Rhizobium anhuiense V14-2 and application thereof

A rhizobia, CCTCCM2017611 technology, applied in the field of microorganisms, can solve problems such as the effect may not be good, and achieve the effect of strong nitrogen fixation and strong resistance to stress

Inactive Publication Date: 2018-04-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen that the actual effect of rhizobia is closely related to the type of legumes and the environment in which the legumes grow. The rhizobia that works well on one legume may not be as effective on another legume. Very good, rhizobia that work well in one place may not work well in another place

Method used

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  • Rhizobium anhuiense V14-2 and application thereof
  • Rhizobium anhuiense V14-2 and application thereof
  • Rhizobium anhuiense V14-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation, purification and preservation of embodiment 1 rhizobia V14-2

[0022] From the wild purple sweet potato collected in Gongping Town, Wenjiang District, Chengdu City, Sichuan Province, select the large and plump red root nodules on the main roots of robust plants, wash the root nodules, and take part of the root bark, and carry out surface disinfection: soak in ethanol (95%) After 5 minutes, pour it out, then soak it in mercuric chloride (0.1%) for 5 minutes, and finally rinse it with sterile water for 6-8 times. Put the root nodules on the sterilized white porcelain plate in the ultra-clean workbench, clip the root nodules with a bamboo stick and smear them on the plate of YMA medium, perform streaking and purification, and culture in a 28°C incubator. YMA medium formula: KH 2 PO 4 0.25g, MgSO 4 `7H 2 O 0.2g, CaCl 2` 6H 2 O 0.1g, NaCl 0.1g, ammonium molybdate (1%) 2mL, boric acid (1%) 2mL, Congo red (1%) 2.5mL, mannitol 10g, yeast powder 0.8g, agar 18-20...

Embodiment 2

[0025] The back graft test of embodiment 2 rhizobia

[0026] The sand culture method was used for the backgrafting test of rhizobium. The variety of purple sweet potato used in the test was purple sweet potato, which was purchased from Chengdu Green Grass Garden Seed Industry Co., Ltd. Carry out in the light room (temperature control 22~24 ℃, light intensity about 2800 lux, sunshine time 14h), plant and harvest 46 days. Replenish sterilized nitrogen-free nutrient solution regularly, inoculate rhizobia V14-2 into a sand culture device, use a 300mL plastic cup for sand culture device, use vermiculite as the substrate, and use the same variety of plants that were not inoculated with rhizobia V14-2 as a control CK, each treatment was repeated three times. After harvesting, the number of nodules and the dry weight of plants were used to evaluate the inoculation effect of Rhizobium V14-2.

[0027] (1) Bacteria culture: inoculate rhizobia V14-2 in YMA liquid medium, place on a shak...

Embodiment 3

[0037] The stress resistance of embodiment 3 rhizobia V14-2

[0038] The stress resistance of Rhizobium V14-2 was mainly tested for salt tolerance, acid and alkali resistance. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control (CK). The above-mentioned YMA slant culture of rhizobia V14-2 was scraped and washed with sterile water to make a bacterial suspension for use. The spot inoculation method was used, and each treatment was repeated 3 times. For the tests of acid and alkali resistance and salt resistance, the plates for the determination of the suitable growth temperature range were observed and recorded after being cultured at 28°C for 4 days.

[0039] (1) Medium formula for acid and alkali resistance determination: take YMA medium as the basic medium, adjust the pH value with HCl and NaOH, so that the pH value of the medium is 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, 11.0 .

[0040] (2) Medium fo...

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Abstract

The invention discloses a Rhizobium anhuiense V14-2 and application thereof. The Rhizobium anhuiense V14-2 is preserved in CCTCC (China Center For Type Culture Collection) with a preservation number of NO:M2017611. The Rhizobium anhuiense V14-2 is a good vicia villosa Rhizobium anhuiense strain which has high symbiotic nitrogen fixing ability, obvious yield increasing effect on Sichuan vicia villosa, IAA (Indole Acetic Acid) secreting ability, organophosphorus and potassium dissolving abilities and high stress resistance. The yield of fresh grass of the vicia villosa can be increased by 29.5 percent and the yield of fresh root can be increased by 79.3 percent by inoculation of the Rhizobium anhuiense V14-2 without application of nitrogenous fertilizer.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to rhizobium V14-2 and application thereof. Background technique [0002] Viciavillosa L. is the main leguminous green manure crop in the rice area of ​​China. It is the main variety of vetch used as green manure and forage cultivation, and it is a high-quality organic fertilizer (Fu Yunzhang, 2017; Cao Weidong et al., 2010). It has the characteristics of tender stem juice, large leaves, high yield, good palatability, and rich nutrition. It is a high-quality green feed, a good substitute for concentrated feed and a compound feed raw material. It is popular in Sichuan, Yunnan, Guizhou and other breeding industries. Farmers' favorite (Guo Tailei, 2013; Shi Wanqing, 2016). In the planting process of purple sweet potato, chemical fertilizers are generally used to increase the yield. The study found that farmers in Yuexi County used 300 kg of farm soil miscellaneous fertilizer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/00A01P21/00C12R1/41C09K101/00
CPCA01N63/00C09K17/14C09K2101/00C12N1/205C12R2001/41
Inventor 徐开未陈远学彭丹全紫曼吴永成巫锐张小平
Owner SICHUAN AGRI UNIV
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