Sulforaphen extraction process

Abstract

The invention provides a sulforaphen extraction process, which comprises the following steps of taking dried broccoli seeds; performing peeling and powder grinding to obtain dry powder; then, mixing the dry powder and water; performing enzymolysis in a water bath; then, adding methanol for ultrasonic extraction to obtain extraction liquid; taking extraction liquid to perform centrifugal treatment;taking supernate; performing freeze drying to obtain the sulforaphen. The method provided by the invention has the advantages that only the water and the methanol need to be used; then, through the reasonable allocation steps, high-purity sulforaphen can be obtained through extraction; the addition of other organic reagents is not needed; the residue of other toxic and harmful substances can be avoided; the extraction cost is reduced; the benefits are improved; the extraction time is shortened; the degradation speed of the sulforaphen in the seeds in the extraction process is reduced; the operation is very simple and convenient.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a sulforaphane extraction process. Background technique [0002] Sulforaphane (1-isothiocyanate-4-methylsulfonylbutane) (Sulforaphane), also known as "sulforaphane", is relatively abundant in cruciferous plants such as broccoli, kale, and northern round carrots. Rich in antioxidants, it is also the plant active substance with the best anti-cancer effect found in vegetables. A large number of experiments have proved that sulforaphane has a good preventive effect on esophageal cancer, breast cancer, lung cancer, colon cancer, etc., especially it can kill the Helicobacter pylori that causes gastric ulcer and most gastric cancers. Yuesheng Zhang reported that by feeding sulforaphane to newborn 6-day-old female mice, the production of type II detoxification enzymes—glutathione transferase (GST) and quinone reductase (QR) can be induced in various tissue...

AI Technical Summary

Problems solved by technology

The chemical synthesis method has a complex synthesis process, high product yield and low cost, and the use of toxic or environmentally friendly reaction reagents in the synthesis process

In addition, synthetic products are less safe than naturally extracted products

However, the existing method for extracting sulforaphane uses more chemical reagents, and the obtained product will have more chemical residues, and the extraction efficiency is low, which is not conducive to large-scale production and use.

The previous extraction process also includes enzymatic hydrolysis, which requires the addition of expensive exogenous myrosinase preparations during the extraction process, resulting in high production costs of sulforaphane, so this method is limited in the actual production process

[0004] In addition, organic solvent extraction is often used in the art. The organic solvents used in the extraction include acetone, dichloromethane, sherwood oil, ethyl acetate and other toxic reagents. These toxic reagents will inevitably be present in the final product during the extraction process. Residues, causing harm to human health and restricting product use

In addition, some are extracted from broccoli buds and broccoli, which is not only cumbersome to operate, but also the extraction rate is not as high as that directly extracted from seeds

Images