Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection method of content of estradiol in anaerobic granular sludge

An anaerobic granular sludge and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult pretreatment and the existence of traces of estradiol, and achieve low detection limits, simple operation, and easy extraction The effect of low temperature

Pending Publication Date: 2018-05-01
BEIJING UNIV OF TECH
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to develop an analytical technique with high recovery rate, high sensitivity, simple operation and good reproducibility for the problems of complex anaerobic granular sludge matrix, difficult pretreatment, and traces of estradiol. , to achieve quantitative analysis and detection of trace estradiol in anaerobic granular sludge samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of content of estradiol in anaerobic granular sludge
  • Detection method of content of estradiol in anaerobic granular sludge
  • Detection method of content of estradiol in anaerobic granular sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Sludge sample extraction

[0038] The collected sludge sample is subjected to freeze-drying treatment, using methanol as an extractant, using an ultrasonic instrument for ultrasonic extraction, diluting the extract with methanol / distilled water=1:20 (v / v), centrifuging (preferably centrifuging at 10000rpm for 10min ), collect the supernatant, repeat twice, and the gained supernatant is combined;

[0039] (2) Purification of supernatant

[0040] After the above supernatant was vacuum filtered, the Oasis HLB 3cc (60mg) solid phase extraction column (SPE column) of Waters Company was used to purify and enrich the estradiol therein, and ethyl acetate was used to remove the estradiol from the solid phase extraction column. After elution, add an internal standard, blow dry with nitrogen, and derivatize to obtain the test solution;

[0041] (3) Analysis of estradiol content by gas chromatography-tandem mass spectrometry

[0042] Utilize gas chromatography-tandem mass sp...

Embodiment 2

[0062] Anaerobic granular sludge in an upflow anaerobic sludge bed reactor for treating estrogen-containing wastewater was collected, and the collected sludge samples were freeze-dried for 48 hours with a freeze dryer. Put the mud sample in a glass sample bottle, use methanol as the extractant, use an ultrasonic instrument to ultrasonically extract at a frequency of 20kHz for 15min, dilute the extract with methanol / distilled water = 1:20 (v / v), and centrifuge at 10000rpm for 10min , collect the supernatant, repeat twice, and combine the supernatant. The supernatant after above-mentioned merging adopts Waters company Oasis HLB 3cc (60mg) solid-phase extraction column (SPE column) enrichment wherein estradiol after vacuum suction filtration, and solid-phase extraction column is washed with 5ml acetic acid successively before use Ethyl ester, 5ml methanol, and 10ml ultrapure water were passed through the column for activation. After enrichment, use 5ml 50% methanol to rinse the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection method of content of estradiol in anaerobic granular sludge, and belongs to the technical field of sludge analysis. The method comprises the following steps: freeze-drying a collected anaerobic granular sludge sample; performing ultrasonic extraction on estradiol in the sludge sample by adopting methanol as an extraction agent; diluting extraction liquid, and then, performing high-speed centrifugation; performing vacuum suction filtration on supernatant liquid, and then, enriching estradiol in the supernatant liquid by adopting a solid-phase extraction column; eluting estradiol from the solid-phase extraction column by adopting ethyl acetate; performing nitrogen gas blow-drying concentration and derivatization to obtain liquid to be detected; and analyzing the concentration of estradiol by utilizing gas chromatography and tandem mass spectrometry in combination with an internal standard method. The method can serve as an alternative method of a rapidsolvent extraction method and can be used for measuring the content of estradiol in the sludge sample, and the recovery rate is 92.1+ / -2.4%.

Description

technical field [0001] The invention relates to a detection technology of estradiol content in anaerobic granular sludge, in particular to the detection technology of estradiol content in anaerobic granular sludge by ultrasonic extraction-solid phase extraction-gas chromatography-tandem mass spectrometry The detection method belongs to the technical field of sludge analysis. Background technique [0002] As a typical endocrine disruptor, estradiol (17β-Estradiol, E2) can interfere with the endocrine system of humans and wild animals at extremely low concentration levels (μg / l to ng / l), causing aquatic fish Hermaphrodites, feminization of male fish and so on. Relevant investigations and studies have shown that estradiol is distributed in sewage plant effluent, rainwater, well water, lakes, oceans, drinking water and other water bodies, posing a potential threat to ecological security. Research on the removal of estradiol in various water bodies, especially sewage, has attra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/06G01N30/08G01N30/88
CPCG01N30/06G01N30/08G01N30/88G01N2030/062G01N2030/8809
Inventor 赵白航陈洁王占钊孙琪
Owner BEIJING UNIV OF TECH