Pesticide synergist
A technology of synergist and synergist, which is applied in the field of pesticides, can solve the problems of low efficiency and poor stability, and achieve the effects of improving effectiveness, improving resistance, and promoting germination and growth
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Embodiment 1
[0033] Acquisition and disinfection of Prunus chinensis: take Prunus chinensis and wash, cut the rhizomes into 2cm pieces, dry at 20°C for 2 hours, soak in 0.1% Tween-20 for 20 seconds, and rinse with 5% sodium hypochlorite solution for 6 min, washed 3 times with sterile water, and the pretreated heavy building was obtained.
[0034] Separation medium of Pachyphylla: in parts by mass, take 2 parts of cellulose, 2 parts of sodium pyruvate, 0.8 part of proline, 0.1 part of potassium nitrate, 0.1 part of MgSO4 7H2O, 0.2 part of K2HPO4, 0.5 part of CaCl2, 0.01 parts of FeSO4·7H2O, 15 parts of agar, 1000 parts of water, pH7.2, sterilized at 121°C for 20min.
[0035] Purification medium: in parts by mass, take 4 parts of glucose, 3 parts of yeast extract, 5 parts of malt extract, 1 part of NaCl solution with a mass fraction of 0.1%, 15 parts of agar, 1000 parts of double distilled water, pH 7.2, 121°C Sterilize for 20 minutes.
[0036] Fermentation medium: in parts by mass, take 5...
Embodiment 2
[0044] Acquisition and disinfection of Prunus chinensis: take Prunus chinensis and wash, cut the rhizome into 2cm pieces, dry at 22°C for 2 hours, soak in 0.1% Tween-20 for 25 seconds, and then rinse with 5% sodium hypochlorite solution for 6 min, washed 3 times with sterile water, and the pretreated heavy building was obtained.
[0045] Separation medium of Pachyphylla: in parts by mass, take 2 parts of cellulose, 2 parts of sodium pyruvate, 1.0 part of proline, 0.2 part of potassium nitrate, MgSO 4 ·7H 2 O 0.2 parts, K 2 HPO 4 0.3 parts, CaCl 2 0.6 parts, FeSO 4 ·7H 2 0.012 parts of O, 17 parts of agar, 1000 parts of water, pH7.3, sterilized at 121°C for 20min.
[0046] Purification medium: in parts by mass, take 5 parts of glucose, 4 parts of yeast extract, 6 parts of malt extract, 1 part of NaCl solution with a mass fraction of 0.1%, 17 parts of agar, 1000 parts of double distilled water, pH 7.3, 121°C Sterilize for 20 minutes.
[0047] Fermentation medium: in par...
Embodiment 3
[0055] Collection and disinfection of Prunus chinensis: take Prunus chinensis and wash, cut the rhizomes into 3cm pieces, dry at 25°C for 3 hours, soak in 0.1% Tween-20 for 30s, and rinse with 5% sodium hypochlorite solution for 7 minutes , washed 4 times with sterile water to obtain the pretreated heavy floor.
[0056] Separation medium of Pachyphylla: in parts by mass, take 3 parts of cellulose, 3 parts of sodium pyruvate, 1.2 parts of proline, 0.25 parts of potassium nitrate, MgSO 4 ·7H 2 O 0.3 parts, K 2 HPO 4 0.4 parts, CaCl 20.8 parts, FeSO 4 ·7H 2 0.02 parts of O, 20 parts of agar, 1000 parts of water, pH7.4, sterilized at 121°C for 20min.
[0057] Purification medium: in parts by mass, take 6 parts of glucose, 5 parts of yeast extract, 8 parts of malt extract, 2 parts of NaCl solution with a mass fraction of 0.1%, 20 parts of agar, 1000 parts of double distilled water, pH7.5, 121 Sterilize at ℃ for 20min.
[0058] Fermentation medium: in parts by mass, 60 parts...
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