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Ultra-infiltration nano branched gold SERS micro-chip and preparation method thereof

A super-wetting, microchip technology, applied in the field of material preparation and SERS detection and analysis, can solve problems such as droplet diffusion, and achieve the effects of simple preparation, broad application prospects and less sample demand.

Inactive Publication Date: 2018-08-07
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above technical problems, the present invention provides a super-wetting nano-dendritic gold SERS microchip and a preparation method thereof, wherein the hydrophilic points distributed on the super-hydrophobic surface of the super-wetting nano-dendritic gold SERS microchip can fix droplets and Enrichment of the substance to be tested solves the problem of droplet diffusion in SERS trace detection

Method used

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  • Ultra-infiltration nano branched gold SERS micro-chip and preparation method thereof
  • Ultra-infiltration nano branched gold SERS micro-chip and preparation method thereof
  • Ultra-infiltration nano branched gold SERS micro-chip and preparation method thereof

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Embodiment 1

[0040] This embodiment provides a super-wetting nano-dendritic gold SERS microchip, the super-wetting nano-dendritic gold SERS microchip sequentially includes: ITO glass, titanium layer, gold layer, nano-dendritic gold layer, superhydrophilic array- Super-hydrophobic surface; the super-hydrophilic array-super-hydrophobic surface forms a super-hydrophilic array point by destroying part of the hydrophobic structure on the super-hydrophobic surface; the super-hydrophilic array point has a nano-dendritic gold layer structure, And the super-hydrophilic array point is used for immobilizing the droplet to be detected and concentrating and enriching the detected substance, and the nano-dendritic gold layer structure of the super-hydrophilic array point can enhance the Raman signal.

[0041] The superhydrophilic array dots include at least two or more hydrophilic dots;

[0042] By adding different concentrations of liquid droplets to be detected onto different hydrophilic points of the...

Embodiment 2

[0045] This embodiment provides a method for preparing a nano-dendritic gold super-wetting SERS microchip, which is used to prepare a nano-dendritic gold super-wetting SERS microchip described in Example 1,

[0046] Specifically:

[0047] Preparation of nano-dendritic gold substrates required for SERS detection ( figure 1 ): Cut the whole piece of ITO glass into small pieces of 3×1.5cm with an ITO conductive glass knife, and in 100°C piranha washing solution (98%H 2 SO 4 : 30%H 2 o 2 , V / V=3:1) soaking and cleaning for 1h, taking out the glass piece and immersing it in acetone solution for ultrasonic cleaning for 30min, then placing it in ethanol and ultrapure water for ultrasonic cleaning for 30min respectively, taking out the glass piece and drying it with nitrogen gas for later use. Measure the surface resistance of the glass with the ohm gear of a multimeter. The side with resistance is the conductive side of ITO glass. The conductive side is cleaned in an oxygen plasm...

Embodiment 3

[0051] SERS detection of rhodamine 6G on hydrophilic array spot:

[0052] The super-wetting nano-dendritic gold SERS microchip with hydrophilic arrays prepared in Example 2 was cleaned with ultrapure water to ensure that the hydrophilic sites were clean. Add 2 μL of 10 drops to each of the eight hydrophilic sites -3 M,10 -4 M,10 -5 M,10 -6 M,10 -7 M,10 -8 M,10 -10 M, and 10 -12 M’s rhodamine 6G aqueous solution, wait for 15 minutes at room temperature to evaporate the water completely, use a micro confocal laser Raman spectrometer to perform SERS detection on different points at a wavelength of 532nm, change the detection position by moving the microchip, and complete within 5 minutes To the detection of eight concentrations of rhodamine 6G, obtain the Raman spectrum with rhodamine 6G characteristic peak ( Figure 5 ), the integrated data map obtained with increasing Raman intensity as the concentration increases.

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Abstract

The invention mainly belongs to the technical field of material preparation and SERS detection and analysis, and particularly relates to an ultra-infiltration nano branched gold SERS micro-chip and apreparation method thereof. The ultra-infiltration nano branched gold SERS micro-chip can be used for detecting biomolecule miRNA. The ultra-infiltration nano branched gold SERS micro-chip successively comprises ITO glass, a titanium layer, a gold layer, a nano branched gold layer and a super-hydrophilic array-super-hydrophobic surface; and the super-hydrophilic array-super-hydrophobic surface isspecifically prepared by arranging super-hydrophilic array points on a super-hydrophobic surface in a distributed manner, and the super-hydrophilic array points are used for fixing to-be-detected liquid drops. To-be-detected liquid drops with different concentrations are dropwise added on different hydrophilic points of the same SERS micro-chip, and the to-be-detected liquid drops with the different concentrations can be simultaneously detected to improve detecting accuracy and shorten detecting time.

Description

technical field [0001] The invention mainly belongs to the technical field of material preparation and SERS detection and analysis, and specifically relates to a super-wetting nano-dendritic gold SERS microchip and a preparation method thereof, and the super-wetting nano-dendritic gold SERS microchip can be used to detect biomolecules miRNA detection. Background technique [0002] Surface Raman Enhancement (SERS) is due to the rough substrate noble metal material (gold, silver and copper) being illuminated, the plasmon on the surface of the material is excited to a high energy level and coupled with the electric field to resonate, so that the electric field on the metal surface is enhanced, resulting in enhanced Raman scattering. SERS technology is widely used due to its advantages of no need for sample pretreatment, simple operation, and fast detection speed, such as the detection of nitrate heavy metal ions in water quality, the quantitative detection of melamine and rhod...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y30/00
CPCB82Y30/00G01N21/658
Inventor 许太林宋永超许利苹张学记王树涛
Owner UNIV OF SCI & TECH BEIJING
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