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Preparation method for yeast culture

A technology of yeast culture and yeast, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the dissolution of microorganisms, can solve the problems of bacterial infection, product quality that is difficult to meet standards, and cannot be popularized, etc., to reduce Pollution, the effect of suppressing the generation of miscellaneous bacteria

Inactive Publication Date: 2018-05-15
北京中科润之农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the reasons of equipment and energy consumption, the liquid fermentation process cannot be widely promoted
[0006] Solid fermentation method is to use natural substrate as carbon source or use inert substrate as solid support. The system is anhydrous or nearly anhydrous fermentation process. The growth of microorganisms and the products formed are all on the surface of the substrate. However, The main disadvantage of solid fermentation is that the bacteria are easily infected by bacteria after multi-stage culture and open fermentation, and the product quality is difficult to meet the standard

Method used

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  • Preparation method for yeast culture
  • Preparation method for yeast culture

Examples

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Embodiment 1

[0028] Expansion culture of strains: Inoculate Candida tropicalis and Saccharomyces cerevisiae into 25kg of liquid medium, place in a constant temperature shaking incubator, keep the ambient temperature at 32°C, and cultivate at 195r / min for 27 hours to obtain the expansion culture medium; , the liquid medium includes 0.2 parts of magnesium sulfate, 2 parts of ammonium sulfate, 1 part of sodium citrate, 6 parts of potassium dihydrogen phosphate, 4 parts of dipotassium hydrogen phosphate, 10 parts of peptone, 1 part of sodium chloride, and 1 part of potassium dihydrogen phosphate part, adjust the pH of the liquid medium to 5;

[0029] Liquid fermentation: Inoculate the expanded culture medium into 55 kg of liquid medium with an inoculation amount of 22%, and keep the ambient temperature at 29°C for aerobic fermentation for 64 hours to obtain a liquid strain solution;

[0030] Preparation of solid medium: first take 11kg of cross-linked sephadex, 1-(2-(β-D-glucopyranoseoxy)-4,6-...

Embodiment 2

[0034] Expansion culture of strains: Inoculate Candida tropicalis and Saccharomyces cerevisiae into 20kg of liquid medium, place in a constant temperature shaking incubator, keep the ambient temperature at 30°C, and cultivate at 190r / min for 30h to obtain the expansion culture medium; , the liquid medium includes 0.2 parts of magnesium sulfate, 2 parts of ammonium sulfate, 1 part of sodium citrate, 6 parts of potassium dihydrogen phosphate, 4 parts of dipotassium hydrogen phosphate, 10 parts of peptone, 1 part of sodium chloride, and 1 part of potassium dihydrogen phosphate part, adjust the pH of the liquid medium to 6;

[0035] Liquid fermentation: Inoculate the expanded culture medium into 50 kg of liquid medium with an inoculation amount of 24%, and keep the ambient temperature at 29°C for 72 hours of aerobic fermentation to obtain a liquid strain solution;

[0036] Preparation of solid medium: first take 10kg of cross-linked sephadex, 1-(2-(β-D-glucopyranoseoxy)-4,6-dihydr...

Embodiment 3

[0040]Strain expansion culture: inoculate Candida tropicalis and Saccharomyces cerevisiae into 30kg of liquid medium, place in a constant temperature shaking incubator, keep the ambient temperature at 30°C, and cultivate at 190r / min for 30h to obtain the expanded culture medium; , the liquid medium includes 0.2 parts of magnesium sulfate, 2 parts of ammonium sulfate, 1 part of sodium citrate, 6 parts of potassium dihydrogen phosphate, 4 parts of dipotassium hydrogen phosphate, 10 parts of peptone, 1 part of sodium chloride, and 1 part of potassium dihydrogen phosphate part, adjust the pH of the liquid medium to 5;

[0041] Liquid fermentation: Inoculate the expanded culture solution into 60kg of liquid medium with an inoculation amount of 20%, and keep the ambient temperature at 30°C for aerobic fermentation for 72 hours to obtain a liquid strain solution;

[0042] Preparation of solid medium: first take 10kg of cross-linked sephadex, 1-(2-(β-D-glucopyranoseoxy)-4,6-dihydroxyp...

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Abstract

The invention relates to a preparation method for yeast culture, belongs to the field of yeast culture, and aims to solve the problem that infectious microbes in a yeast culture preparation process are more. The preparation method for yeast culture comprises the steps of culture enlarged cultivation, liquid fermentation, solid fermentation, finished product obtaining and the like, wherein a solidculture medium is prepared from the following raw materials: stalks, corn powder, bran, cross-linked sephadex, 1-(2-(beta-d-pyranglucoxy)-4,6-dihydroxy phenyl)-3-(4-hydroxyl phenyl)-acetone, D-2,3,5,6-tetrahydroxy-2-hexenic acid-gamma-lactone. The preparation method greatly reduces pollution of infectious microbes, and cell content obtained by wall breaking of yeast cells, cell walls, a yeast metabolic end product and a culture medium in the prepared yeast culture define a bioactive feed additive.

Description

technical field [0001] The present invention relates to the field of yeast culture, more specifically, it relates to a preparation method of yeast culture. Background technique [0002] Antibiotics are widely used as growth-promoting agents in animal feed, which has a positive effect on animal husbandry production. However, long-term use of antibiotics will cause problems such as drug resistance and endogenous infection in animals, which will gradually limit the application of antibiotics. Yeast has been used in animal production for a hundred years. Traditional feed yeast is used as a growth factor for rumen microorganisms and is often used in ruminants. But yeast is a relatively fragile living organism, which is easily inactivated by temperature, humidity and rough handling, and can even be damaged or killed by rumen acid. Consequently, the role of yeast in animal nutrition has also changed. [0003] Yeast products derived from yeast are green, natural, and pollution-fre...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/06A23K10/16C12R1/865C12R1/74
CPCA23K10/16C12N1/063C12N1/16
Inventor 张伶燕李卫军于洪恩
Owner 北京中科润之农业科技发展有限公司
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