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Preparation method for high-activity lactobacillus solid preparation

A lactic acid bacteria, highly active technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of increased cost, high price, complex growth and reproduction of lactic acid bacteria, etc.

Inactive Publication Date: 2018-05-18
SUZHOU KUNLAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The growth and reproduction of lactic acid bacteria requires complex nutrients. Traditional lactic acid bacteria fermentation mostly uses MRS medium, which is expensive
At the same time, due to the lack of nutrients and the accumulation of metabolites (mainly lactic acid) in the later stage of fermentation inhibit the growth of bacteria, resulting in low fermentation levels and increased costs in disguise
[0004] Lactic acid bacteria are easily inactivated during production, processing, storage and application, and have poor resistance to high temperature, gastric acid, bile salts, etc., especially during high-temperature processing and passing through the gastric environment, they will die in large numbers, thereby reducing biological activity. , leading to poor repeatability and stability of the use effect

Method used

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  • Preparation method for high-activity lactobacillus solid preparation

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preparation example Construction

[0022] The preparation method of the highly active lactic acid bacteria solid preparation of one embodiment, comprises the following steps:

[0023] Step S100, taking lactic acid bacteria strains and inoculating them in the sterilized seed culture medium according to the inoculum amount of 5-10%, standing and culturing at 30-40°C for 15-20 hours, and obtaining the seed liquid after culturing;

[0024] Among them, the ingredients of the seed medium are: molasses 60~80g / L, yeast extract 10~15g / L, corn steep liquor 10~15g / L, potassium dihydrogen phosphate 1~2g / L, magnesium sulfate 0.5~1g / L, Manganese sulfate 0.2~0.5g / L, Tween-80 0.5~1%, calcium carbonate 8~10g / L;

[0025] Preferably, the lactic acid bacteria strain is any one of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, and Bifidobacterium;

[0026] Preferably, the ingredients of the seed medium are: molasses 70g / L, yeast extract 12g / L, corn steep liquor 15g / L, potassium dihydrogen phosphate 2...

Embodiment 1

[0047] A preparation method for a solid preparation of highly active lactic acid bacteria, comprising the following steps:

[0048] (1) Take Lactobacillus acidophilus strains and inoculate them into the sterilized seed medium according to the inoculum amount of 5%, and culture them at 40°C for 15 hours, and obtain the seed liquid after cultivation;

[0049] Among them, the ingredients of the seed medium are: molasses 80g / L, yeast extract 15g / L, corn steep liquor 15g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 1g / L, manganese sulfate 0.5g / L, Tween- 80 1%, calcium carbonate 10g / L;

[0050] (2) Inoculate the seed liquid into the fermentation medium with an inoculation amount of 15%, and cultivate it at 40°C for 48 hours to obtain a mature fermentation liquid. During the fermentation process, the stirring speed is 50rpm. Add molasses at a ratio of 10g / hour to the base flow, and at the same time control the pH to 4.5 by adding calcium hydroxide to the flow, and contr...

Embodiment 2

[0057] A preparation method for a solid preparation of highly active lactic acid bacteria, comprising the following steps:

[0058] (1) Take the Lactobacillus plantarum strain and inoculate it into the sterilized seed medium at an inoculum amount of 10%, and culture it at 30°C for 20 hours, and obtain the seed liquid after cultivation;

[0059] Among them, the ingredients of the seed medium are: molasses 80g / L, yeast extract 15g / L, corn steep liquor 10g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 0.5g / L, manganese sulfate 0.2g / L, Tween -80 1%, calcium carbonate 8g / L;

[0060] (2) Inoculate the seed liquid into the fermentation medium with 10% inoculum amount, and cultivate it at 30°C for 40 hours to obtain a mature fermentation liquid. During the fermentation process, the stirring speed is 30 rpm, and after the pH of the fermentation liquid is reduced to 4.5, culture it at a rate of 1 liter. Add molasses at a ratio of 10 g / hour to the base flow, and at the same ...

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Abstract

The invention provides a preparation method for a high-activity lactobacillus solid preparation. With syrup and corn pulp as main carbon source and nitrogen source, the preparation method reduces medium cost, and by means of a three-stage pH regulation technique, the preparation method greatly increases the fermentation level. The liquid part which is obtained after fermentation broth is centrifuged is treated and then recycled, consequently, the effective metabolic product of lactobacillus is kept, and moreover, lactobacillu is protected well. The preparation method disclosed by the inventionhas the advantages of simple process, easiness in operation and high production efficiency, and furthermore, prepared lactobacillus is high in viable count, highly stable and easy to store.

Description

technical field [0001] The invention relates to the technical field of production of lactic acid bacteria, in particular to a preparation method of a solid preparation of highly active lactic acid bacteria. Background technique [0002] Lactic acid bacteria maintain the ecological balance of various probiotics in regulating the intestinal tract of the body, reduce the concentration of cholesterol in the serum, and metabolize the lactic acid produced to reduce the acidity of the surrounding environment, inhibit the growth and reproduction of bacteria in the intestinal tract, and promote Absorption and utilization of nutrients. Therefore, lactic acid bacteria are widely used in various industries such as light industry, food industry, medicine and feed industry. [0003] The growth and reproduction of lactic acid bacteria requires complex nutrients. Traditional lactic acid bacteria fermentation mostly uses MRS medium, which is expensive. At the same time, due to the lack of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/23C12R1/25C12R1/225C12R1/01
CPCC12N1/20
Inventor 赵路吴鹏刘晓峰赵岩宋宵因刘宝龙杨少飞
Owner SUZHOU KUNLAN BIOTECH
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