Stress inducible promoter, stress inducible promoter plant expression vector and expression method of induced target gene
A plant expression vector and target gene technology, applied in the field of stress-inducible promoters, can solve problems such as affecting plant physiological balance, protein overexpression, affecting yield, etc., to avoid energy waste and possible damage, efficient start, and enhance resistance. sexual effect
Active Publication Date: 2018-04-06
JIANGHAN UNIVERSITY
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Abstract
The invention relates to a stress inducible promoter sequence which comprises a DNA nucleotide sequence of 1388bp of SEQ ID NO: 1 and belongs to the technical field of plant genetic engineering. The invention also provides a stress inducible promoter plant expression vector and a method of expressing a target gene of a plant at low temperature, drought and salt induction. The target gene replacedby a GUS gene is inserted into the plant expression vector comprising the promoter. The recombinant vector is imported into the target plant; and SEQ ID NO:2 and SEQ ID NO:3 are PCR primers which separately comprise ECoRI and HindIII digestion sites. The primers are suitable for amplifying the DNA sequence of SEQ ID NO: 1. The stress inducible promoter sequence provided by the invention can be used for efficient expression of a foreign gene under cold start, drought and salt stress, and is suitable for researches on plant genetic breeding and improvement.
Application Domain
Plant peptidesVector-based foreign material introduction +1
Technology Topic
Stress inducibleDigestion +9
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