Method for inducing proliferation and rooting of succulent plants by utilizing processing of light sources different in wave length

A succulent, wavelength technology, applied in the application, fertilizer mixture, flower cultivation and other directions, can solve the problems of slow proliferation, reduced economic benefits, slow rooting, etc., to achieve the effect of accelerating rooting rate, increasing proliferation rate and avoiding pollution.

Inactive Publication Date: 2018-01-30
樊昌华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are certain defects in the tissue culture seedlings of succulents, which are manifested in slow proliferation and slow rooting, which leads to a long time for hardening, transplanting, and marketing of succulents after the tissue culture is completed, and the economic benefits are reduced.
Therefore, many scholars are committed to studying the rapid proliferation and rooting methods of succulents. However, at p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for inducing rapid proliferation and rooting of succulents by treating with light sources of different wavelengths, characterized in that the medium is configured and the culture conditions are set, and the steps are specifically as follows:

[0016] (1) Culture medium configuration

[0017] Described culture medium is conventional MS solid medium, then add calcium chloride dihydrate of 5mg / L, potassium dihydrogen phosphate of 25mg / L, magnesium sulfate heptahydrate of 12mg / L, potassium chloride of 40mg / L , 0.4mg / L boric acid, 0.1mg / L sodium molybdate dihydrate, 3mg / L zinc sulfate heptahydrate, 1mg / L manganese sulfate tetrahydrate, 0.1mg / L copper sulfate pentahydrate, 0.6mg / L L of sodium iron edetate, 0.6mg / L of hormone NAA, 0.4mg / L of hormone 6-BA, 1.6 mg / L of hormone IAA, 1.8mg / L of hormone GA, 14mg / L of downy mildew Hydrochloride, 6mg / L of cresylsulfonic acid, 2mg / L of hymexazol, 4mg / L of urotropine, 6mg / L of vitamin B 1 , 6mg / L vitamin B 2 , 3mg / L vitamin...

Embodiment 2

[0023] (1) Culture medium configuration

[0024] Described culture medium is conventional MS solid medium, then add calcium chloride dihydrate of 7mg / L, potassium dihydrogen phosphate of 28mg / L, magnesium sulfate heptahydrate of 15mg / L, potassium chloride of 52mg / L , 0.5mg / L boric acid, 0.2mg / L sodium molybdate dihydrate, 4.5mg / L zinc sulfate heptahydrate, 1.6mg / L manganese sulfate tetrahydrate, 0.15mg / L copper sulfate pentahydrate, 0.65 mg / L sodium iron EDTA, 0.75 mg / L hormone NAA, 0.5 mg / L hormone 6-BA, 1.6 mg / L hormone IAA, 2.2 mg / L hormone GA, 15 mg / L Propamocarb hydrochloride, 7.5mg / L cresolsulfonic acid, 4mg / L hymexazol, 5mg / L urotropine, 7mg / L vitamin B 1 , 7.5mg / L vitamin B 2 , 4mg / L vitamin B 6 , 5mg / L vitamin B 9 ;

[0025] (2) Culture condition setting

[0026] Take the aseptically treated succulent explants and inoculate them into the culture medium, seal them with a breathable film, and place them in an artificial climate box for cultivation. The temperature...

Embodiment 3

[0030] A method for inducing rapid proliferation and rooting of succulents by treating with light sources of different wavelengths, characterized in that the medium is configured and the culture conditions are set, and the steps are specifically as follows:

[0031] (1) Culture medium configuration

[0032]Described culture medium is conventional MS solid medium, then add calcium chloride dihydrate of 10mg / L, potassium dihydrogen phosphate of 30mg / L, magnesium sulfate heptahydrate of 16mg / L, potassium chloride of 60mg / L , 0.6mg / L boric acid, 0.3mg / L sodium molybdate dihydrate, 5mg / L zinc sulfate heptahydrate, 2mg / L manganese sulfate tetrahydrate, 0.2mg / L copper sulfate pentahydrate, 0.8mg / L L of sodium iron edetate, 0.8 mg / L of hormone NAA, 0.6 mg / L of hormone 6-BA, 1.8 mg / L of hormone IAA, 2.4 mg / L of hormone GA, 16 mg / L of downy mildew Hydrochloride, 8mg / L cresolsulfonic acid, 5mg / L hymexazol, 6mg / L urotropine, 8mg / L vitamin B 1 , 8mg / L vitamin B 2 , 5mg / L vitamin B 6 , ...

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PUM

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Abstract

The invention provides a method for inducing proliferation and rooting of succulent plants by utilizing processing of light sources different in wave length. Trace elements, an antibacterial agent, hormones and vitamins are added in a culture medium, the nutrient demands of succulent plant tissue culture seedlings in the growth process are met, meanwhile the pollution caused in the tissue cultureprocess is avoided, and proliferation and rooting of the succulent plant tissue culture seedlings are promoted by utilizing the hormones and vitamins. Based on the culture medium, the method utilizesthe red, blue and green light sources different in wave length at different growth stages of the succulent plants, red light is utilized to improve the proliferation rate of succulent plant tissue culture seedlings, blue light is utilized to make the seedlings strong, and green light is utilized to promote rooting; the three colors of light are used crossly in a later period to improve the proliferation rate and rooting rate of the succulent plant tissue culture seedlings and make the seedlings strong. In addition, compared with a conventional method, the proliferation rate of the succulent plant tissue culture seedlings is improved by 2.32 times, and the fresh weight of the cultured succulent plant tissue culture seedlings is improved by 20.08%.

Description

technical field [0001] The invention relates to the field of succulent plant cultivation, in particular to a method for inducing the proliferation and rooting of succulent plants by treating light sources with different wavelengths. Background technique [0002] In recent years, the market demand for succulents is extremely high, and the supply is in short supply. Therefore, a large number of succulents in the modern market come from tissue culture seedlings produced by tissue culture. There are certain defects in the tissue culture seedlings of succulents, which are manifested in slow proliferation and slow rooting, which in turn leads to a longer time for hardening, transplanting, and marketing of succulents after the tissue culture is completed, and the economic benefits are reduced. Therefore, many scholars are committed to studying the rapid proliferation and rooting methods of succulents. However, at present, a large amount of research is still focused on the deploymen...

Claims

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Application Information

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IPC IPC(8): A01G22/60C05G3/00
Inventor 樊昌华
Owner 樊昌华
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