Method for producing high-quality extract by rapidly fermenting ginkgo leaf powder
A high-quality technology of ginkgo biloba powder, applied in the directions of medical raw materials, pharmaceutical formulations, plant raw materials, etc. derived from Ginkgo biloba Compare and improve the effect of product quality
Active Publication Date: 2018-06-22
SHAANXI SCI TECH UNIV
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Abstract
The invention discloses a method for producing a high-quality extract by rapidly fermenting ginkgo leaf powder. A strain used for fermentation is trichoderma reesei. The method comprises the steps that dried ginkgo leaves are smashed to 20-40 mesh and soaked in deionized water according to the material-to-liquid ratio of 1:5-1:8 to adjust the pH value to be 3-6, the mixture is sucked into a fermentation tank to be sterilized for 3-10 min at 116-125 DEG C and then cooled to 25-30 DEG C, a trichoderma reesei seed solution with the mass accounting for 1-10% of the dry weight of the ginkgo leaf powder in fermentation broth is added, aerated fermentation is conducted for 2-10 days at 25-30 DEG C, and the rotation speed during stirring is 180-300 r/min; after fermentation, a 20-95% ethanol solution is used for reflux extraction for 1-3 h for 2-3 times according to the material-to-liquid ratio of 1:7-1:12, extraction solutions are combined, filtered and subjected to vacuum concentration, thenthe product is purified by using LX-5 macroporous adsorption resin, the product is washed with deionized water and a 10-20% ethanol solution in sequence and finally eluted by using a 65-85% ethanol solution, and eluant is subjected to vacuum concentration to be free of alcohol smell; after filtering is completed, the concentration of the product is adjusted to be proper with deionized water, thenginkgolic acid is removed by using LXD-200 macroporous adsorption resin, and a deacidification extraction solution is subjected to concentration, drying and smashing to obtain the ginkgo leaf extract. The ginkgo leaf extract prepared through the method is high in extraction efficiency, good in product quality and suitable for industrial large-scale production.
Application Domain
Ginkgophyta medical ingredientsPlant ingredients
Technology Topic
Fermentation brothReflux extraction +6
Examples
- Experimental program(4)
- Effect test(1)
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