Fluorescein-rhodamine B copper ion ultraviolet molecular probe applied to naked eye detection and synthesizing and using methods thereof

Abstract

The invention discloses a fluorescein-rhodamine B copper ion ultraviolet molecular probe applied to naked eye detection and synthesizing and using methods thereof, relates to a Cu<2+> probe and synthesis and application thereof and aims at solving the technical problems that an existing Cu<2+> molecular probe needs to use an expensive-price high-precision fluorescence spectrophotometer for detection and is prone to being interfered by external environment. A structural formula of the molecular probe disclosed by the invention is shown in a following description. According to a preparation method of the molecular probe, the molecular probe is prepared by the steps of utilizing fluorescein mono-aldehyde and rhodamine B hydrazine to react and utilizing a mixed solvent of methylene dichlorideand methyl alcohol to separate through a column chromatography. A use method of the molecular probe comprises the steps of dissolving the probe in a mixed solution of MeOH and HEPES buffer solution, detecting an added sample to be detected and judging that Cu<2+> is contained if color of the solution is changed into deep pink from light pink; or detecting ultraviolet spectrum absorbance values before and after the sample is added and judging that the sample solution contains the Cu<2+> if the absorbance value is improved when the wavelength is 502 and 553nm. A detection method is simple, quickand visual.

Description

technical field [0001] The present invention relates to Cu 2+ Synthesis and application of molecular probes for detection. Background technique [0002] Cu 2+ An essential trace element widely distributed in biological tissues, Cu 2+ Insufficient or overdose can lead to a series of neurological diseases, such as Menkes syndrome, Alzheimer's disease, Wilson's disease and Parkinson's disease. At the same time Cu 2+ It is also one of the common pollutants in industrial environments. According to the report of the World Health Organization (WHO), Cu 2+ The standard content in reference water should be lower than 20μmol / L. Therefore, it can accurately and quantitatively detect Cu in physiological environment and natural environment. 2+ The content is of great significance, the current detection of Cu 2+ Among the many methods, the spectroscopic analysis method has attracted the attention of scholars because of its high selectivity and high sensitivity. The spectroscopic ...

AI Technical Summary

Problems solved by technology

[0004] The present invention will solve existing Cu 2+ The probe needs to be detected by an expensive high-precision fluorescence spectrometer and is easily interfered by the external environment. 2+ Technical problems of molecular probes, and provide a kind of fluorescein-rhodamine B copper ion ultraviolet molecular probes for naked eye detection and its synthesis and use method

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