Preparation method of novel sepsis animal model

An animal model and sepsis technology, applied in the field of preparation of animal models of sepsis, can solve problems such as deviation from clinical practice, and achieve the effects of small human influence, simple preparation method and clear genetic information

Inactive Publication Date: 2018-05-04
CANGZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, animal models of sepsis were established, and the animals were all young and healthy, which deviated from clinical practice, which made it difficult for the results of animal model research to be applied in clinical sepsis treatment

Method used

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  • Preparation method of novel sepsis animal model
  • Preparation method of novel sepsis animal model
  • Preparation method of novel sepsis animal model

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Experimental program
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Effect test

preparation example Construction

[0029] see Figure 1-3 , the present invention provides a kind of technical scheme: a kind of preparation method of new sepsis animal model, adopts SPF level 8 weeks old Balb / c mouse, body weight 25-30g, after adaptive feeding 1 week, carry out experiment, its It is characterized in that it comprises the following steps;

[0030] Step 1: In the stage of adaptive feeding and modeling, the mice were free to eat and drink, and the light / dark alternate lighting was adopted at intervals of 12 hours. The serial number corresponding to each mouse is fixed and unique. Using the random number table method, they were equally divided into two large groups, female and male, and each large group was further divided into A (n=6), B (n=8), C (n=8), D (n= 8) 4 teams;

[0031] Step 2: Group A: control group, at each injection time point, give the same volume of normal saline as the drug and (or) bacteria;

[0032] Step 3: Group B: Cyclosporin A injection group: on day -5, -3, and -1, weigh...

Embodiment 1

[0039] 1. Experimental animals and feeding

[0040] SPF grade 8-week-old Balb / c mice weighing 25-30 g were used. Experimental animals were provided by the Experimental Animal Center of the Academy of Military Medical Sciences, license number: SCXK (Beijing) 2014-0013. Animals were fed adaptively for 1 week before the experiment. In the stage of adaptive feeding and model establishment, the mice had free access to food and water, and were illuminated alternately with light / dark at intervals of 12 hours, and the temperature and humidity were suitable.

[0041] 2. Experiment with other main materials

[0042] Cyclosporine A (MCE Company), Escherichia coli DH5α (preserved in our laboratory), normal saline for injection (Chenxin Pharmaceutical Co., Ltd.), disposable syringe (Shengguang Medical Products Co., Ltd.), tryptone (Oxoid Company ), yeast powder (Oxoid), sodium chloride (Sinopharm Chemical Reagent Beijing Co., Ltd.).

[0043] 3. Preparation method:

[0044] 3.1 On the ...

Embodiment 2

[0053] 1. Specimen collection

[0054] Both male and female groups were divided into four subgroups. Each subgroup was injected with bacteria or normal saline on the 0th day, starting 12 hours after the injection, and observed the living conditions of the animals. The abdominal body temperature of the mice became lower and the hair stood upright. 1. When there is no response to touch, give 5mg / kg chloral hydrate deep anesthesia, draw blood from the heart, and add part of the whole blood to a sterile collection tube with 50 μL of sterile heparin sodium injection in advance for blood bacterial culture , the other part of whole blood was added to a 1.5mL collection tube, placed at room temperature for 2 hours, centrifuged at 8000rpm for 5 minutes, and the serum was separated for the detection of serological indicators of tissue damage. Kidney, lung, spleen and other tissues, take the left lobe of liver, left kidney, and left lung, and try to ensure that the parts of the samples a...

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Abstract

The invention discloses a preparation method of a novel sepsis animal model. On the basis of implementing experiments on SPF-level Balb / c mice which are 8 weeks and 25-30g in weight and which undergoadaptive breeding for 1 week, the method is characterized by comprising the following steps: 1, in an adaptive breeding and molding stage, enabling the mice to freely take food and water; in an environment that light / dark alternate illuminating is implemented by an interval of 12h and temperature and humidity are proper, marking 60 Balb / c mice conforming to a standard, half male and half female, wherein each mouse has a corresponding fixed and unique serial number; with the adoption of a random number table method, equally dividing the mice into two major groups, namely a female group and a male group, and further dividing each major groups into four sub-groups, namely A (n=6), B (n=8), C (n=8) and D (n=8). The obtained novel model is intervened by such drugs as cyclosporin A and the like,resulting in a low immune function of the animals (the mice), which conforms to a circumstance that people, who are low in immune function due to such reasons as surgeries, age and the like, suffer from sepsis, so that a natural occurrence and development process of the sepsis can be simulated better.

Description

technical field [0001] The invention relates to the field of animal medical models, in particular to a preparation method of a novel sepsis animal model. Background technique [0002] Sepsis refers to the systemic inflammatory response syndrome caused by infection, the incidence rate remains high, and patients with this disease are very prone to complicated with multiple organ dysfunction. The pathogenesis of sepsis is complex, involving a series of problems such as inflammatory response, endothelial injury, and coagulation disorders. It is the number one killer of patients in intensive care units. Hundreds of thousands of people in Europe and the United States die of sepsis every year. Establishing a standardized animal model of sepsis is necessary to explore the pathophysiological mechanisms and preventive measures of severe systemic infection, septic shock and multiple organ dysfunction syndrome. [0003] Researchers have established a variety of sepsis animal models, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/13A61K35/74A61D1/00A61D7/00
CPCA61D1/00A61D7/00A61K35/74A61K38/13Y02A50/30
Inventor 霍景瑞孔宪斌刘英富张晶晶王蕾刘颖杨晓辉
Owner CANGZHOU MEDICAL COLLEGE
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