Manufacturing method and application of p-hydroxy benzoate molecular imprinting electrochemical sensor based on nano-silver modification

A technology of p-hydroxybenzoic acid and molecular imprinting, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of cumbersome sample pretreatment, unsuitable for wide application, complex equipment and high cost, and achieve the goal of broadening the detection linear range, The effect of low cost and convenient pre-treatment

Inactive Publication Date: 2018-05-04
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the equipment used in these methods is complex and expensive, the sample pretreatment is cumbersome, and the separation is slow, which is not suitable for wide application, especially poor selectivity, narrow linear range, and high minimum detection limit.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Immerse the pretreated glassy carbon electrode in the newly prepared nano-silver sol, take out the glassy carbon electrode after 20 minutes and dry it at room temperature to obtain a nano-silver modified glassy carbon electrode;

[0017] (2) Add o-phenylenediamine and p-hydroxybenzoic acid to the HAc-NaAc buffer solution with a molar concentration of 0.1mol / L and pH=5.2, and the molar concentration of o-phenylenediamine in the mixed solution is 0.02mol / L. The molar concentration ratio of hydroxybenzoic acid and o-phenylenediamine is 1:3;

[0018] (3) In the mixed solution obtained in step (2), continue to pass high-purity nitrogen gas for 20 minutes, and then use the nano-silver modified glassy carbon electrode obtained in step (1) as the working electrode, the saturated calomel electrode as the reference electrode and platinum The sheet electrode was immersed in the mixed solution as an auxiliary electrode to form a three-electrode body polymerization system, and e...

Embodiment 2

[0021] (1) Immerse the pretreated glassy carbon electrode in the newly prepared nano-silver sol, take out the glassy carbon electrode after 20 minutes and dry it at room temperature to obtain a nano-silver modified glassy carbon electrode;

[0022] (2) Add o-phenylenediamine and p-hydroxybenzoic acid to the HAc-NaAc buffer solution with a molar concentration of 0.1mol / L and pH=5.2, and the molar concentration of o-phenylenediamine in the mixed solution is 0.02mol / L. The molar concentration ratio of hydroxybenzoic acid and o-phenylenediamine is 1:2;

[0023] (3) In the mixed solution obtained in step (2), continue to pass high-purity nitrogen gas for 20 minutes, and then use the nano-silver modified glassy carbon electrode obtained in step (1) as the working electrode, the saturated calomel electrode as the reference electrode and platinum The sheet electrode is immersed in the mixed solution as an auxiliary electrode to form a three-electrode body polymerization system, and th...

Embodiment 3

[0026] (1) Immerse the pretreated glassy carbon electrode in the newly prepared nano-silver sol, take out the glassy carbon electrode after 20 minutes and dry it at room temperature to obtain a nano-silver modified glassy carbon electrode;

[0027] (2) Add o-phenylenediamine and p-hydroxybenzoic acid to the HAc-NaAc buffer solution with a molar concentration of 0.1mol / L and pH=5.2, and the molar concentration of o-phenylenediamine in the mixed solution is 0.02mol / L. The molar concentration ratio of hydroxybenzoic acid and o-phenylenediamine is 1:2;

[0028] (3) In the mixed solution obtained in step (2), continue to pass high-purity nitrogen gas for 20 minutes, and then use the nano-silver modified glassy carbon electrode obtained in step (1) as the working electrode, the saturated calomel electrode as the reference electrode and platinum The sheet electrode is immersed in the mixed solution as an auxiliary electrode to form a three-electrode body polymerization system, and th...

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Abstract

The invention discloses a manufacturing method and application of a p-hydroxy benzoate molecular imprinting electrochemical sensor based on nano-silver modification. The manufacturing method comprisesthe following steps: immersing a glassy carbon electrode into newly prepared nano-silver sol to modify; taking o-phenylenediamine as a functional monomer and taking hydroxybenzoic acid as a templatemolecule, obtaining an o-phenylenediamine polymer membrane imprinted by p-hydroxy benzoate molecules on the glass carbon electrode modified by nano-silver through electrochemical polymerization; and removing p-hydroxy benzoate template molecules from the o-phenylenediamine polymer membrane imprinted by p-hydroxy benzoate molecules to obtain the p-hydroxy benzoate molecular imprinting electrochemical sensor based on nano-silver modification. The manufacturing method expands detecting linear range of the sensor, remarkably improves sensitivity of the sensor, greatly reduces detection limit of the sensor, improves selectivity of the sensor and is strong in capacity of resisting disturbance. The method is simple, is convenient to operate, is low in cost, is convenient in detected sample pretreatment and is low in cost.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, and in particular relates to a preparation method and application of a p-hydroxybenzoic acid molecularly imprinted electrochemical sensor modified by nano-silver. Background technique [0002] P-hydroxybenzoic acid is an organic chemical raw material, which is widely used in food, medicine, cosmetics, pesticides and dyes, and is also widely used as a preservative and fungicide. P-Hydroxybenzoic acid belongs to phenolic acids, which is irritating, toxic and carcinogenic. If it is discharged into the environment improperly, it will cause serious environmental pollution. At present, the commonly used methods for detecting trace amounts of p-hydroxybenzoic acid are high performance liquid chromatography, spectrophotometry, and gas chromatography-mass spectrometry. However, these methods use complex equipment, high cost, complicated sample pretreatment, slow separation, and are not su...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor闫长领陈玉娟王新生刘玉民王公轲张瑞星
OwnerHENAN NORMAL UNIV