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A method for producing feed additives by using cassava residue and its alcohol waste mash

A feed additive and alcohol waste mash technology, applied in the field of feed additive preparation, can solve the problems of low utilization rate of raw materials, low content of organic matter such as protein, etc., and achieve the effect of short fermentation cycle, more surface active functional groups, and shortened fermentation cycle

Active Publication Date: 2021-01-01
济南深蓝动物保健品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, the raw material utilization rate of feed additives prepared from cassava dregs and alcohol waste mash is too low, and the content of organic substances such as protein in the additives is low

Method used

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  • A method for producing feed additives by using cassava residue and its alcohol waste mash
  • A method for producing feed additives by using cassava residue and its alcohol waste mash

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A. According to the ratio of parts by weight, take 4 parts of cassava dregs, mix them with 0.4g / L yeast extract, 0.10g / L urea, 1.4g / L peptone, and 20~30mg / L calcium sulfate in warm water at 60°C to prepare 25% (W / V) cassava residue solution;

[0040] B. Add 5 times the amount of loaded ionic liquid biochar to the cassava residue solution to react for 2 hours, then add reverse micelles-enzyme and 3 parts of alcohol waste mash and mix evenly, carry out raw material hydrolysis at 45°C for 30 minutes, 2000r / min Centrifuge at the bottom, get the upper phase solution, and obtain the slurry;

[0041] Described reverse micelle-enzyme is composed of: the alpha-amylase of 214IU / g cassava flour and the saccharification enzyme of 398IU / g cassava flour;

[0042] C. Adjust the initial pH of the paste to be 5.5, then inoculate 8%% of the composite bacteria liquid, and ferment for 48h at 50°C to obtain a mixed material; the composite bacteria liquid is Bacillus subtilis, Bacillus lich...

Embodiment 2

[0053] A. According to the ratio of parts by weight, take 10 parts of cassava dregs, mix them with 0.6g / L yeast extract, 0.14g / L urea, 1.6g / L peptone, and 30mg / L calcium sulfate in warm water at 80°C, and prepare 35% (W / V) cassava residue solution;

[0054] B. Add 10 times the amount of loaded ionic liquid biochar to the cassava residue solution to react for 3 hours, then add reverse micelles-enzyme and 5 parts of alcohol waste mash and mix evenly, and carry out raw meal hydrolysis at 55°C for 60 minutes, 2500r / min Centrifuge at the bottom, get the upper phase solution, and obtain the slurry;

[0055] Described reverse micelle-enzyme is composed of: α-amylase of 224IU / g cassava flour and saccharification enzyme of 694IU / g cassava flour;

[0056] C. Adjust the initial pH of the paste to 6.0, then inoculate 10% of the composite bacterial solution, and ferment for 72 hours at 60°C to obtain a mixed material;

[0057] D. Dry the mixed material to a solid material with a water co...

Embodiment 3

[0067] A. According to the ratio of parts by weight, take 8 parts of cassava dregs, mix them with 0.5g / L yeast extract, 0.12g / L urea, 1.5g / L peptone, and 28mg / L calcium sulfate in warm water at 68°C to make 30% (W / V) cassava residue solution;

[0068] B. Add 8 times the amount of loaded ionic liquid biochar to the cassava residue solution to react for 3 hours, then add reverse micelles-enzyme and 4 parts of alcohol waste mash and mix evenly, and hydrolyze the raw meal at 50°C for 50 minutes, 2000r / min Centrifuge at the bottom, get the upper phase solution, and obtain the slurry;

[0069] Described reverse micelle-enzyme is composed of: the alpha-amylase of 264IU / g cassava flour and the glucoamylase of 392IU / g cassava flour;

[0070] C. Adjust the initial pH of the paste to 6.0, then inoculate 9% of the composite bacterial solution, and ferment at 58°C for 65 hours to obtain a mixed material;

[0071] D. Dry the mixed material to a solid material with a water content of 8%, w...

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Abstract

The invention provides a method for producing a feed additive from cassava slag and its alcohol waste fermenting mash liquor. The method comprises treating cassava slag by biochar loading an ionic liquid so that the dissolution rate of the cassava starch is improved, carrying out starch hydrolysis through reverse micelle-enzyme, mixing the hydrolysis product and cassava alcohol waste fermenting mash, carrying out inoculation with compound bacteria, carrying out fermentation and drying the fermented mixture to obtain the feed additive. The biochar loading an ionic liquid improves degradation efficiency, effectively degrades lignin and cellulose to produce starch and reduces a cost and concentrated mash effects of inhibiting bacteria. The reverse micelle can improve the enzyme activity and enzyme stability and improve the recovery rate of the ionic liquid. The feed additive has high protein content, reduces energy consumption, improves the utilization rate of raw materials, shortens thefermentation cycle and has a good development prospect.

Description

technical field [0001] The invention belongs to the technical field of feed additive preparation, and in particular relates to a method for producing feed additive by utilizing cassava residue and its alcohol waste mash. Background technique [0002] At present, feed additives are developing in the direction of high efficiency, safety, environmental protection, and multi-function, especially with the introduction of biotechnology, the feed additive industry will enter a new historical stage - the stage of biological feed additives, using industrial waste to produce biological feed, reducing waste It is an important issue to reduce the production cost of pollution. [0003] The production of ethanol by cassava fermentation is an important industry in my country. The production of ethanol from cassava raw materials produces a large amount of cassava dregs and waste mash after fermentation. Both are difficult to deal with and cause great environmental pollution. Therefore, new ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/14A23K10/16A23K10/37A23K10/38A23K20/147A23K20/24
CPCA23K10/12A23K10/14A23K10/16A23K10/37A23K10/38A23K20/147A23K20/24Y02P60/87
Inventor 罗绍庆
Owner 济南深蓝动物保健品有限公司
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