Method for producing high-quality extract by rapidly fermenting ginkgo leaf powder

Abstract

The invention discloses a method for producing a high-quality extract by rapidly fermenting ginkgo leaf powder. A strain used for fermentation is trichoderma reesei. The method comprises the steps that dried ginkgo leaves are smashed to 20-40 mesh and soaked in deionized water according to the material-to-liquid ratio of 1:5-1:8 to adjust the pH value to be 3-6, the mixture is sucked into a fermentation tank to be sterilized for 3-10 min at 116-125 DEG C and then cooled to 25-30 DEG C, a trichoderma reesei seed solution with the mass accounting for 1-10% of the dry weight of the ginkgo leaf powder in fermentation broth is added, aerated fermentation is conducted for 2-10 days at 25-30 DEG C, and the rotation speed during stirring is 180-300 r / min; after fermentation, a 20-95% ethanol solution is used for reflux extraction for 1-3 h for 2-3 times according to the material-to-liquid ratio of 1:7-1:12, extraction solutions are combined, filtered and subjected to vacuum concentration, thenthe product is purified by using LX-5 macroporous adsorption resin, the product is washed with deionized water and a 10-20% ethanol solution in sequence and finally eluted by using a 65-85% ethanol solution, and eluant is subjected to vacuum concentration to be free of alcohol smell; after filtering is completed, the concentration of the product is adjusted to be proper with deionized water, thenginkgolic acid is removed by using LXD-200 macroporous adsorption resin, and a deacidification extraction solution is subjected to concentration, drying and smashing to obtain the ginkgo leaf extract. The ginkgo leaf extract prepared through the method is high in extraction efficiency, good in product quality and suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for rapidly fermenting ginkgo leaf powder to produce high-quality extracts. Background technique [0002] Ginko Biloba Extract (GBE) is a light brown-yellow flowable powder extracted from the dried stems and leaves of Ginkgo biloba, which mainly contains flavonoids and diterpene lactones. Expand cardiovascular and cerebrovascular, improve microcirculation, inhibit platelet aggregation, anti-oxidation, scavenge and inhibit oxygen free radicals, delay aging, anti-virus, anti-bacterial and anti-inflammatory, regulate endocrine and other physiological functions. [0003] The content of flavonoids in Ginkgo biloba is about 6%, including flavonoids, flavonols, dihydroflavones and their glycosides, the main components of which are quercetin, kaempferol and isorhamnetin; lactones mainly include Ginkgolide A, Ginkgolide B, Ginkgolide C and Bilobalide. The current inte...

AI Technical Summary

Problems solved by technology

[0004] The existing production methods of Ginkgo biloba extract include water extraction, organic solvent extraction, supercritical fluid extraction, ultrasonic-assisted extraction, etc., but they all have defects such as low extraction efficiency and high cost.

Flavonoids and lactones in Ginkgo biloba are wrapped in the cell wall (connected by β-D glucose with 1,4-β glycosidic bonds). Most of the traditional methods cannot completely break the cell wall, which limits the dissolution of flavonoids and seriously affects extraction efficiency