Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing freeze-drying bacterial powder of high-activity bifidobacterium and application

A technology of bifidobacteria and bacterial powder, which is applied in the production field of biological bacteria products, can solve problems such as strain differences, and achieve the effect of improving self-stress resistance and freeze-drying survival rate

Inactive Publication Date: 2018-04-03
JIANGNAN UNIV
View PDF7 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there are few specific lyoprotectants, and different lyoprotectants have obvious differences in the survival rate of strains of different species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Freeze-drying of pseudo-small chain bifidobacteria

[0025] (1) Preparation of Pseudomonas bifidobacterium film fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, seven Magnesium sulfate hydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L , Tween-801g / L, soybean powder 10g / L.

[0026] (2) Fermentation preparation of pseudosmall-chain bifidobacterium film: activate bifidobacteria for 3 generations, transfer to a 10L fermenter according to the inoculation ratio of 3-5%, continuously feed nitrogen, adjust the rotation speed, 37 degrees Incubate at constant pH (pH=6) for 24 hours.

[0027] (3) Collection of pseudosmall-chain bifidobacterium pellicles: at 4°C, the bifidobacterium pellicle fermentation broth was centrifuged to collect the bacterium sludge....

Embodiment 2

[0035] The specific implementation method is the same as in Example 1, the difference is that oat bran powder is used to replace soybean powder to carry out bacterial film fermentation, and the freeze-drying results show that the freeze-dried survival rate of bacterial film formed with oat flour as a solid medium is 90%, which is significantly higher The freeze-dried survival rate of bacteria in the non-biofilm state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 63%.

Embodiment 3

[0037] The specific implementation method is the same as that in Example 1, except that the formula of the lyoprotectant is: 80g / L skimmed milk powder, 40g / L stachyose, 40g / L sucrose, 0.5g / L vitamin C, and the pH is 6.5. The freeze-drying survival rate of pseudosmall chain bifidobacteria with biofilm is 87%, which is significantly higher than the freeze-drying survival rate of bacteria in non-bacteria state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 51%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing freeze-drying bacterial powder of high-activity bifidobacterium and application, belonging to the field of producing biological bacterium products. The method comprises the following steps of utilizing strong stress resistance of a bacterial thallus velum state to environmental stress, taking non-water-soluble dietary fiber, which is derived from grain flour, soya flour, vegetable powder, mushroom powder and the like, as a solid phase carrier to ferment and produce bifidobacterium velum thalluses; after fermentation, collecting bacterial sludge tomix with a freeze-drying protective additive, and performing vacuum freeze-drying to prepare the bacterial powder of bifidobacterium. By utilizing the method, the freeze-drying survival rate of the bifidobacterium is between 68% and 93% and is up to 5*1,010cfu / g or more, compared with the bifidobacterium in a non-velum state, the freeze-drying survival rate is improved by 200% or more and the storage stability is improved by 300% or more. By the method, the key common technical problem of developing the bacterial powder of the bifidobacterium with high activity and strong stress resistance issolved, and the prepared bacterial powder of the bifidobacterium can be applied to product forms such as leavening agents and solid beverage.

Description

technical field [0001] The invention relates to a production method and application of high-activity bifidobacteria freeze-dried bacterial powder, belonging to the production field of biological bacteria products. Background technique [0002] Bifidobacterium is one of the necessary probiotics for the human body, and has many beneficial effects on the human body: ①Maintain the balance of human intestinal flora and inhibit the growth of pathogenic bacteria; ②Reduce the cholesterol level in human blood and prevent high blood pressure; ③ Improve the lactose tolerance of dairy products and increase the digestibility of dairy products; ④ enhance the immune function of the human body. [0003] The human body mainly increases the content of bifidobacteria in the body by ingesting live bifidobacterium products. The common live bifidobacteria products on the market include: yogurt, bifidobacterium tablets, bifidobacterium chocolate, etc. Most of these products are made of Made from ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L2/39A23L2/60A23L33/135C12R1/01
CPCA23L2/39A23L2/60A23L33/135C12N1/04C12N1/20
Inventor 陈卫陆文伟翟齐啸林炳渝崔树茂赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com