medium with the following components was used: NaCl 0.3g; MgSO 4 ·7H 2 O0.3g; MnSO 4 4H 2 O 0.03g; KCl 0.3g; (NH 4 ) 2 SO 4 8g; FeSO 4 ·7H 2 O 0.03g; KH 2 PO 4 3.0g; sucrose 2g; sodium carboxymethylcellulose 5g; agar 15-20g; distilled water 1000mL; pH 7.0-7.5. Inoculate sterilely and incubate at 30°C for 3 days.

[0033] 1.2 Shake flask culture

[0034] Add 80mL of the liquid cellulose medium without agar in the above 1.1 into a 250mL Erlenmeyer flask, inoculate Bacillus subtilis aseptically after sterilization, and then place the Erlenmeyer flask on a reciprocating shaker at 30°C for 3 days. Frequency 180 rpm.

[0035] 1.3 Seed tank culture

[0036] Add 100L of the liquid cellulose medium that does...

Embodiment 2

[0050] The number of viable bacteria and the determination of enzyme activity in the bacterial agent of embodiment 2.

[0051] 1. Bacteria agent live bacteria count

[0052] The number of viable bacteria in the bacterial agent was counted by the plate colony counting method, and the specific method was as follows:

[0053] (1) Take about 1.0 g of the bacterial agent, accurately weigh the mass, put it into 10 ml of sterile water, shake it up and filter it, and take the filtrate for experiment;

[0054] (2) Accurately take 1ml of filtrate, add 9ml of sterile water, and repeatedly dilute the filtrate 10 times into 8 serial concentrations, numbered 1-7;

[0055] (3) Select three dilutions of bacterial solutions numbered 5-7, take 0.2ml respectively and put them into a sterile plate, then pour about 20ml of medium that has been melted and cooled to about 45°C, and mix with the bacterial solution Mix well, cool down, and wait for solidification to form a flat plate, repeat twice, ...

Embodiment 3

[0068] Fertilizer effect test of embodiment 3 biological organic fertilizer.

[0069] The bacterial agent produced in Example 1 was used to evaluate the application effect of the edible fungus residue and the bacterial agent in the field by the effect test of fertilizing wheat.

[0070] 1 Materials and methods

[0071] 1.1 Test location

[0072] Winter wheat field, Gegezhuang Village, Xiong County, Baoding City, Hebei Province.

[0073] 1.2 Types of soil to be tested

[0074] The area of ​​the test field is 5 mu, the soil type is a sandy mud field developed from river alluvium, and the soil structure is APWB. The soil nutrient content of the plow layer is 27.8g / kg of organic matter, 1.34g / kg of total nitrogen, 115mg / kg of alkaline nitrogen, pH5.5, 49mg / kg of available phosphorus, 85mg / kg of available potassium, and 1.16g / m3 of bulk density 3 , porosity 56.2%.

[0075] 1.3 Test crops

[0076] Xinmai 26, sown on October 3.

[0077] 1.4 Conventional fertilization

[0078]...

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Abstract

The invention discloses a fungicide applied to organic fertilizer of edible fungus residues and a preparation method thereof and application of the fungicide in the organic fertilizer, with an aim tosolve problems that existing composted edible fungus residue organic fertilizer is small in amount of probiotics and poor in quick-acting fertilizer effect. The fungicide capable of increasing the probiotics is composed of, by weight, 1-5% of bacillus subtilis, 1-5% of trichoderma asperellum, 5-10% of an anti-ultraviolet agent and 80-93% of an adsorbent, wherein the additive amount of the fungicide in the edible fungus residues is 4-6kg / t. The fungicide mixed with the bacillus subtilis and the trichoderma asperellum is added in the organic fertilizer of the edible fungus residues, biological nature of the organic fertilizer is promoted, the amount of beneficial microorganisms and the probiotics in the organic fertilizer is increased, and soil improvement function of the organic fertilizeris improved; the fungicide is of plant material like bran, straw meal and turf soil, the raw material is easy to obtain, price is low, pesticide residues like straws can be utilized greatly, and wasteis made profitable.

Description

technical field [0001] The invention relates to a bacterial agent used for edible fungus residue organic fertilizer, a preparation method thereof and an application in edible fungus residue decomposed organic fertilizer, belonging to the application field of agricultural microorganisms. Background technique [0002] The use of organic fertilizers in agricultural production can improve soil physical and chemical properties and increase soil fertility. It is conducive to the formation of a good soil structure, thereby improving the tightness, air permeability, water permeability, water retention and thermal conditions of the soil. It can also promote the activity of soil microorganisms. Organic fertilizer not only contains a large number of elements necessary for plants, medium and trace elements, but also rich in organic nutrients. Organic fertilizer is a comprehensive fertilizer. Moreover, organic fertilizers can be obtained locally and used locally, with wide sources and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F11/08C12N1/20C12N1/14C12R1/125C12R1/885
Inventor 徐计同王波杨爱兰
Owner 山东世纪智慧农业科技有限公司