Method for producing high-quality extract by rapidly fermenting ginkgo leaf powder

A high-quality technology of ginkgo biloba powder, applied in the directions of medical raw materials, pharmaceutical formulations, plant raw materials, etc. derived from Ginkgo biloba Compare and improve the effect of product quality

Active Publication Date: 2018-06-22
SHAANXI SCI TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing production methods of Ginkgo biloba extract include water extraction, organic solvent extraction, supercritical fluid extraction, ultrasonic-assisted extraction, etc., but they all have defects such as low extraction efficiency and high cost.
Flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] (1) Take 50.0g of dried Ginkgo biloba powder from 20-40 mesh, dilute with water at a material-to-liquid ratio of 1:5 (m / v), stir well, adjust the pH of the solution to 3.0 with 2mol / L hydrochloric acid, and fill it into a 500mL triangle Sterilize the bottle in an autoclave at 116°C for 10 minutes.

[0032] (2) After the sterilization, the temperature was lowered to 25°C, and 1 mL of Trichoderma reesei seed liquid was added at a 2% inoculum amount in a sterile environment, and fermented on a constant temperature shaker at 25°C for 10 days at a speed of 180r / min.

[0033] (3) After the fermentation, add 200mL 95% ethanol solution for reflux extraction, the extraction temperature is 40℃, the extraction time is 3h, after the extraction, filter, add 500mL 20% ethanol to the filter residue and extract once according to the above conditions, filter, combine the filtrate twice, and concentrate To 300mL.

[0034] (4) Purify the extract with LX-5 macroporous adsorptio...

Example Embodiment

[0037] Example 2

[0038] (1) Take 100.0g of dried Ginkgo biloba powder from 20-40 mesh, dilute with water at a material-to-liquid ratio of 1:6 (m / v), stir well, adjust the pH of the solution to 4.0 with 2mol / L hydrochloric acid, and divide into 4 In a 500mL Erlenmeyer flask, sterilize in a high-pressure steam sterilizer at 118°C for 8 minutes.

[0039] (2) After the sterilization, the temperature was lowered to 27°C, and 1 mL of Trichoderma reesei seed liquid was added to each bottle at a 4% inoculum amount in a sterile environment. Fermentation was carried out on a constant temperature shaker at 27°C for 8 days at a speed of 220r / min.

[0040] (3) After the fermentation, add 250mL of 95% ethanol solution to each bottle for reflux extraction. The extraction temperature is 55℃, and the extraction time is 2h. After the extraction is completed, filter, add 800mL of 40% ethanol to the filter residue and extract twice under the above conditions, filter, and combine the three filtrates. ...

Example Embodiment

[0044] Example 3

[0045] (1) Take 200.0g of dried ginkgo biloba powder with 20-40 mesh, dilute with water at a material-to-liquid ratio of 1:7 (m / v), stir well, adjust the pH of the solution to 5.0 with 2mol / L acetic acid, and divide into 7 Sterilize in a 1000mL Erlenmeyer flask at 121°C for 6 min in an autoclave.

[0046] (2) After the sterilization, the temperature was lowered to 28°C, and 1.7 mL of Trichoderma reesei seed liquid was added to each bottle at a 6% inoculum amount in a sterile environment, and fermented on a constant temperature shaker at 28°C for 6 days at a speed of 250r / min.

[0047] (3) After the fermentation, add 300mL of 95% ethanol solution to each bottle for reflux extraction. The extraction temperature is 70℃, the extraction time is 1.5h. After the extraction is completed, filter, add 2000mL of 60% ethanol to the filter residue and extract 2 times under the above conditions, filter, and combine 3 times The filtrate was concentrated to 2000mL.

[0048] (4) Pu...

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PUM

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Abstract

The invention discloses a method for producing a high-quality extract by rapidly fermenting ginkgo leaf powder. A strain used for fermentation is trichoderma reesei. The method comprises the steps that dried ginkgo leaves are smashed to 20-40 mesh and soaked in deionized water according to the material-to-liquid ratio of 1:5-1:8 to adjust the pH value to be 3-6, the mixture is sucked into a fermentation tank to be sterilized for 3-10 min at 116-125 DEG C and then cooled to 25-30 DEG C, a trichoderma reesei seed solution with the mass accounting for 1-10% of the dry weight of the ginkgo leaf powder in fermentation broth is added, aerated fermentation is conducted for 2-10 days at 25-30 DEG C, and the rotation speed during stirring is 180-300 r/min; after fermentation, a 20-95% ethanol solution is used for reflux extraction for 1-3 h for 2-3 times according to the material-to-liquid ratio of 1:7-1:12, extraction solutions are combined, filtered and subjected to vacuum concentration, thenthe product is purified by using LX-5 macroporous adsorption resin, the product is washed with deionized water and a 10-20% ethanol solution in sequence and finally eluted by using a 65-85% ethanol solution, and eluant is subjected to vacuum concentration to be free of alcohol smell; after filtering is completed, the concentration of the product is adjusted to be proper with deionized water, thenginkgolic acid is removed by using LXD-200 macroporous adsorption resin, and a deacidification extraction solution is subjected to concentration, drying and smashing to obtain the ginkgo leaf extract. The ginkgo leaf extract prepared through the method is high in extraction efficiency, good in product quality and suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for rapidly fermenting ginkgo leaf powder to produce high-quality extracts. Background technique [0002] Ginko Biloba Extract (GBE) is a light brown-yellow flowable powder extracted from the dried stems and leaves of Ginkgo biloba, which mainly contains flavonoids and diterpene lactones. Expand cardiovascular and cerebrovascular, improve microcirculation, inhibit platelet aggregation, anti-oxidation, scavenge and inhibit oxygen free radicals, delay aging, anti-virus, anti-bacterial and anti-inflammatory, regulate endocrine and other physiological functions. [0003] The content of flavonoids in Ginkgo biloba is about 6%, including flavonoids, flavonols, dihydroflavones and their glycosides, the main components of which are quercetin, kaempferol and isorhamnetin; lactones mainly include Ginkgolide A, Ginkgolide B, Ginkgolide C and Bilobalide. The current inte...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/19A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 冯自立赵正栋张晓娟张辰露陈旺屈义虎
Owner SHAANXI SCI TECH UNIV
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