Method for preparing organic flower fertilizer
A flower fertilizer, organic technology, applied in the field of organic flower fertilizer preparation, can solve the problems of low fertilizer efficiency and slow flower absorption, and achieve the effect of enhancing fertilizer efficiency, promoting growth and nutrient absorption, and improving accessibility
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[0020] A preparation method of organic flower fertilizer, comprising the steps of:
[0021] (1) Take Phanerochaete chrysosporium and inoculate it into PDA medium according to the inoculum amount of 2%, and cultivate it at 28~30℃ for 2~3 days to obtain a culture. Take the culture and add sterile water at a mass ratio of 1:10 , shake culture at 25~30°C for 2~3h to obtain spore suspension, take vinegar grains and add enzyme production medium at a mass ratio of 1:25, sterilize at 121°C for 20min, cool to room temperature, obtain cooling product, and take spore suspension The inoculum was inoculated into the cooling material according to the inoculum amount of 30%, and cultured at 25-30°C for 3-5 days to obtain culture A;
[0022] (2) Take the culture A and mix it with sterile water at a mass ratio of 1:22, and then ultrasonically treat it at 420W and 35~40°C for 30~40min to obtain the sonicated product. Mix in 4.8 acetic acid-sodium acetate buffer to obtain a mixture, take the mi...
Embodiment 1
[0026] Vinegar grains: taken from a vinegar production factory, mainly containing 11.40% crude protein, 12.21% crude fat, and 35.87% crude fiber.
[0027] Mushroom dregs: purchased from Zhongshan Pasteur Agricultural Technology Co., Ltd., pH 6.05, mainly containing 25.6% organic matter, 1.55% total phosphorus, 1.53% total nitrogen, and 1.20% total potassium.
[0028] Trace element mixed solution: in parts by mass, take 1 part of glycine, 3 parts of MgSO 4 ·7H 2 O, 0.5 parts of MnSO 4 ·7H 2 O, 1 part NaCl, 0.1 part FeSO 4 ·7H 2 O, 0.2 part of CoCl·6H 2 O, 0.1 part of ZnSO 4 ·7H 2 O, 0.2 parts of CuSO 4 ·5H 2 O, 0.001 part of KAl(SO 4 ) 2 12H 2 O, 0.02 parts of H 3 BO 4 , 0.01 parts of Na 2 MoO 4 2H 2 O mixed, pH 6, that is.
[0029] Enzyme production medium: by mass parts, take 10 parts of glucose, 2 parts of ammonium tartrate, and 1 part of VB 1 , 0.5 parts of veratrol, 90 parts of sodium acetate buffer solution with a concentration of 0.2mol / L, 20 parts of...
Embodiment 2
[0036] Vinegar grains: taken from a vinegar production factory, mainly containing 11.40% crude protein, 12.21% crude fat, and 35.87% crude fiber.
[0037] Mushroom dregs: purchased from Zhongshan Pasteur Agricultural Technology Co., Ltd., pH 6.05, mainly containing 25.6% organic matter, 1.55% total phosphorus, 1.53% total nitrogen, and 1.20% total potassium.
[0038] Trace element mixed solution: in parts by mass, take 3 parts of glycine, 5 parts of MgSO 4 ·7H 2 O, 0.8 parts of MnSO 4 ·7H 2 O, 3 parts of NaCl, 0.4 parts of FeSO 4 ·7H 2 O, 0.5 part of CoCl·6H 2 O, 0.3 parts of ZnSO 4 ·7H 2 O, 0.4 parts of CuSO 4 ·5H 2 O, 0.003 part of KAl(SO 4 ) 2 12H 2 O, 0.04 parts of H 3 BO 4 , 0.04 parts of Na 2 MoO 4 2H 2 O mixed, pH 6.5, that is.
[0039] Enzyme production medium: by mass parts, take 15 parts of glucose, 5 parts of ammonium tartrate, and 3 parts of VB 1 , 0.8 parts of veratrol, 100 parts of sodium acetate buffer solution with a concentration of 0.2mol / ...
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