Bacillus velezensis and application thereof in prevention and control of watermelon fusarium wilt

A watermelon wilt and bacillus technology, applied in the direction of application, bacteria, and fungicides, can solve problems such as adverse effects on the soil environment, and achieve strong stress resistance, fast growth, and high-efficiency control effects

Active Publication Date: 2018-06-01
山东戴盟得生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of highly toxic pesticides has led to serious drug resistance of the pathogen. General chemical control can no longer solve the problem, but has brought adverse effects on the soil environment.

Method used

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  • Bacillus velezensis and application thereof in prevention and control of watermelon fusarium wilt
  • Bacillus velezensis and application thereof in prevention and control of watermelon fusarium wilt
  • Bacillus velezensis and application thereof in prevention and control of watermelon fusarium wilt

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Isolation and purification of Bacillus velezensis XT1-3

[0030] Bacillus velezensis (B.velezensis) XT1-3 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from lake mud, and separation method is:

[0031] (1) Isolation of Bacillus

[0032] Lake mud samples were collected from Dongping Lake, Tai'an City, Shandong Province on April 21, 2013. Weigh 1g of soil sample (lake mud) into 100mL sterile water, place in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution was spread on the LB medium plate, and each gradient was spread in three parallels. After culturing at 28°C for 2 days, different forms of microbial colonies on the beef extract medium were picked and streaked on the beef extract medium plate. Regularly observe the colony growth. Then, the bacillus strains were purified by the streaking method on the plate, and the...

Embodiment 2

[0048] 1. Bacillus fermentation process

[0049] The formula of the beef extract liquid medium is: 3g of beef extract, 2.5g of glucose, 5g of sodium chloride, 1000mL of distilled water, and the pH is adjusted to 7.2-7.4.

[0050] Solid medium formula: solid material, inorganic salt and water, the mass ratio of described solid material and inorganic salt is 98.5: 0.5; Described solid material is counted as corn flour by mass ratio: soybean meal flour: bran=65: 25: 10. The composition and weight ratio of the inorganic salt are: potassium dihydrogen phosphate 15%, magnesium sulfate 5%, ammonium sulfate 15%, light calcium carbonate 65%. Preparation method: adding an appropriate amount of water (10-15 times the quality) to dissolve the inorganic salt to obtain an inorganic salt solution; mixing the inorganic salt solution with the solid material, and adding water to finally adjust the water content of the solid medium to 40-60%, so that the Hold the solid medium in your hand, and ...

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Abstract

The invention discloses bacillus velezensis and application thereof in prevention and control of watermelon fusarium wilt. The strain is a bacillus velezensis strain XT1-3, is obtained by being separated from the lake mud of the Dongping Lake in Tai'an City of Shandong province, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 26, 2017, and has a preservation number of CGMCC No. 14746. Test results prove that the bacillus velezensis strain XT1-3 biological control preparation is remarkable in prevention and control effect of the watermelon fusarium wilt, and the prevention and control effect reaches 77.03%; furthermore, after the bacillus velezensis is used, watermelons grow robustly and are protected from pesticide hazard.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. Specifically, the present invention relates to a strain of Bacillus Velez and its application in the prevention and treatment of watermelon wilt, and a biological control agent prepared by using the Bacillus Velez. Background technique [0002] Watermelons have always played an important role in the world's horticultural industry. China's watermelon planting area accounts for about 55% of the world's total area, and its total output accounts for more than 70%. In recent years, due to the continuous expansion of watermelon planting area, it is difficult to rotate crops, and the diseases represented by watermelon wilt are also increasing year by year. Watermelon wilt is a devastating soil-borne disease caused by Fusarium oxysporum f.sp.niveum, which is distributed in various watermelon producing areas in the world. Fusarium wilt of watermelon is a vascular disease that ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 王兆臣牛赡光张薇孙淑建王花
Owner 山东戴盟得生物科技有限公司
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