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Hedyotis diffusa tissue culture technology

A technology of Hedyotis diffusa and tissue culture is applied in the field of tissue culture and seedling raising of Hedyotis diffusa

Inactive Publication Date: 2018-05-11
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The seeds are light in texture and unevenly spread, and the denser places will affect the growth;
[0006] (2) There are many weeds in the field, and there are counterfeit varieties that are easy to mix with herbs or seeds of Hedyotis diffusa;
[0007] (3) The field is affected by temperature and dry humidity, the germination rate and survival rate are low, and the growth rate is slow;
[0008] (4) Compared with tissue culture, there is no seedling proliferation stage

Method used

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  • Hedyotis diffusa tissue culture technology
  • Hedyotis diffusa tissue culture technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A tissue culture technique for Hedyotis diffusa, comprising:

[0048] The selection and disinfection steps of explants: select the Hedyotis diffusa plant that has grown for 4-5 months, and in late July, the whole plant is picked by the seeds of the roots of the plant (Hendyotis diffusa is sown in spring and harvested in autumn, and it blooms from the root to the top. Lace results, so when collecting seeds, try to pick the ones that are close to the root to ensure the maturity of the seeds), cut off the immature part of the top, dry the stems of the mature part of the seeds together, and then crush the seeds (use a gauze-wrapped mallet to crush the seeds) Press), remove the waxy seed shell, sieve out impurities and ash, then put it into a clean beaker and move it to the inoculation room, and sterilize it on a sterile operating table; first soak it with 75% medical alcohol for 30s (75% alcohol is relatively Other disinfection reagents are more thorough in disinfection and...

Embodiment 2

[0055] The difference between Example 2 and Example 1 is that in the selection and disinfection steps of the explants, the Hedyotis diffusa plant that has grown for 4-5 months is selected, and in the first ten days of August, the whole plant is picked from the seeds at the root of the plant. , cut off the immature part of the top, dry the stems of the mature part of the seed together, roll out the seeds, remove the waxy seed shell, sieve out impurities and ash, and then put them into a clean beaker and move them to the inoculation room. Disinfect on the sterile operating table; first soak in 75% medical alcohol for 60s, stir continuously with tweezers during soaking, then rinse with sterile water for 3 times; finally disinfect with 45% sodium hypochlorite for 8 minutes, rinse with sterile water for 5 times , to obtain sterile seeds, and put them in a sterile tray on the ultra-clean workbench for later use.

[0056] Others are identical with specific embodiment 1.

Embodiment 3

[0058] The difference between embodiment 3 and embodiment 1 is:

[0059] In the sowing step: sow the sterile seeds on the first 1 / 2MS medium with a high-temperature sterilized spoon, seal the lid after sterilizing the bottle cap, and then transfer it to the cultivation room for cultivation. The temperature of the cultivation chamber is 24- 26°C, the light intensity is 2000-2500lx, and the light time is 12 hours / day. After the seeds germinate, continue to cultivate until the seedlings are 2-3cm long, and obtain a first-class mother bottle; the composition of the first 1 / 2MS medium is as follows: nitric acid Potassium 1900mg / L, ammonium nitrate 1650mg / L, calcium chloride dihydrate 445mg / L, magnesium sulfate heptahydrate 375mg / L, potassium dihydrogen phosphate 170mg / L, potassium iodide 0.83mg / L, boric acid 6.2mg / L, tetrahydrate Manganese sulfate 23.0mg / L, zinc sulfate heptahydrate 9.0mg / L, sodium molybdate 0.25mg / L, copper sulfate pentahydrate 0.028mg / L, cobalt chloride 0.03mg / L,...

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Abstract

The invention discloses a hedyotis diffusa tissue culture technology. The hedyotis diffusa tissue culture technology comprises the following steps: selecting and disinfecting explants, sowing seeds, carrying out subculture, carrying out rooting culture, carrying out seedling exercising, domesticating and transplanting. According to the tissue culture technology disclosed by the invention, the seeds reserved for planting hedyotis diffusa which is identified to be a certified product are subjected to tissue culture; obtained pure germchits are transplanted on a sawdust medium without counterfeitseeds of the hedyotis diffusa, so that certified-product hedyotis diffusa and pure seeds can be obtained; the obtained pure seeds are artificially planted to obtain the certified-product hedyotis diffusa.

Description

technical field [0001] The invention relates to the technical field of tissue culture and seedling raising of Hedyotis diffusa, in particular to a tissue culture process of Hedyotis diffusa. Background technique [0002] Hedyotis diffusa Willd (Hedyotis diffusa Willd) is an annual herb of Rubiaceae, 15-50cm high, with slender roots, branches and white flowers. The stem is slightly square or flat cylindrical, smooth and glabrous, with many branches from the base. The flowering period is spring, and the seeds are small and yellowish brown. It can be harvested in two seasons a year, spring planting in August and autumn planting in November. It grows in areas with an altitude of 800 meters, and mostly grows in paddy fields, ridges and moist open land. It is mainly distributed in Fujian, Guangdong, Hong Kong, Guangxi, Hainan, Anhui, Yunnan and other areas in China; it tastes bitter, light, and cold in nature. In recent years, it has been found that Hedyotis diffusa has a good e...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/23A01G24/00
CPCA01H4/008A01G31/00A01H4/001
Inventor 许冬瑾严新顾晓波
Owner 康美药业(昆明)种质资源有限公司
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