Composite microorganism bacterial agent for ginger, and preparation method and application thereof

A compound microbial inoculum and microorganism technology, applied in the field of agricultural microorganisms, can solve problems such as soil compaction, environmental pollution, and fertility decline, and achieve the effects of improving the ecological environment, improving biological yield and quality, and reducing continuous cropping obstacles.

Active Publication Date: 2018-06-08
北大荒农服华农(北京)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fertilizers used by ginger are mainly chemical fertilizers, which cause soil problems such as soil compaction and fertility decline; the prevention and control of plant diseases and insect pests mainly uses chemical pesticides, which not only easily produce drug resistance, but also cause serious environmental pollution. There is no compound microbial agent specially designed to reduce the obstacle of continuous cropping of ginger

Method used

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  • Composite microorganism bacterial agent for ginger, and preparation method and application thereof
  • Composite microorganism bacterial agent for ginger, and preparation method and application thereof
  • Composite microorganism bacterial agent for ginger, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Composition of special compound microbial agent for ginger:

[0035]

[0036]

[0037] The preparation method of above-mentioned special-purpose compound microbial inoculum for ginger comprises the following steps:

[0038] 1) Slant culture: Inoculate seven solid microorganisms with Bacillus jicheiformis SMrs15, Bacillus laterosporus LEs15, Bacillus megaterium FBs03, Bacillus subtilisFBrs14, Bacillus atrophaeus BHr4-8, Bacillus coagulans BHrs7-15, and Paenibacillus SM polymy2 respectively. Culture medium, activated strain;

[0039] 2) First-level seed cultivation: inoculate the activated strains in step 1) respectively in liquid culture medium under aseptic conditions, and cultivate them on a shaker at 180 rpm;

[0040] 3) Mixed fermentation culture: inoculate the primary seeds of step 2) into the liquid medium, and carry out the shaker culture of the compound microbial agent to obtain the special compound microbial bacterial agent for ginger.

[0041] In the ab...

Embodiment 2

[0046] Composition of special compound microbial agent for ginger:

[0047]

[0048] The difference between the preparation method of the bacterial agent and Example 1 is:

[0049] In step 1), the seven bacterial strains were inoculated on NA solid medium at 30°C and cultured for 3 days;

[0050] In step 2), seven kinds of bacterial strains were inoculated in NA liquid medium for 3 days on a shaking table at 30°C; counted with a hemocytometer, and the concentration of each bacterial strain reached 1.0×10 8 More than cfu / mL;

[0051] In step 3), the inoculum size is 0.5% of the volume of the liquid medium;

[0052] In step 3), the mixed fermentation culture conditions: temperature 30° C.; 180 rpm; culture for 4 days.

Embodiment 3

[0054] Composition of special compound microbial agent for ginger:

[0055]

[0056] The preparation method of the bacterial agent is the same as in Example 1. The difference is that:

[0057] In step 1), the seven bacterial strains were inoculated on NA solid medium at 37°C and cultured for 2 days;

[0058] In step 2), seven kinds of bacterial strains were inoculated in NA liquid medium for 3 days on a shaking table at 37°C; counted with a hemocytometer, and the concentration of each bacterial strain reached 1.0×10 8 More than cfu / mL;

[0059] In step 3), the inoculum size is 2% of the volume of the liquid medium;

[0060] In step 3), the mixed fermentation culture conditions: temperature 35° C.; 150 rpm; culture for 5 days.

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PUM

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Abstract

The invention discloses a composite microorganism bacterial agent for ginger. The composite microorganism bacterial agent for the ginger comprises a fermented liquid of the following strains: Bacillusjicheiformis SMrs15, Bacillus laterosporus LEs15, Bacillus megaterium FBs03, Bacillus subtilis FBrs14, Bacillus atrophaeus BHr4-8, Bacillus coagulans BHrs7-15 and Paenibacillus polymyxa SMr02; the total numbers of the communities of the Bacillus jicheiformis SMrs15, the Bacillus laterosporus LEs15, the Bacillus megaterium FBs03, the Bacillus subtilis FBrs14, the Bacillus atrophaeus BHr4-8, the Bacillus coagulans BHrs7-15 and the Paenibacillus polymyxa SMr02 in the fermented liquid are as follows in sequence: 8.0*10<8> to 1.5*10<10> cfu / mL, 8.0*10<8> to 0.5*10<10> cfu / mL, 8.0*10<8> to 1.5*10<10> cfu / mL, 8.0*10<8> to 1.5*10<10> cfu / mL, 8.0*10<8> to 1.5*10<10> cfu / mL, 8.0*10<8> to 1.5*10<10> cfu / mL, and 8.0*10<8> to 1.5*10<10> cfu / mL; the invention also discloses a preparation method of thecomposite microorganism bacterial agent. The composite microorganism bacterial agent disclosed by the invention is used for providing a special composite microorganism bacterial agent which meets thenutritional requirement on the growth of the ginger, so as to coordinate nutrients, promote the growth of the ginger, reduce successive cropping obstacles of the ginger, improve the quality, the yieldand the disease resistance and the stress resistance of the ginger, and achieve the purpose of improving soil characteristics.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a compound microbial agent for ginger, and also relates to a preparation method and application of the compound microbial agent. Background technique [0002] Ginger (Zingiber officinale Rosc.) is an important seasoning vegetable and nutritious vegetable in my country. It is the main economic crop in Shandong Province, and has gradually formed a large-scale and industrialized production. In recent years, ginger diseases have generally occurred, and the light ones have reduced production, and the severe ones have halved, or even lost production, which has seriously affected the production of ginger. In the continuous cropping of ginger, the more serious diseases are mainly ginger stem rot, spot disease and ginger blast. In recent years, with the improvement of ginger industrialization, the problem of continuous cropping has become more prominent. The occurren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P21/00A01P7/00C12R1/07C12R1/11C12R1/125C12R1/10C12R1/01
CPCA01N63/00C12N1/20C12R2001/10C12N1/205C12R2001/11C12R2001/125C12R2001/07C12R2001/01
Inventor 宿安祥秦波杨晓燕金辉牛艳丽辛爱一燕志强李秀壮
Owner 北大荒农服华农(北京)科技有限公司
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