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A kind of anaerobic cultivation device and its application

An anaerobic culture and culture medium technology, applied in the field of microbial preparation, can solve the problems of not being able to perform parallel experiments at the same time, affecting the repeatability of experimental results, and limiting the applicability of the device, achieving strong adaptability, wide use and reliable results. Effect

Active Publication Date: 2021-01-15
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The existing cultivation method may not be accurate and convenient because the parameters of the control device cannot be digitally recorded and adjusted in real time; or it cannot be modularized due to centralized control, resulting in limited applicability of the device and low design customization. The operation is complicated when conducting different experiments; or only a simple series design is used, so that parallel experiments cannot be carried out at the same time during continuous fermentation, which makes the test cycle longer and affects the repeatability of the experimental results

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  • A kind of anaerobic cultivation device and its application
  • A kind of anaerobic cultivation device and its application
  • A kind of anaerobic cultivation device and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] An anaerobic cultivation device of the present embodiment comprises an anaerobic system, a fermentation system and a control system 5; the anaerobic system and the fermentation system are connected by pipelines to form an anaerobic gas cycle, and the control system 5 uses Used to control the pH and temperature conditions of the fermentation system;

[0036] The anaerobic system includes a nitrogen bottle 1 and a gas collection bag, the nitrogen bottle 1 is used to feed nitrogen into the fermentation system to create an anaerobic environment, and the gas collection bag is used to collect the gas produced during the fermentation process;

[0037]The fermentation system comprises a seed tank 31, a fermenter group 32 and a feeding bottle group 2 connected by pipelines, the seed tank 31 and the fermenter group 32 are connected in series, and the feeding bottle group 2 is connected with the seed tank 31, the fermentation tank group 2 The tank group 32 is connected, and is use...

Embodiment 2

[0049] Such as figure 1 As shown, the method for carrying out the multi-stage continuous culture of human intestinal flora by using the above-mentioned device comprises the following steps:

[0050] (1) Prepare a healthy human intestinal flora suspension, and preserve the species in the seed tank 31;

[0051] (2) Establish a culture model: the fermenter group 32 is set to two groups, including two groups connected in parallel, each group comprising a first fermenter and a second fermenter connected in series, and the first fermenter is used for Simulating the environment of the proximal colon of the human body, the second fermenter is used to simulate the environment of the distal colon of the human body;

[0052] figure 1 Among them, B1 is a medium feeding bottle, B2, B3, B4, B5, and B6 are lye feeding bottles, F1 is a seed tank 31, and F2, F3, F4, and F5 are fermentation tanks. The volume of the fermented liquid in the first fermentors F2 and F4 used to simulate the proxi...

Embodiment 3

[0058] The method for batch culture using the above-mentioned device is used to evaluate the effect of prebiotics on human intestinal flora, including the following steps:

[0059] (1) Preparation of healthy human intestinal flora suspension

[0060] Fecal samples were obtained from fecal bacteria transplantation donors in Zhongshan Hospital Affiliated to Xiamen University. After the fecal samples were processed by the "fecal bacteria intelligent separation system", 30 g of the separated bacteria were taken and resuspended in 80 mL of normal saline for inoculation.

[0061] (2) Preparation of medium for fermentation

[0062] Such as figure 2 As shown, the modules in the anaerobic culture device are assembled and used for batch fermentation. Both fermenters use basal nutrient medium, one of which adds fructo-oligosaccharides to the experimental group, and the other to the control group without adding fructooligosaccharides. Fructose. The air outlet of the bottle cap d of th...

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Abstract

The invention discloses an anaerobic culture device and application thereof. The anaerobic culture device comprises an anaerobic system, a fermentation system and a control system, wherein the anaerobic system and the fermentation system are connected through a pipeline so as to form anaerobic gas circulation; the control system is capable of realizing independent control on PH (Potential of Hydrogen) and temperature conditions of each tank body in a grouping way. Grouped modular assembly is realized, the operation is convenient, an experiment process is high in repeatability, an application range is wide, and the anaerobic culture device is particularly suitable for culture of in-vitro intestinal flora and metabolism evolution research of anaerobic bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganism preparation, and in particular relates to an anaerobic culture device and its application. Background technique [0002] The Human Microbiome Project initiated by the US NIH between 2008 and 2013 and the Human Gut Metagenome Project initiated by the European Union between 2008 and 2012, as well as a large number of related studies in recent years have shown that human The number of symbiotic microorganisms is huge and has a huge impact on human health, and the impact of intestinal microorganisms is the most core and comprehensive. Relevant studies have reported that gut microbes are associated with human immunity and most chronic diseases. While the structure of intestinal microecological flora has a major impact on the health of the host, diseases, food, and drugs also have an impact on the intestinal flora itself. Based on this, it is necessary to study the interaction between intestinal...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12N1/20C12R1/145
CPCC12M23/58C12M29/00C12M41/12C12M41/18C12M41/26C12M41/48C12N1/20C12M29/06C12M41/22
Inventor 方柏山张俊楠付友思
Owner XIAMEN UNIV
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