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Electrochemical immunosensor for AFB1 (Aflatoxin B1), preparation method thereof and application thereof to detection of AFB1

An immunosensor, aflatoxin technology, applied in the direction of material electrochemical variables, graphene, nano-carbon, etc., can solve the problems of cumbersome detection process, long time, poor sensitivity, etc., and achieve the effect of good sensing performance

Active Publication Date: 2018-07-24
HONGHE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have the following disadvantages: the detection process is cumbersome, time-consuming, and labor-intensive; the equipment is expensive and the operation is complicated, and it is difficult to achieve rapid analysis; the sensitivity is poor

Method used

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  • Electrochemical immunosensor for AFB1 (Aflatoxin B1), preparation method thereof and application thereof to detection of AFB1
  • Electrochemical immunosensor for AFB1 (Aflatoxin B1), preparation method thereof and application thereof to detection of AFB1
  • Electrochemical immunosensor for AFB1 (Aflatoxin B1), preparation method thereof and application thereof to detection of AFB1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Preparation of Au nanorods and Au@Pt nanorods

[0052] Instruments used in Table 1

[0053]

[0054] Table 2 - Reagents used

[0055]

[0056] 1. Preparation of Au nanorods

[0057] 1) Preparation of gold nano-seed crystals: at a constant temperature of 25°C, first mix 5.0 mL, 0.20 M cetyltrimethylammonium bromide (CTAB) solution with 5.0 mL, 0.0005 M auric acid chloride (HAuCl 4 ) solution was mixed evenly to form mixed solution A; 4 ) solution to form a light brown mixed solution B; stir rapidly for 2 minutes, and keep the reaction system of the mixed solution B in the dark for 2 hours.

[0058] 2) Preparation of growth solution: at a constant temperature of 25°C, mix 5.0mL, 0.20M CTAB solution with 5.0mL, 0.0010M HAuCl 4 Mix the solution evenly to form a brownish yellow mixed solution C; add 0.2mL, 0.0040M silver nitrate (AgNO 3 ) solution, mixed evenly to form a mixed solution D.

[0059] 3) Add 70 μL of 0.0788M ascorbic acid (V c ) solution,...

Embodiment 2

[0069] Embodiment 2: the preparation of boron-sulfur co-doped graphene (BS-G)

[0070] Table 3 - Main Instruments

[0071]

[0072]

[0073] Table 4 - Main Reagents

[0074]

[0075] 1. Preparation of BS-G

[0076] Accurately weigh 10 mg of graphene oxide (GO), put it into a beaker containing 20 mL of ultrapure water and sonicate for 2 h to make GO uniformly dispersed, press GO, boron oxide (B 2 o 3 ) and sodium sulfide (Na 2 The mass ratio of S) is the ratio of 1:25:5, add 250mg B 2 o 3, sonicate for 30min, then add 50mg Na 2 S ultrasonication 5min, make B 2 o 3 and Na 2 S was uniformly dispersed in the GO solution, and finally the mixture was put into a microwave digestion apparatus, and reacted at 200°C for 3 hours to obtain BS-G.

[0077] 2. Characterization of BS-G

[0078] Use scanning electron microscope (SEM, JIB-4700F) to carry out characterization, the result is as follows Figure 8 As shown, BS-G presents a disordered, curled, wrinkled, and poro...

Embodiment 3

[0081] Example 3 BS-G / Au@Pt nanorod composite material constructs aflatoxin B 1 Immunosensor Table 5 - Main Instruments

[0082]

[0083] Table 6 - Main Reagents

[0084]

[0085] 1. Preparation of BS-G / Au@Pt nanorod composites:

[0086] Take 1 mL of BS-G prepared in Example 2 above and sonicate it in a centrifuge tube for 30 min, add 7 mL of Au@Pt nanorods, and shake on a vortex mixer for 40 min to obtain BS-G / Au@Pt nanorod composites .

[0087] 2. Preparation of Electrochemical Immunosensors

[0088] a) Modified gold electrode

[0089] Al 2 o 3 After polishing on the solution, successively use H 2 o 2 and H 2 SO 4 The solution (1:3) was sonicated for 3min, cleaned with ultrapure water for 1min, dried in the air, and contained 0.5M, KCl and 1.0mM, K 3 Fe(CN) 6 The electrode stability was tested in the solution. Take 7 μL of the prepared BS-G / Au@Pt nanorod composite material and drop-coat it on the surface of the gold electrode, and let it dry naturally at r...

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Abstract

The invention discloses a preparation method of an electrochemical immunosensor for detecting aflatoxin B1. The preparation method comprises the following steps of 1), preparing a gold nanorod; 2), cladding the gold nanorod with a platinum shell, so as to form a gold and platinum core-shell type nanorod; 3), synthesizing boron and sulfur codoped graphene by adopting a microwave-assisted hydrothermal method; 4), embedding the gold and platinum core-shell type nanorod into the lamellas of the boron and sulfur codoped graphene by utilizing an ultrasonic technique, so as to obtain a boron and sulfur codoped graphene loaded gold and platinum core-shell type nanorod composite material; 5), dropwise coating boron and sulfur codoped graphene loaded gold and platinum core-shell type nanorod composite material in the step 4) on the surface of a gold electrode to modify the gold electrode, and dropwise coating an aflatoxin B1 antibody, bovine albumin and an aflatoxin B1 antigen on the surface ofthe well modified gold electrode in sequence, so as to obtain the electrochemical immunosensor for detecting the aflatoxin B1. The electrochemical immunosensor has the beneficial effects that the electrochemical immunosensor is quick in response speed and good in reproducibility, can be used for quickly determining an actual sample, and has a certain advantage compared with an existing detection method.

Description

technical field [0001] The invention relates to the technical field of nanomaterial preparation, in particular to a method for preparing boron-sulfur co-doped graphene-loaded gold-platinum core-shell nanorods, and using boron-sulfur co-doped graphene-loaded gold-platinum core-shell nanorods for Build AFBs 1 Electrochemical immunosensor, and for aflatoxin B 1 detection. Background technique [0002] Aflatoxin is widely found in crops and food. It is a metabolite produced by Aspergillus flavus and Aspergillus parasiticus. It is a highly toxic and highly toxic substance. It was discovered in the 1960s and is a group of compounds with similar chemical structures. , whose structure is characterized by containing a difuran ring and an oxa-naphthalenone, more than 20 species have been found so far, and aflatoxin B is the one whose structure is confirmed 1 , B 2 , G 1 , M 1 , M 2 And so on 18 kinds. Aflatoxin has strong carcinogenicity. In 1993, it was classified as a Class ...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26G01N27/30C01B32/184
Inventor 陈显兰刘卫张国伟杨光明冯绍平苟高章吴娜
Owner HONGHE COLLEGE
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