Preparation method of microbial fermented prawn feed

A technology for microbial fermentation and shrimp feed is applied in the field of preparation of microbial fermentation shrimp feed, which can solve the problems of increasing the difficulty of disease control, shrimp disease and death, and destroying the ecological balance, so as to treat intestinal flora disturbance and increase the immune system. function, the effect of increasing disease resistance

Inactive Publication Date: 2018-04-20
李六秀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of high-density industrialized shrimp farming, the pollution of high-protein substances in the feed to the water body, and the pollution of the water body by ammonia, nitrogen, and hydrogen sulfide formed by shrimp excrement often cause a large number of prawns to morbidity and death
For the disease prevention and control in the breeding process, the commonly used technical treatment methods are mainly the large-scale use of antibiotics, quaternary ammonium salts, iodine preparations and other disinfectants, which not only seriously damages the ecological balance, but also remains in the shrimp body, destroying the normal bacteria of the animal body. Group balance will lead to a decline in the immune function of animals, and will lead to the increase of drug-resistant strains in animals, further increasing the difficulty of disease prevention and control
Due to the lack of clear guidelines for use, the abuse of antibiotics and chemical drugs has posed a great threat to the ecological environment and human health for a long time

Method used

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  • Preparation method of microbial fermented prawn feed

Examples

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Effect test

Embodiment 1

[0027] A preparation method for microbial fermentation prawn feed, comprising the following steps:

[0028] S1. Mix and pulverize 20 parts by weight of mussels, 10 parts of river snails, and 20 parts of silkworm chrysalis, then add 200 parts by weight of water to grind into a slurry, then add 0.1 parts by weight of pepsin at a temperature of 35°C Enzymolysis under low temperature for 3 hours, then adding 0.1 parts by weight of papain and enzymolysis at a temperature of 50°C for 5 hours, then adding 0.5 parts by weight of flavor protease and enzymolysis at a temperature of 58°C for 5 hours, centrifuged to obtain the - filter residue and supernatant, then concentrate and spray dry the supernatant to obtain powder;

[0029]S2, the weight portion is 3 parts of Sophora flavescens, 2 parts of Eupatorium adenophorum, 3 parts of columbine, 3 parts of southern candle leaves, 4 parts of Corydalis dwarf, 3 parts of Sagebrush, 2 parts Achyranthes bidentata, 3 parts of leaking reeds, 4 pa...

Embodiment 2

[0041] The preparation method of microbial fermentation prawn feed comprises the following steps:

[0042] S1. Mix and pulverize 25 parts by weight of mussels, 15 parts of river snails, and 23 parts of silkworm chrysalis, then add 230 parts by weight of water to grind into a slurry, then add 0.2 parts by weight of pepsin at a temperature of 38°C Enzymolysis under low temperature for 4 hours, then adding 0.1 parts by weight of papain and enzymolysis at a temperature of 53°C for 5 hours, then adding 0.8 parts by weight of flavor protease and enzymolysis at a temperature of 60°C for 7 hours, centrifuged to obtain the - filter residue and supernatant, then concentrate and spray dry the supernatant to obtain powder;

[0043] S2, the parts by weight are 4 parts of Sophora flavescens, 4 parts of Eupatorium adenophorum, 4 parts of columbine, 4 parts of southern candle leaves, 6 parts of Corydalis dwarf, 5 parts of sagebrush, 3 parts of Achyranthes bidentata, 4 parts of leaking reeds,...

Embodiment 3

[0055] A preparation method for microbial fermentation prawn feed, comprising the following steps:

[0056] S1. Mix and pulverize 30 parts by weight of river mussels, 20 parts of river snails, and 25 parts of silkworm chrysalis, then add 250 parts by weight of water to grind into a slurry, then add 0.2 parts by weight of pepsin at a temperature of 40°C Enzymolysis under low temperature for 4 hours, then adding 0.2 parts by weight of papain for enzymolysis at a temperature of 53°C for 6 hours, then adding 1 part by weight of flavor protease for enzymolysis at a temperature of 62°C for 8 hours, and centrifuging to obtain the - filter residue and supernatant, then concentrate and spray dry the supernatant to obtain powder;

[0057] S2, the parts by weight are 5 parts of Sophora flavescens, 6 parts of Eupatorium adenophorum, 5 parts of columbine, 5 parts of southern candle leaves, 7 parts of Corydalis dwarf, 6 parts of sagebrush, 4 parts of Achyranthes bidentata, 6 parts of leaki...

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Abstract

The invention provides a preparation method of a microbial fermented prawn feed. The method comprises the following steps: step 1, mixing freshwater mussels, river snails and silkworm chrysalises, adding water, grinding the obtained mixture into slurry, adding pepsin and papain and carrying out enzymolysis so as to obtain first filter residues and supernatant; step 2, mixing lightyellow sophora roots, crofton weeds, garden columbine, leaves of oriental blueberry and herba corydalis, and conducting heating, refluxing and separation to obtain second filter residues and a filtrate; step 3, inoculating a primary culture medium with bifidobacterium bifidum, lactobacillus and bacillus natto and carrying out culturing; step 4, mixing corn meal, the first filter residues, the second filter residues, the filtrate, bacillus subtilis and clostridium butyricum, and conducting fermentation to obtain a first fermented product; mixing the first fermented product with clam shell powder, and conductingfermentation, filtration and drying to obtain a second fermented product; and step 5, mixing the second fermented product, compound vitamins and a powder, and conducting granulation. The prawn feed of the invention contains probiotics which can increase the immune system functions of the prawns, and can also treat intestinal flora disorder, improve intestinal environment, and promote intestinal patency.

Description

technical field [0001] The invention relates to the technical field of prawn feed, in particular to a preparation method of microbial fermented prawn feed. Background technique [0002] Shrimp farming has the advantages of fast growth, strong adaptability, and high yield, and has become one of the most important shrimp species in my country. However, in the process of high-density industrialized shrimp farming, the pollution of high-protein substances in the feed to the water body, and the pollution of the water body by ammonia, nitrogen, and hydrogen sulfide formed by shrimp excrement often cause a large number of prawns to morbidity and death. For the disease prevention and control in the breeding process, the commonly used technical treatment methods are mainly the large-scale use of antibiotics, quaternary ammonium salts, iodine preparations and other disinfectants, which not only seriously damages the ecological balance, but also remains in the shrimp body, destroying t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/12A23K10/22A23K10/20A23K10/14A23K10/30A23K10/37A23K10/18A23K10/26A23K20/147A23K20/111A23K20/174
CPCA23K10/12A23K10/14A23K10/18A23K10/20A23K10/22A23K10/26A23K10/30A23K10/37A23K20/111A23K20/147A23K20/174A23K50/80Y02A40/818Y02P60/87
Inventor 李六秀
Owner 李六秀
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