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Pharmaceutical applications of isocoumarins compounds separated from periplaneta americana extract

A technology of Periplaneta americana and isocoumarin, applied in medical preparations containing active ingredients, drug combinations, food science, etc., can solve the problems of elucidating the basis of active substances, complex components, and unclear active substances at the molecular level. Achieve the effects of promoting collagen secretion from fibroblasts, softening skin scars, and promoting granulation tissue proliferation

Inactive Publication Date: 2018-04-24
INNER MONGOLIA JINGXIN PHARMACEUTICAL CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex ingredients contained in the above products, the real active substances are still unclear
It is currently reported that the chemical components in Periplaneta americana are mainly pheromones, amino acids and various insect neuropeptides, in addition to cockroach acid, cockroach ketone, cockroach oil, allergen components, enzymes, cytochrome A, B, C, chitin, nicotinic acid and trace elements, etc. However, there are few reports on active chemical components with clear pharmacological effects
Most of the existing literature reports are the extracts and compositions of Periplaneta americana, but the basis of its active substances has not been clarified at the molecular level.

Method used

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  • Pharmaceutical applications of isocoumarins compounds separated from periplaneta americana extract
  • Pharmaceutical applications of isocoumarins compounds separated from periplaneta americana extract
  • Pharmaceutical applications of isocoumarins compounds separated from periplaneta americana extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Compounds 1-6

[0021] According to the method reported in Document 1, the isocoumarin compounds (compounds 1-6) shown in formula I were isolated from the extract of Periplaneta americana, and then the following pharmacological tests were carried out.

Embodiment 2

[0022] Example 2 Effect of p-xylene on mouse ear swelling

[0023] 90 male ICR mice weighing 21-23 g were randomly divided into 9 groups according to body weight: normal control group, model control group (25 mg·kg -1 ), compounds 1-6 (25mg·kg -1 ), positive control group (hydrocortisone 25mg·kg -1 ), 10 in each group. 30 minutes after each group was intraperitoneally injected with the corresponding drugs and solvents, except for the normal control group, 30 μl of xylene was evenly applied to the left ear of the mouse (15 μl on both sides of the front side). Ears were accurately weighed with an analytical balance, and the mouse ear swelling degree was calculated: (left ear-right ear) / right ear, and the results are shown in Table 1 below.

[0024] Table 1 The compound of the present invention is on the effect of xylene-induced mouse ear swelling ( n=10)

[0025]

[0026] Note: Compared with the normal control group, △ P△Δ P<0.01; compared with model control group, *P<...

Embodiment 3

[0028] Example 3 Effect on Promoting Fibroblast Collagen Secretion

[0029] Reagents and materials: Skin fibroblasts (ATCC-2522), PBS, Hank’s balanced salt solution, penicillin-streptomycin (double antibody) and fetal bovine serum (FBS) were purchased from Hyclone; DMEM (high glucose) medium , 0.25% trypsin (including EDTA) were purchased from Gibico Company; positive control transforming growth factor beta (transforming growth factor beta, TGF-β) was purchased from Peprotech Company; collagen ELISA kit was purchased from TaKaRa Company; MTS reagent was purchased from Promega Company.

[0030] Experimental method: on a 12-well cell culture plate, skin fibroblasts were mixed with 20 μM drug solution to be tested (compounds 1-6), and a blank control without drug and a TGF-β positive control were set at the same time. 37°C, 5% CO 2 After culturing for 3 days, collect the cell culture supernatant and store it at -80°C; add MTS, and use the MTS colorimetric method to detect the OD...

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PUM

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Abstract

The invention discloses pharmaceutical applications of isocoumarins compounds separated from periplaneta americana extract, especially the application in preparing medicines for treating or preventinginflammation, promoting granulation tissue hyperplasia or wound healing, and treating diseases relevant to wounded tissue repairing.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry and relates to a pharmaceutical application of isocoumarin compounds isolated from the extract of Periplaneta americana. Background technique [0002] Periplaneta americana (Periplaneta americana) is an insect of the genus Periplaneta in the class of Insecta Pterosae, Blattata, Blattedae, commonly known as "cockroach". Taste: salty, cold; Cure: blood stasis with hard cold and heat, broken accumulation, closed throat, internal cold without seeds". "Cockroach" is its common name first seen in "Compendium of Materia Medica Supplements". It is also known as Shi Jiang and Hua Chong. Different places have different names, such as Stealing Oil Woman and Zao Anzi. As a huge insect family, Blattidae has existed on the earth for 320 million years, and it is a creature that appeared in the same era as dinosaurs. It is one of the most vigorous, oldest, and most successful insect groups in the world. As a...

Claims

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Application Information

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IPC IPC(8): A61K31/366A61P29/00A61P17/02A23L33/10
Inventor 成亮刘力张琴其他发明人请求不公开姓名
Owner INNER MONGOLIA JINGXIN PHARMACEUTICAL CO LTD
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