Bioremediation reagent for promoting hyperaccumulator growth and enhancing soil heavy metal extraction and remediation method

A bioremediation and plant growth technology, which is applied in the field of agriculture and environmental pollution control, can solve problems such as single method and insignificant effect, and achieve the effects of promoting growth, enhancing extraction effect, and strong growth-promoting effect

Active Publication Date: 2018-07-10
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only relying on phytoremediation or microbial remediation is too simple, the effect is

Method used

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  • Bioremediation reagent for promoting hyperaccumulator growth and enhancing soil heavy metal extraction and remediation method
  • Bioremediation reagent for promoting hyperaccumulator growth and enhancing soil heavy metal extraction and remediation method
  • Bioremediation reagent for promoting hyperaccumulator growth and enhancing soil heavy metal extraction and remediation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening, identification and biological characteristics of embodiment 1 joint bacillus PGP41 (the results are shown in Table 1, Table 2, figure 1 )

[0025] 1) Source of soil samples: plant rhizosphere soil of the lead-zinc mine in Fangxian County, Hubei Province. Collector: Zhang Zhou, contact number: 025-84396391, collection unit: Nanjing Agricultural University.

[0026] 2) Screening of bacterial strains: Take 10 g of collected plant root soil samples, put them into an Erlenmeyer flask containing 90 mL of sterile water, vibrate in a shaker for 20 min, and let stand for 20-30 s to make a bacterial suspension. Under sterile conditions, take 1mL of bacterial suspension and put it into a 9mL sterile water test tube, mix well, and dilute with sterile water to make 10 -2 、10 -3 、10 -4 、10 -5 , 10 -6 Diluents of various concentrations.

[0027] Take 0.1 mL of the dilutions of the above concentrations and spread them on the enrichment medium respectively, and set thr...

Embodiment 2

[0054] Embodiment 2 liquid preparation

[0055] Insert Arthrobacter PGP41 into LB medium for activation for 18-20h, insert 5-10% (v / v) of the inoculum into LB medium at 30°C, and culture on a shaker at 150-200rmp / min for 20h-24h to become a fermented seed liquid. Liquid seed LB medium: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH=7.0.

[0056] Fermentation tank fermentation:

[0057] Fermentation tank medium formula: tryptone 5-10%, yeast extract 5-10%, sodium chloride 5-10%, the rest is water, the pH value before disinfection is 7.0-7.8, and the pH after disinfection is 6.8-7.5. The percentage content of the medium formula composition is all percentage by weight; The seed liquid obtained in the previous step is inoculated into the fermenter with an inoculation amount of 5%-10% of the volume percentage of the culture medium to be inoculated, feeds sterile air and stirs, at 28- Cultivate at 35°C for 24-36 hours, with an aeration rate of 1-2 Vols / vol·min and a...

Embodiment 3

[0059] Embodiment 3 solid preparation

[0060] The culture solution obtained in the example was centrifuged at 8000rpm for 15-20min to obtain pure PGP41 bacteria, and a protective agent (4.51mg / g sucrose, 0.9mg / g trehalose, 9.6mg / g glucose) was added to it. , the solid bacterial agent powder can be made by freeze-drying method. 1.9951 g of solid bacterial powder of PGP41 cells can be prepared per liter of fermentation broth. After testing, the effective number of viable bacteria in the solid bioremediation preparation was 3.329×10 11 pcs / g.

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Abstract

The invention relates to a bioremediation reagent for promoting hyperaccumulator growth and enhancing soil heavy metal extraction and a remediation method. Firstly, a hyperaccumulator is planted in in-situ contaminated soil, and plant growth promoting bacteria are inoculated to the plant rhizosphere, conventional culture, watering and moisturizing are carried out, the growth promoting characteristics of the plant growth promoting bacteria are utilized to promote plant growth and enhance the plant's efficient extraction of heavy metals from the contaminated soil, respective advantages of the plant and microbial remediation are brought into full play to draw on each other's strength, and can improve the disadvantages of low remediation efficiency, small plant biomass, long remediation periodand the like in single plant remediation, thus improving the phytoremediation efficiency, and achieving the purpose of complete remediation of soil heavy metal pollution.

Description

technical field [0001] The invention belongs to the technical field of agriculture and environmental pollution control, and relates to a bioremediation reagent for promoting the growth of hyperaccumulative plants and enhancing the extraction of heavy metals in soil, and a combined plant-microbe restoration method for promoting the growth of hyperaccumulative plants and extracting heavy metals from complex polluted soil. Background technique [0002] With the rapid development of industrial and agricultural production and the continuous acceleration of urbanization, soil heavy metal pollution is becoming more and more serious. The main pollution sources of heavy metals in farmland in my country are sewage irrigation, application of pesticides and fertilizers, accumulation of solid waste, mining and smelting, etc. Heavy metal pollution has a great impact on the production, yield and quality of crops, especially after being absorbed and enriched by crops, it can endanger human ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/06
CPCB09C1/10B09C1/105B09C2101/00C12N1/205C12R2001/06
Inventor 陈晨许梦陈亚华沈振国
Owner NANJING AGRICULTURAL UNIVERSITY
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