Chromogenic medium and detection sheet capable of simultaneously detecting coliform and salmonella

Abstract

The invention discloses a chromogenic medium and a chromogenic detection sheet capable of simultaneously detecting coliform and salmonella. The chromogenic medium contains peptone, yeast extract powder, beef extract powder, sodium chloride, sodium pyruvate, cholate, a specific enzyme chromogenic substrate, an enzyme inducer, a stabilizer, novobiocin and high molecular water-absorbent gel. The chromogenic medium is used for simultaneously detecting the coliform and the salmonella, is high in detection sensitivity, short in detection time and high in efficiency, and can simultaneously perform qualitative detection and quantitative detection and analysis on the coliforms and the salmonella, so that experimental operation is significantly reduced and the cost is saved; the chromogenic medium can be applied to direct sampling detection on tableware, food producing environment and equipment detection and other places; the coliform shows blue bacterial spots, and the salmonella shows red bacterial spots.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a chromogenic medium and a detection sheet for simultaneously detecting coliforms and salmonella. Background technique [0002] Coliform bacteria are a class of aerobic and facultative anaerobic Gram-negative non-bacillus bacteria that can ferment lactose to produce acid and gas under certain culture conditions. Scientific research reports show that coliform bacteria are widely present in animal feces, water bodies polluted by them, food production lines and other environments. Coliform bacteria has been used as one of the important indicators of the hygienic quality of food and its contact medium. [0003] Salmonella is a member of the Enterobacteriaceae family and is an important pathogenic microorganism that can cause zoonotic diseases. Food poisoning cases caused by eating food contaminated by Salmonella have been reported in the newspapers, so they are subject to food...

AI Technical Summary

Problems solved by technology

[0008] The existing chromogenic medium relies on large doses of high-selectivity bacteriostatic agents as bacteria screening conditions. The medium cannot be sterilized at high temperature and may cause false positives due to bacteria in the medium itself. Similar coliform chromogenic medium and Salmonella show Chromochrome medium and its rapid detection paper discs and other similar products cannot achieve the purpose of simultaneous detection due to the mutual inhibition of selective substances or the lack of corresponding specific enzyme chromogenic substrates and enzyme inducers

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