Biologically active polypeptide YPQRDMPIQAF and preparation method and application thereof

A technology of biologically active peptides and derivatives, applied in the field of protein, can solve the problems of poor digestibility and absorption, unexplained performance, etc., and achieve the effect of reducing the incidence of the body, enhancing the function, and promoting the increase of the induced amount

Inactive Publication Date: 2018-03-20
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The β-casein-H disclosed in this patent is a macromolecular protein with poor digestibility and absorption
The protein disclosed in this patent is a biological macromolecule and has excellent dietary functions, but it does not specify whether it has other properties

Method used

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  • Biologically active polypeptide YPQRDMPIQAF and preparation method and application thereof
  • Biologically active polypeptide YPQRDMPIQAF and preparation method and application thereof
  • Biologically active polypeptide YPQRDMPIQAF and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The artificial synthesis of embodiment 1 active peptide YPQRDMPIQAF

[0036] 1. Synthesis of Bioactive Peptides

[0037] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak in 50ml of dichloromethane (DCM).

[0038] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0039] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0040] 4. Take a small amount of resin and use ninhydrin (Nine Wells Ninhydrin) method to test it (Two drops each of Test A and Test B, react at 100°C for 1 min), if the resin is colored, it means that the deprotection is successful.

[0041] ...

Embodiment 2

[0086] Anti-inflammatory activity experiment of embodiment 2 bioactive peptides

[0087]1. The experiment of bioactive polypeptide YPQRDMPIQAF promoting macrophages to secrete cytokines (ELISA method)

[0088] 1. Experimental reagents and instruments:

[0089] Reagents: experimental animal balb / c mouse (male 6-8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO Company; Serum albumin (bovine serum albumin, BSA), Genebase Company; milk-derived bioactive polypeptide YPQRDMPIQAF obtained in Example 1; ELISA cytokine rapid kit (TNF-α, IL-1β and IL-6), Wuhan Boster Bioengineering Ltd.

[0090] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus; Dragon WellscanMK3 microplate reader Labsystems.

[0091] 2. Experimental method...

Embodiment 3

[0110] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0111] 1. The experiment of biologically active polypeptide YPQRDMPIQAF improving the viability of Drosophila

[0112] 1. Experimental reagents and instruments:

[0113] Reagent: Oregon K wild-type Drosophila melanogaster, Genetics Laboratory, College of Agriculture, Shanghai Jiaotong University; agar powder, Sinopharm Chemical Reagent Co., Ltd.; milk-derived bioactive polypeptide YPQRDMPIQAF obtained in Example 1.

[0114] Equipment: CM-230 Mole ultra-clean water, Shanghai Mole Scientific Instrument Co., Ltd.; G136T Zealway intelligent high-temperature sterilization pot, Xiamen Zhiwei Instrument Technology Co., Ltd.; BJ-CD SERIES biological incubator, Shanghai Boxun Industrial Company; GRX-9073 hot air disinfection box, Shanghai Yiheng Technology Co., Ltd.

[0115] 2. Experimental method:

[0116] Take Drosophila as the experimental model: collect newly eclosioned Drosophila adults within 8 hours, a...

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Abstract

The present invention relates to the field of proteins, and in particular relates to biologically active polypeptide YPQRDMPIQAF and a preparation method and application thereof, and the amino acid sequence of the biologically active polypeptide YPQRDMPIQAF is Tyr-Pro-Gln-Arg-Asp-Met-Pro-Ile-Gln-Ala-Phe. In-vitro anti-inflammatory activity experiments and in-vivo anti-aging experiments verify thebiologically active polypeptide YPQRDMPIQAF has good anti-inflammatory activity and anti-aging activity, on the one hand, the biologically active polypeptide YPQRDMPIQAF can promote cell factor secretion of macrophages, promotes increase of NO induced amount of the macrophages, improves the external pathogen infection resisting ability of the body, and reduces the incidence of diseases of the body; on the other hand, the biologically active polypeptide YPQRDMPIQAF can enhance in-vivo anti-peroxidase system vitality, enhances the exogenous stimulus-resistant function of the body so as to reducethe probability of aging, senility and illness of the body, and has very important significance for development of food, health care products and drugs with anti-inflammatory and anti-aging functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide YPQRDMPIQAF and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soybean peptides, a...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12A23L33/18A61K38/17A61K8/64A61P39/06A61P29/00A61Q19/08
CPCC07K14/4732A23L33/18A23V2002/00A61K8/64A61K38/00A61Q19/08A23V2250/55A23V2200/302
Inventor 张少辉张伯宇李婉如林学海汪超陈静李云飞范梦珠
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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