Method for preparing bacterial cellulose from tofu yellow seriflux
A technology of bacterial cellulose and yellow pulp water, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution and resource waste, reduce enterprise investment, reduce enterprise production costs, and alleviate pollution effect
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[0021] Example 1
[0022] Collect the yellow syrup produced in the production process of tofu, add 6% sucrose and pasteurize it; connect to the freshly activated Gluconacetobacter xylinus (Gluconacetobacter xylinus, strain purchased from China's common microbial strain preservation management Center, the bacterial preservation number is CGMCC 1.1812) reached 10 8 The seed liquid of CFU / mL yellow pulp water is fermented at 30°C for 3 days to obtain bacterial cellulose; the bacterial cellulose is taken out, and the remaining 30% fermentation broth is placed in the fermentation device, and 3 times the volume of the fermentation broth is added after pasteurization The sugar-containing yellow syrup water is used for the next round of bacterial cellulose production.
[0023] The black tea and green tea syrup preparation method in Table 1 is obtained by adding 0.5% black tea or green tea and 6% sucrose to the water, boiling for 5 minutes, and filtering the tea leaves with gauze after cool...
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[0027] Example 2
[0028] Collect the yellow syrup produced during the production of tofu, add 1% fructose, 1% maltose, and 4% sucrose and pasteurize it; the ratio of the number of freshly activated bacteria is 10:3:5 Gluconacetobacter xylinus (Gluconacetobacter xylinus, purchased from China General Microbial Culture Collection and Management Center, the collection number is CGMCC 1.1812), Gluconacetobacter sp., purchased from China Industrial Microbial Culture Collection and Management Center , The preservation number of the strain is CICC 10773) and Acetobacter pasteurianus (Acetobacter pasteurianus, the strain was purchased from the China General Microbial Culture Collection and Management Center, the strain preservation number is CGMCC 1.2269), the total number of colonies reached 10 8 CFU / mL yellow pulp water seed liquid is fermented at 28℃ for 3 days to obtain bacterial cellulose; take out the bacterial cellulose, and leave 20% fermentation broth in the fermentation device, ...
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[0029] Example 3
[0030] Collect the yellow syrup water produced during the production of tofu, add 1% fructose, 1% sucrose, 1% maltose and 1% glucose and pasteurize it; the ratio of the number of freshly activated bacteria is 10: 2:3:5 Gluconacetobacter xylinus (Gluconacetobacter xylinus, purchased from China General Microbial Culture Collection and Management Center, and the deposit number is CGMCC 1.1812), Gluconacetobacter sp., purchased from China Industrial Microorganism The strain preservation management center, the strain preservation number is CICC 10773), Saccharomyces cerevisiae (Saccharomyces cerevisiae, the strain was purchased from the China General Microbial Culture Collection and Management Center, the strain preservation number is CGMCC 2.3888), Hansen Debaryomyces ( Debaryomyces hansenii, the strain was purchased from the China Common Microbial Culture Collection and Management Center, the deposit number is CGMCC 2.3948), the total number of colonies reached 10...
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