Bismuth sulfide-zinc protoporphyrin composite material with tumor photodynamic therapy properties under near-infrared light excitation and its preparation method and application

A technology of photodynamic therapy and zinc protoporphyrin, which is applied in the field of composite materials and can solve the problems of low PDT efficiency

Active Publication Date: 2020-10-23
中科应化(长春)科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the technical problem of low PDT efficiency caused by the recombination of photogenerated electrons and holes in bismuth sulfide and the anti-oxidative stress ability of heme oxygenase-1 (HO-1) in cells in the prior art, and provides a A bismuth sulfide-zinc protoporphyrin composite material with tumor photodynamic therapy properties under near-infrared light excitation and its preparation method and application. The activity of heme oxygenase-1 in the body, thereby improving the efficiency of tumor photodynamic therapy

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  • Bismuth sulfide-zinc protoporphyrin composite material with tumor photodynamic therapy properties under near-infrared light excitation and its preparation method and application
  • Bismuth sulfide-zinc protoporphyrin composite material with tumor photodynamic therapy properties under near-infrared light excitation and its preparation method and application
  • Bismuth sulfide-zinc protoporphyrin composite material with tumor photodynamic therapy properties under near-infrared light excitation and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: the mass ratio is the synthesis of 99%: 1% bismuth sulfide-zinc protoporphyrin composite material:

[0066] (1) Weigh 20 mg of bismuth sulfide powder modified by poly-N-isopropylacrylamide-acrylamide copolymer, add it to 20 mL of ultrapure water, and ultrasonically disperse to obtain a bismuth sulfide dispersed aqueous solution;

[0067] (2) Take 1mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC, 5mg / mL) hydrochloride and add it to the above-mentioned bismuth sulfide dispersed aqueous solution, ultrasonically and stir at room temperature for 4 hours , dialysis to remove unreacted EDC, to obtain EDC-modified bismuth sulfide dispersion;

[0068] (3) Add 1 mL of N-hydroxysuccinimide (NHS, 5 mg / mL) ethanol to the above-mentioned EDC-modified bismuth sulfide dispersion, ultrasonically stir at room temperature for 4 hours, dialyze to remove unreacted NHS, and obtain NHS-modified bismuth sulfide dispersion;

[0069] (4) Take 1 mL of zinc amidated protop...

Embodiment 2

[0070] Embodiment 2: mass ratio is the synthesis of 99.5%: 0.5% bismuth sulfide-zinc protoporphyrin composite material:

[0071] (1) Weigh 20 mg of bismuth sulfide powder modified by poly-N-isopropylacrylamide-acrylamide copolymer, add it to 20 mL of ultrapure water, and ultrasonically disperse to obtain a bismuth sulfide dispersed aqueous solution;

[0072] (2) Take 1mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC, 5mg / mL) hydrochloride and add it to the above-mentioned bismuth sulfide dispersion, ultrasonically stir at room temperature for 4 hours , centrifuging to remove unreacted EDC to obtain a bismuth sulfide dispersion modified by EDC;

[0073] (3) Add 1 mL of N-hydroxysuccinimide (NHS, 5 mg / mL) ethanol to the above-mentioned EDC-modified bismuth sulfide dispersion, ultrasonically stir at room temperature for 4 hours, and centrifuge to remove unreacted NHS to obtain NHS-modified bismuth sulfide dispersion;

[0074] (4) Add 0.8 mL of zinc amidated protoporphyr...

Embodiment 3

[0075] Embodiment 3: mass ratio is the synthesis of 98%:2% bismuth sulfide-zinc protoporphyrin composite material:

[0076] (1) Weighing 15 mg of bismuth sulfide powder modified by poly-N-isopropylacrylamide-acrylamide copolymer was added to 20 mL of ultrapure water, and ultrasonically dispersed to obtain a bismuth sulfide dispersed aqueous solution;

[0077] (2) Take 1mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC, 5mg / mL) hydrochloride and add it to the above-mentioned bismuth sulfide dispersion, ultrasonically stir at room temperature for 2 hours , dialysis to remove unreacted EDC, to obtain EDC-modified bismuth sulfide dispersion;

[0078] (3) Add 1 mL of N-hydroxysuccinimide (NHS, 5 mg / mL) ethanol to the above-mentioned EDC-modified bismuth sulfide dispersion, ultrasonically stir at room temperature for 2 hours, dialyze to remove unreacted NHS, and obtain NHS-modified bismuth sulfide dispersion;

[0079] (4) Add 0.5mL zinc amidated protoporphyrin (500μM) to the ...

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Abstract

The invention relates to a bismuth sulfide-zinc protoporphyrin composite material with a tumor photodynamic therapy (PDT) property under excitation of near-infrared light as well as a preparation method and application and belongs to the field of composite materials, aiming at solving the problem that the PDT efficiency is relatively low, caused by photoelectron-hole compounding in a photodynamictherapy process of bismuth sulfide and an anti-oxidization stress capability of heme oxygenase (HO-1) in cells. The composite material is the bismuth sulfide-zinc protoporphyrin composite material which is obtained by synthesizing a polyN-isopropylacrylamide-acrylamide copolymer modified bismuth sulfide nano-material and then carrying out condensation reaction of carboxyl and amino. The prepared composite material has the effect of enhancing the photodynamic therapy efficiency through two ways of inhibiting the activity of the heme oxygenase and promoting electron-hole separation; meanwhile, the material provided by the invention has good biocompatibility, light stability, CT (Computed Tomography) imaging capability and enhanced PDT efficiency; a thought is provided for designing a novel nano diagnosis and treatment integrated system and the material has important meaning on early-stage diagnosis and treatment of cancers.

Description

technical field [0001] The invention belongs to the technical field of composite materials, and in particular relates to a bismuth sulfide-zinc protoporphyrin composite material with tumor photodynamic therapy properties under near-infrared light excitation, a preparation method and an application. Background technique [0002] Photodynamic therapy (PDT), as a non-invasive medical technology, has the advantages of low toxicity, small side effects, broad-spectrum anti-cancer, and high targeting compared with conventional treatments such as surgery, chemotherapy, and radiotherapy. Compared with gold and tantalum, bismuth has the highest relative atomic number (tantalum, 73; gold, 79; bismuth, 83) and X-ray attenuation coefficient (at 100keV accelerating voltage: tantalum, 4.30; gold, 5.16; bismuth, 5.74cm 2 / g), significantly greater than iodine (53 and 1.94cm 2 / g), so bismuth can be used as X-ray computerized tomography (Computed Tomography, CT) contrast agent, and the con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/58A61K49/04A61P35/00
CPCA61K41/0057A61K41/0071A61K49/04
Inventor 程岩张海元冯艳林常赟孙秀娟菅慧
Owner 中科应化(长春)科技有限公司
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