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Drug sensitivity kit, preparing method thereof and bacterium drug sensitivity detection method

A kit and drug susceptibility technology, applied in the fields of biochemical equipment and methods, microbial determination/inspection, biochemical instruments, etc., can solve the problems of inaccurate test results, high cost, long time consumption, etc., and achieve accurate test results and cost. Inexpensive, easy-to-use effects

Inactive Publication Date: 2018-05-08
WUHAN POLYTECHNIC UNIVERSITY
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AI Technical Summary

Problems solved by technology

In the prior art, drug susceptibility testing is often carried out through drug susceptibility kits, but the current drug sensitivity kits generally have outdated and single types of drugs, insufficient test operability, long time-consuming, inaccurate test results, or high cost. shortcoming

Method used

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  • Drug sensitivity kit, preparing method thereof and bacterium drug sensitivity detection method
  • Drug sensitivity kit, preparing method thereof and bacterium drug sensitivity detection method
  • Drug sensitivity kit, preparing method thereof and bacterium drug sensitivity detection method

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preparation example Construction

[0039] The present invention also proposes a method for preparing the above-mentioned drug-sensitivity kit, which includes a culture medium plate and a drug-sensitive paper sheet. In an embodiment of the method for preparing the drug-sensitivity kit provided by the present invention , the preparation method of the drug sensitivity kit comprises the following steps:

[0040] Step S10, preparing a culture medium to form a culture medium plate after the medium is solidified;

[0041] In step S10, the culture medium includes common nutrient agar medium, MacConkey agar medium, SS agar medium and TSA agar medium containing NAD, to form common nutrient agar plate, MacConkey agar plate, SS agar plates and NAD-containing TSA agar plates.

[0042] According to the pathological change characteristics of animal lesion tissue, the bacteria suspected to be infected are determined, and then the corresponding medium plate is selected for drug susceptibility test. For example, E. It is a str...

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PUM

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Abstract

The invention discloses a drug sensitivity kit, a preparing method thereof and a bacterium drug sensitivity detection method. The drug sensitivity kit comprises a box body, a culture medium slab, scraps of paper of drug sensitivity and a testing apparatus, the box body is internally provided with an accommodation cavity, the culture medium slab is arranged in the accommodation cavity and comprisesa common nutrient agar slab, a MacConkey agar slab, an SS agar slab and a tryptic soytone broth agar slab containing a coenzyme A, the scraps of paper of drug sensitivity are arranged in the accommodation cavity, and each scrap of paper of drug sensitivity correspondingly contains any one of tulathromycin, acetyl-isovaleryl tulathromycin, valnemulin, levofloxacin, lomefloxacin, ciprofloxacin, enrofloxacin, gentamicin, kanamycin, streptomycin, tylosin, tilmicosin, spectinomycin, florfenicol, ceftiofur, tiamulin, doxycycline, colistin, amoxicillin and lincomycin. The drug sensitivity kit has the advantages that the testing result is precise, the consumed time is short, the operation is simple, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a drug sensitivity kit, a preparation method thereof, and a bacterial drug sensitivity detection method. Background technique [0002] Some farms abuse antibiotics in animal production. For example, in order to improve the health effects of antibiotics, antibiotics are used for a long time and overdose, which leads to drug residues in animal food and endangers the health of consumers. With the improvement of people's food safety awareness and the government's increasing emphasis on food safety, the use of antibiotics in farms has become more and more standardized. Selecting sensitive drugs for pathogenic bacteria can achieve precise control of animal diseases, reduce drug treatment costs, and reduce animal health. Drug residues in food. [0003] Farm veterinarians can judge the type of animal diseases based on the clinical and pathological examination of animals. For bact...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/22C12Q1/18
CPCC12M23/20C12M41/46C12Q1/18
Inventor 熊江林吴灵英刘玉兰王锐邱银生丁斌鹰
Owner WUHAN POLYTECHNIC UNIVERSITY
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