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Method for preparing bacterial cellulose from tofu yellow seriflux

A technology of bacterial cellulose and yellow pulp water, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution and resource waste, reduce enterprise investment, reduce enterprise production costs, and alleviate pollution effect

Inactive Publication Date: 2018-07-24
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yellow pulp water is directly discharged without any treatment, which not only causes environmental pollution, but also causes waste of resources

Method used

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  • Method for preparing bacterial cellulose from tofu yellow seriflux

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Collect the yellow pulp water produced in the tofu production process, pasteurize it after adding 6% sucrose by mass fraction; insert freshly activated Acetobacter xylinus (Gluconacetobacter xylinus, the strains were purchased from China General Microorganism Culture Preservation Management Co., Ltd. Center, the strain preservation number is CGMCC 1.1812) up to 10 8 CFU / mL yellow pulp water seed liquid, fermented at 30°C for 3 days to obtain bacterial cellulose; take out the bacterial cellulose, leave 30% of the fermentation liquid in the fermentation device, add 3 times the volume of the fermentation liquid after pasteurization The sugary yellow pulp water is used for the next round of bacterial cellulose production.

[0023] The preparation method of black tea and green tea sugar water in Table 1 is to add 0.5% black tea or green tea and 6% sucrose to the water, boil for 5 minutes, and filter the tea leaves with gauze after cooling. The total amount of Acetobacter xy...

Embodiment 2

[0028] Collect the yellow slurry water produced in the tofu production process, add mass fractions of 1% fructose, 1% maltose, and 4% sucrose and pasteurize it; the ratio of the number of freshly activated bacteria is 10:3:5 Acetobacter xylinus (Gluconacetobacter xylinus, strains purchased from China General Microorganism Culture Collection Management Center, strain preservation number is CGMCC 1.1812), Gluconacetobacter sp. (Gluconacetobacter sp., purchased from China Industrial Microbiology Culture Collection Management Center , the strain preservation number is CICC 10773) and Acetobacter pasteurianus (Acetobacter pasteurianus, the strain was purchased from the China General Microorganism Culture Collection Management Center, the strain preservation number is CGMCC 1.2269), and the total number of colonies reached 10 8 CFU / mL of the yellow pulp water seed solution was fermented at 28°C for 3 days to obtain bacterial cellulose; the bacterial cellulose was taken out, and 20% o...

Embodiment 3

[0030] Collect the yellow pulp water produced in the tofu production process, add mass fractions of 1% fructose, 1% sucrose, 1% maltose and 1% glucose and pasteurize it; the ratio of the number of freshly activated bacteria is 10: 2:3:5 Gluconacetobacter xylinus (Gluconacetobacter xylinus, strains purchased from China General Microorganism Culture Collection Management Center, strain preservation number is CGMCC 1.1812), Gluconacetobacter sp. (Gluconacetobacter sp., purchased from China Industrial Microbiology Culture Collection Management Center, the strain preservation number is CICC 10773), Saccharomyces cerevisiae (strains were purchased from the China General Microorganism Culture Collection Management Center, strain preservation number is CGMCC 2.3888), Debariae hansenii ( Debaryomyces hansenii, the strains were purchased from China General Microorganism Culture Collection Management Center, the strain preservation number is CGMCC 2.3948), and the total number of colonies...

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Abstract

The invention discloses a method for preparing bacterial cellulose from tofu yellow seriflux. The method comprises the steps: collecting yellow seriflux generated in a tofu producing process, adding sugar, sterilizing and accessing acetic acid bacteria or acetic acid bacteria, saccharomycetes and lactic acid bacteria mixed bacteria or kombucha seed solution or mycoderm capable of generating bacterial cellulose to ferment to obtain bacterial cellulose. According to the method disclosed by the invention, waste-yellow seriflux generated in tofu production is fully utilized as a raw material to produce the bacterial cellulose; not only is waste turned into wealth, but also enterprise profit is improved; furthermore, the bacterial cellulose has the advantages of high yield, strong elasticity and water retention, simple technology, low equipment requirement, favorability for large-scale popularization and wide market prospect.

Description

technical field [0001] The invention relates to a method for preparing bacterial cellulose by using tofu yellow slurry water, and belongs to the field of high-value utilization of processing by-products. Background technique [0002] Bacterial Cellulose (BC for short) is a kind of natural cellulose mainly produced by bacterial fermentation. It has the same chemical composition as plant cellulose, but its morphological structure and supramolecular structure are different from plant cellulose, and it belongs to a typical biological nanofiber material. [0003] Compared with plant cellulose, BC has more superior characteristics. The main component of plant cellulose is cellulose, in addition to cellulose, it also contains hemicellulose and lignin, the three exist in the form of three-dimensional structure, and BC itself is pure cellulose, so BC has high purity and high The characteristics of crystallinity; the elasticity of BC is about 10 times that of ordinary plant cellulos...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/01C12R1/02C12R1/645C12R1/865C12R1/23C12R1/72C12R1/74C12R1/225
CPCC12P19/04C12P39/00
Inventor 夏秀东周剑忠单成俊刘小莉王英李莹张丽霞张宏志
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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