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Dioscorea cirrhosa propagation method through tissue culture

A technology for tissue culture and yam, applied in the field of tissue culture and reproduction of yarrow, can solve the problems of scientific research difficulties, inability to guarantee the survival rate, and no tissue culture of yam, achieves high survival rate, is convenient for large-scale popularization of planting, The effect of inhibiting browning

Inactive Publication Date: 2018-02-02
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on Ranunculus yam, and most of them are reports on pharmacological components; in wild growth, terminal bud propagation, side stem propagation and seed propagation are the main propagation methods of Ranunculus yam, but these several reproduction methods in nature The survival rate cannot be guaranteed in the state of the disease, which brings great difficulties to scientific research
The prior art does not have relevant reports on the tissue culture of Buttercup yam

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for tissue culture propagation of Rhizoma dioica, the terminal buds of Ranunculus radiata are taken as explants, and after disinfection treatment, the following cultures are carried out respectively:

[0021] Step 1. Place the explants in the induction medium and cultivate until axillary buds are obtained. The light intensity is 2100lx, the light period is 10h / d, and the temperature is 24°C. The induction medium includes: MS, 5g / L Agar, 30g / L sucrose, 2mg / L total flavonoids and 0.8mg / L gibberellin, adjust the pH value of the induction medium to 5.8;

[0022] Step 2. Place the axillary buds in the proliferation medium for proliferation and culture to obtain clustered buds. The light intensity is 2100lx, the photoperiod is 12h / d, and the temperature is 25°C, wherein the proliferation medium includes: MS, 3.5g / L Agar, 30g / L sucrose, 0.5mg / L total flavonoids of Odontodon grape, 2mg / L TDZ plant growth regulator and 0.2mg / L forchlorfenuron, adjust the pH value of the...

Embodiment 2

[0032] The rooted seedlings of Acanthus osmanthus cultivated in Example 1 were hardened, and the healthy hardened seedlings were selected and transplanted to the substrate, and the substrate was composed of cinnamon extract, perlite, pine scales, and straw fermentation products in a mass ratio of 3:20:2: 10; the stalk fermentation product is produced by mixing and fermenting cotton stalks and corn stalks at a mass ratio of 3:2. Cultivate 50 strains of yam seedlings in embodiment 2, the average root number of 50 strains of yam seedlings is 2.1, and the average root length is 3.22cm. long.

Embodiment 3

[0040] In the present embodiment, the tissue culture method of the explant of Rhizoma dioica is the same as that of Example 1. Before carrying out the tissue culture of the explant, 100 explants have been carried out anti-browning pretreatment, specifically: the explant Put it in the decoction of Xiaoqinglong Decoction, and irradiate it under an infrared lamp for 15 minutes; the preparation method of the decoction of Xiaoqinglong Decoction is: put the decoction of Xiaoqinglong Decoction in a casserole with water, soak it for 2-3 hours, and then boil it with strong fire , turn to low heat and decoct until the water volume in the casserole is 1 / 3 of the volume of the original water volume; among them, soak 5g of Xiaoqinglong soup per liter of water.

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PUM

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Abstract

The invention discloses a dioscorea cirrhosa propagation method through tissue culture. Terminal buds of dioscorea cirrhosa serve as explants, and culture is conducted as follows: the explants are placed in an induction culture medium for culture to obtain axillary buds; the axillary buds are placed in a proliferation culture medium for proliferation culture to obtain cluster buds; the cluster buds are placed in a strong seedling culture medium for strong seedling culture to obtain test-tube seedlings; the test-tube seedlings are placed in a rooting culture medium to obtain rooting seedlings,wherein the rooting culture medium is prepared from 1 / 2 MS, 3.5 g / L of agar, 30 g / L of sucrose, 0.2 mg / L of isoleucine, 0.3 mg / L of Brazilian magnet powder and 0.5 mg / L of traditional Chinese medicineextract, and the traditional Chinese medicine extract is formed by mixing cinnamon leaf extract and oldenlandia diffusa extract with the mass ratio of 1:2. The method has the advantages that the probability of browning of dioscorea cirrhosa in the tissue culture process is inhibited, and the root-growing rate and the survival rate are improved.

Description

technical field [0001] The present invention relates to the field of tissue culture. More specifically, the present invention relates to a tissue culture propagation method of Rhizoma yam. Background technique [0002] Dioscrea cirrhosa Lour., also known as blood mother, red boy, goat's head, boar potato, and golden flower fruit, is a perennial vine wild plant in the genus Dioscrea, and its tubers are used for medicine. It is rich in southern Zhejiang, Fujian, Taiwan, Hunan, southern Jiangxi, Guangdong, Guangxi, Sichuan, Yunnan, and Medog in Tibet. It has a long history of folk application, and its main biologically active ingredient is the tannin in the tuber, which can promote blood circulation and stop bleeding, regulate qi and relieve pain, astringe and astringent, clear away heat and detoxify, and has good curative effect on many diseases. Economic value, wild plants waiting for large-scale development and utilization, suitable for extensive promotion and planting. A...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈乾平谷筱玉白隆华潘丽梅龙海荣蒋妮冯世鑫
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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