Extracting method for toosendanin

An extraction method and technology for azadirachtin, applied in the directions of steroids, organic chemistry, etc., can solve the problems of effective chemical activity destruction of azadirachtin, many impurities, low yield, etc., and achieve high efficiency absorption, high extraction rate, Simple to use effects

Inactive Publication Date: 2018-02-23
JIANGSU GOLDSUN TEXTILE SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] At present, the extraction methods of azadirachtin are mainly conventional extraction method, ultrasonic extraction method, microwave method, rapid extraction method, supercritical and subcritical solvent method, etc., but the above-mentioned methods all have disadvantages in varying degrees, which are mainly manifested in the extraction In the method, using ethanol as the solvent extraction method has a low yield; the azadirachtin product obtained by the rapid extraction method using an organic solvent contains a variety of oily component residues, and there are many impurities; The effective chemical activity of the hormone produces a destructive effect

Method used

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  • Extracting method for toosendanin

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Effect test

Embodiment 1

[0029] Wash and dry the neem bark, crush it to 100-200 mesh, add water and mix, the mass ratio of neem powder and water is 1:50; after fully mixing, add cellulase and pectinase, cellulase and pectin The mass ratio of enzyme and neem powder is 1:1:20, adjust the mixed system to adjust the pH value to 3.5-5.5, the temperature is 40-50°C, and fully react for 2 hours in an ultrasonic field with an ultrasonic power of 200W and an ultrasonic frequency of 20KHz , Filtrate 1 and filter residue 1 were obtained by filtration.

[0030] Add the filter residue 1 to the disodium hydrogen phosphate-phosphate buffer solution with a pH value of 2.2, mix evenly at room temperature, fully extract the filtrate 2 and filter residue 2 in an ultrasonic field with an ultrasonic power of 200W and an ultrasonic frequency of 20KHz for 2 hours The filter residue 2 is added to the phthalic acid-sodium hydroxide buffer solution with a pH value of 5.5, mixed uniformly at room temperature, and the filtered f...

Embodiment 2

[0033] Wash and dry the neem seeds, crush them to 100-200 mesh, add water and mix, the mass ratio of neem powder and water is 1:80; after mixing well, add cellulase and pectinase, cellulase and pectin The mass ratio of enzyme and neem powder is 1:1:15, adjust the mixed system to adjust the pH value to 3.5-5.5, the temperature is 40-50°C, and fully react for 2 hours in an ultrasonic field with an ultrasonic power of 200W and an ultrasonic frequency of 20KHz , Filtrate 1 and filter residue 1 were obtained by filtration.

[0034] Add the filter residue 1 to the acetic acid-sodium acetate buffer solution with a pH value of 2.6, mix evenly at room temperature, fully extract the filtered filtrate 2 and filter residue 2 in an ultrasonic field with an ultrasonic power of 200W and an ultrasonic frequency of 20KHz for 2 hours; The filter residue 2 was added to the disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 6.0, mixed uniformly at room t...

Embodiment 3

[0037] Wash and dry the neem bark, crush it to 100-200 mesh, add water and mix, the mass ratio of neem powder and water is 1:100; after fully mixing, add cellulase and pectinase, cellulase and pectin The mass ratio of enzyme and neem powder is 1:1:10, adjust the mixed system to adjust the pH value to 3.5-5.5, the temperature is 40-50°C, and fully react for 2 hours in an ultrasonic field with an ultrasonic power of 200W and an ultrasonic frequency of 20KHz , Filtrate 1 and filter residue 1 were obtained by filtration.

[0038]Add the filter residue 1 to the acetic acid-ammonium acetate buffer solution with a pH value of 3.0, mix evenly at room temperature, fully extract the filtered filtrate 2 and filter residue 2 in an ultrasonic field with an ultrasonic power of 200W and an ultrasonic frequency of 20KHz for 2 hours; The filter residue 2 was added to the disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 6.5, mixed uniformly at room t...

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Abstract

The invention provides an extracting method for toosendanin. The method comprises the following steps: firstly, washing and drying melia azedarach bark or melia azedarach seeds, smashing, and extracting by using cellulase and pectinase water solution; extracting filter residues by successively using buffer solution, wherein the pH value of the buffer solution is 2.0-3.0, 5.0-7.0 and 8.0-8.5, and performing the membrane separation to filter liquor after extracting by an organic solvent, reserving the solution, and concentrating in the low temperature to prepare the toosendanin. The extracting method is capable of separating and extracting toosendanin raw materials by creatively using a semi-bionic technology, the obtained toosendanin solution contains effective ingredients which can be highly absorbed by an animal body. A membrane separation reservation technology is combined to obtain a toosendanin product of which the molecular weight is less than or equal to 1000 Da. Under the synergistic effect of the semi-bionic technology and the membrane separation technology, the content of the effective ingredients of the finally obtained toosendanin product is higher.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, in particular to a method for extracting azadirachtin. Background technique [0002] Azadirachtin, also known as azadirachtin, is mainly extracted from the bark and fruit of the neem tree. It has a variety of physiological activities. Good broad-spectrum insecticidal and antibacterial properties, non-destructive to the human body, easy to degrade, can be used to prevent and control crop diseases and insect pests, and is a plant-derived pesticide with great potential. [0003] At present, the extraction methods of azadirachtin are mainly conventional extraction method, ultrasonic extraction method, microwave method, rapid extraction method, supercritical and subcritical solvent method, etc., but the above-mentioned methods all have disadvantages in varying degrees, which are mainly manifested in the extraction In the method, using ethanol as the solvent extracti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
CPCC07J71/0021
Inventor 毛军陈红霞陆鹏袁洪胜
Owner JIANGSU GOLDSUN TEXTILE SCI & TECH
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