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Preparation method of active brown-sugar lactobacillus

A lactic acid bacteria, active black technology, applied in bacteria and other directions, can solve problems such as the increase in the number of harmful bacteria, the imbalance of the balance of human colon and digestive system flora, and infection, so as to benefit human health, improve human immunity, Nutritious effect

Inactive Publication Date: 2018-02-16
SUZHOU JIANSHIXING BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, under modern diet, high pressure of life and work, and pathological conditions, the number of harmful flora increases, and neutral flora produces harmful substances to the human body, turning into harmful flora, which leads to the natural balance of human colon and digestive system flora Loss of balance: gas production, abdominal distension, diarrhea and constipation, etc., susceptible to infection by pathogenic bacteria, the human body is in a sub-healthy or pathological state

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of active brown sugar lactic acid bacteria, the steps are:

[0025] (1) Configuration of MRS solid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume; high pressure 1× 10 5 Pa sterilization 20min;

[0026] (2) The configuration of the improved MRS liquid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 10g / L brown sugar, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjusted to pH 6.6-6.8, and constant volume; high pressure 1×10 5 Pa sterilization 20min;

[0027] (3) Activate the JSX-BS2 strain with MRS solid medium at 37°C; ...

Embodiment 2

[0035] (1) Configuration of MRS solid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume; high pressure 1× 10 5 Pa sterilization 20min;

[0036] (2) The configuration of the improved MRS liquid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L brown sugar, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjusted to pH 6.6-6.8, and constant volume; high pressure 1×10 5 Pa sterilization 20min;

[0037] (3) Activate the JSX-BS2 strain with MRS solid medium at 37°C;

[0038] (4) Inoculate the activated JSX-BS2 strain into the improved MRS liquid medium; ...

Embodiment 3

[0045] (1) Configuration of MRS solid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume; high pressure 1× 10 5 Pa sterilization 20min;

[0046] (2) The configuration of the improved MRS liquid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 30g / L brown sugar, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjusted to pH 6.6-6.8, and constant volume; high pressure 1×10 5 Pa sterilization 20min;

[0047] (3) Activate the JSX-BS2 strain with MRS solid medium at 37°C;

[0048] (4) Inoculate the activated JSX-BS2 strain into the improved MRS liquid medium; ...

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Abstract

The invention discloses a preparation method of an active brown-sugar lactobacillus. Firstly, a strain which can quickly grow and propagate in a culture medium prepared from brown sugar is screened from an existing probiotic population, and a fermentation condition is continuously optimized. One lactobacillus strain, i.e. JSX-BS2, which can quickly grow and propagate in a brown-sugar culture medium is screened and obtained from the strain; next, the deep fermentation is carried out on the JSX-BS2 strain by using the brown-sugar culture medium. Afterwards, an embedment protecting agent is addedinto a fermentation product; the deep freeze-drying processing is carried out to form probiotic powder; further, a lactobacillus health product in a different dosage form is produced. The product contains a brown-sugar nutritional component, a lactobacillus and a metabolite of the lactobacillus, is richer, in nutrient, than an original lactobacillus product, can be used for preventing multiple diseases, is used for improving the immunity of a human body, is beneficial to the health of the human body, and is applicable to crowds at various age stages, particularly to a female.

Description

technical field [0001] The invention relates to a preparation method of active brown sugar lactic acid bacteria, in particular to the fermentation of probiotics and the preparation of healthy food, and belongs to the technical field of food fermentation engineering. Background technique [0002] Probiotics are a general term for a class of active microorganisms that can improve the host's micro-ecological balance and produce definite health effects to exert beneficial effects. Probiotics can be roughly divided into three categories, including: Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, etc.); Bifidobacteria (such as Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium ); and Gram-positive cocci (such as Streptococcus faecalis, Lactococcus, etc.). In addition, there are some yeasts that can also be classified as probiotics. Probiotics mainly have the following effects and functions on the human body: they can inhibit the reproduction of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 刘世名
Owner SUZHOU JIANSHIXING BIOLOGICAL SCI & TECH CO LTD