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A kind of phylloside and its extraction method and application

A technology of pueruloside and extraction method, applied in the field of medicine, can solve the problems of complex and diverse components of traditional Chinese medicine, and achieve the effect of good research and development prospects

Active Publication Date: 2020-07-10
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the composition of traditional Chinese medicine is complex and diverse, and the material components that exert medicinal effects in Physalis edulis need to be studied. It is necessary to explore and develop this plant with medicinal value (ie, Physalis edulis)

Method used

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  • A kind of phylloside and its extraction method and application
  • A kind of phylloside and its extraction method and application
  • A kind of phylloside and its extraction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 (extracting and isolating active compound from the stem and leaf of Physalis edulis)

[0041] The extracting method of lanatoside, comprises the steps:

[0042] (1) Take Physalis physalis (Physalis puscens L., an annual herb of Physalis family Solanaceae) dry stem and leaf (9.3 kg) as raw material, add 8 mass times of the raw material with a volume fraction of 75% ethanol aqueous solution, and reflux extraction 2 times, 2 hours each time, combined the extracts, recovered the solvent under reduced pressure, and obtained the total extract (1170.0g) after concentration;

[0043] (2) total extract is dispersed into the water of 4 mass times, extracts with sherwood oil of equal volume, ethyl acetate, n-butanol successively, n-butanol extract decompresses and reclaims solvent, obtains n-butanol layer extract ( 200.0g);

[0044] (3) Get the n-butanol layer extract (100g) and separate it through silica gel column chromatography. The volume ratios are: 100:1, 70:1...

Embodiment 2

[0056] Embodiment 2 (extract and isolate active compound from Physalis edulis fruit)

[0057] The extracting method of lanatoside, comprises the steps:

[0058] (1) Take Physalis physalis (Solanaceae Physalis genus Physalis puscens L.) fruit (17.5kg) as raw material, and the volume fraction of 8 mass times of raw materials added is 75% Aqueous ethanol solution, reflux extraction 2 times, each 2 hours, combined extraction solution, reclaim solvent under reduced pressure, obtain total extract (1420.0g) after concentrating;

[0059] (2) total extract is dispersed into the water of 4 mass times, extracts with sherwood oil of equal volume, ethyl acetate, n-butanol successively, n-butanol extract decompresses and reclaims solvent, obtains n-butanol layer extract ( 70.0g);

[0060] (3) The n-butanol layer extract is separated by silica gel column chromatography, and the volume ratios are: 100:0, 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40 :1, 30:1, 20:1, 10:1 dichloromethane-methanol ...

Embodiment 3

[0065] Example 3: Activity test of compounds inhibiting the release of nitric oxide (NO) from mouse macrophage RAW 264.7.

[0066] Take mouse macrophages (ATCC, TIB-71) that are in good growth state and in the logarithmic growth phase, and the concentration is 1 × 10 6 Cells / well of mouse macrophages were seeded in 96-well plate, 100 μL per well, at 37°C, and 5% CO 2 Routine cultivation in the incubator for 24 h. The culture medium was discarded from the cell plate after 24 h of cultivation, and LPS medium containing different drug concentrations and 2 μg / mL was added, and three parallel wells were set up in each group. Add 1 group containing only LPS medium), and replicate 3 wells in parallel. The positive drug control group is hydrocortisone, and cultured at 37°C for 24h. After 24 hours, take the medium supernatant, add Griess reagent [0.1% N-naphthylethylenediamine hydrochloride (50 μ L); 1% H of p-aminobenzenesulfonamide 3 PO 4 solution (5%, 50 μL), and measure the abs...

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Abstract

The invention discloses physapubescin, and an extraction method and application thereof. The physapubescin has a structure as shown in a formula (I) which is described in the specification. Experimental results show that the physapubescin as shown in the formula (I) and physapubescin aglycon as shown in a formula (II) can inhibit the activity of lipopolysaccharide in induction of mouse macrophagesto release nitric oxide, so it is proved that the physapubescin as shown in the formula (I) and the physapubescin aglycon as shown in the formula (II) can be used as anti-inflammatory drugs; and theresults of activity evaluation via an in-vitro antitumor activity screening system show that the physapubescin as shown in the formula (I) and the physapubescin aglycon as shown in the formula (II) can effectively inhibit the growth of hormone-independent prostate cancer cells, different types of renal cancer cells and human malignant melanoma cells, so it is proved tha the physapubescin as shownin the formula (I) and the physapubescin aglycon as shown in the formula (II) can be used as medicines for treating tumors and have good research and development prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a kind of lanatophyllin, its extraction method and its application in the preparation of anti-inflammatory and anti-tumor drugs. Background technique [0002] Physalispubescens L. is native to South America, and is mainly distributed in Northeast my country and Inner Mongolia in China (Chinese Academy of Sciences Chinese Flora Editorial Committee. Chinese Flora [M]. Beijing, Science Press Volume 1, 2004:525- 529), recorded in "Chinese Medicinal Materials Science" edited by Xu Guojun, the fruit of Physalis trichomes with persistent calyx is used as lantern herbal medicine. The nature and taste are sour and flat, and enter the lung meridian. It has the effects of clearing heat and detoxification, clearing throat and throat, promoting stasis and dispelling stagnation, diuresis and hemostasis. It is mainly used for sore throat, mumps, urinary tract inflammation, dysuria, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61K31/7048A61P29/00A61P35/00
CPCC07J17/00C07J17/005
Inventor 邱峰夏桂阳康宁陈丽霞
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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