Method for detecting nitrate nitrogen stable isotope in water

A technology of stable isotope and nitrate nitrogen, which is applied in the field of determination of stable isotope of nitrate nitrogen in water body, can solve the problems of unfavorable environment, long cultivation cycle of denitrifying bacteria, cumbersome operation, etc., and achieve the safety of operators, accurate and reliable measurement results, The effect of easy operation

Active Publication Date: 2018-07-10
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

The chemical method is to use copper-plated cadmium particles to convert nitrate into nitrite, and then use azide acid to convert nitrite into N 2 O, azido acid is a highly toxic substance and has the risk of explosion. The heavy metal waste liquid after copper-plated cadmium grain treatment is not good for the environment
Bacterial denitrification is the use of the lack of nitric acid N 2 O-reductase denitrifying bacteria convert nitrate to N 2 O, the advantage of this method is that it is safe to operate, and the measurement result is more accurate than the chemical method. The disadvantage is that denitrifying bacteria need to be cultivated for a long period, and the operation is cumbersome.

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  • Method for detecting nitrate nitrogen stable isotope in water
  • Method for detecting nitrate nitrogen stable isotope in water

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Embodiment 1

[0023] The present embodiment provides a kind of assay method of nitrogen stable isotope of nitrate in water, and the reagent and material involved mainly include: Potassium nitrate (precisely calibrated δ with elemental analyzer-stable isotope mass spectrometer) 15 N value is 5.35‰), benzene, concentrated sulfuric acid, nitrate isotope standard materials (USGS32, USGS34, USGS35).

[0024] The method is specifically: the known δ 15 Potassium nitrate with an N value is prepared into a potassium nitrate solution with a concentration of 100 mg / L as the water body to be tested. Take 5 mL of the water body to be tested, pass through a 0.45 μm microporous membrane, add 1 times the volume of benzene and mix it in the resulting mixed solution. Slowly add 1.5 times the volume of concentrated sulfuric acid dropwise along the container wall, oscillate and mix for 2 minutes, then let the layers stand for 15 minutes, take the upper layer solution, remove water through anhydrous sodium sulf...

Embodiment 2

[0034] Compared with Example 1, the difference is only: the water body to be tested is 2 parts of environmental water bodies; the method described in Example 1 is used to detect the δ of 2 parts of environmental water bodies 15 The N values ​​are 2.11±0.26‰ and 7.21±0.13‰, respectively.

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Abstract

The invention relates to a method for detecting a nitrate nitrogen stable isotope in water. The method comprises the following steps: mixing water to be detected with benzene, slowly adding concentrated sulfuric acid to the obtained mixed solution, performing oscillation to achieve uniform mixing, standing the obtained solution to achieve layering, taking the obtained upper layer solution, converting nitrates in the water to be detected into nitrobenzene, and detecting the nitrogen stable isotope in the converted water. The method only uses concentrated sulfuric acid and benzene solvents, anddetects the nitrobenzene monomer nitrogen isotope through derivatizing the nitrates into nitrobenzene and using GC-C-IRMS. Compared with traditional methods, the method in the invention has the advantages of simplicity in operation, short time, high efficiency, safety in operation, accuracy and reliability in a detection result, and has reference meaning in the detection of the nitrate nitrogen stable isotope in other environment samples.

Description

technical field [0001] The invention belongs to the field of traceability and detection of environmental pollutants, in particular to a method for measuring stable isotopes of nitrate nitrogen in water bodies. Background technique [0002] Nitrate is a major pollutant in surface water and shallow groundwater. Excessive nitrate content in surface water will lead to eutrophication of the water body. Excessive nitrate content in irrigation water will affect the quality of crop products. Excessive nitrate content in drinking water may cause harm through conversion into nitrosamine carcinogens. human health. There are many sources of nitrate pollution, such as domestic sewage discharge, industrial sewage discharge, abuse of nitrogen fertilizer, dry and wet deposition of atmospheric nitrogen oxides, etc. Research to clarify the source of nitrate pollution in water is of great significance for controlling nitrate pollution in water, protecting the ecological environment and human...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 李安潘立刚董洁琼靳欣欣付海龙
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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