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Method for extracting polysaccharides in Sibiraea angustata (Rehd.)Hand.-Mazz

A technology of willow tea and polysaccharides, which is applied in the field of extracting polysaccharides in willow tea, can solve the problems of taking several or even ten hours, easily destroying the structure of polysaccharides, and reducing bioavailability, so as to protect the liver, shorten the extraction time, and improve The effect of immunity

Inactive Publication Date: 2018-08-10
CHONGQING MEDICAL & PHARMA COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The polysaccharides in willow tea can be widely used in the development of medicine, biological health care and functional food, etc. However, the traditional extraction method takes several or even ten hours to extract, takes a long time, the extraction purity is low, and the acid and alkali are very high. It is easy to destroy the structure of polysaccharides and reduce the bioavailability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A method for extracting polysaccharides in willow tea, specifically comprising the steps of:

[0050] A. Raw material handling:

[0051] Take dried willow tea, remove impurities such as deteriorated or unqualified willow tea, grind the selected willow tea, and further pass the pulverized willow tea powder through a 40-mesh sieve;

[0052] B. Configuration of enzyme solution:

[0053] Cellulase, pectinase and papain were mixed with distilled water at a mass ratio of 1:40 to prepare an enzyme solution; the amount of enzyme added was 300U / g of cellulase, 5000U / g of pectinase, and 15000U / g of papain ;

[0054] C. Enzymatic hydrolysis:

[0055] Mix 1 g of willow tea powder obtained in step A with 10 mL of enzyme solution obtained in step B for enzymolysis;

[0056] D. Ultrasonic extraction:

[0057] The mixed solution obtained in step C is ultrasonicated for 25 minutes in an ultrasonic wave with an ultrasonic power of 500W, and the extract can be obtained after centrifu...

Embodiment 2

[0070] A. Raw material handling:

[0071] Take dried willow tea, remove impurities such as deteriorated or unqualified willow tea, grind the selected willow tea, and further pass the pulverized willow tea powder through a 50-mesh sieve;

[0072]B. Configuration of enzyme solution:

[0073] Cellulase, pectinase and papain were mixed with distilled water at a mass ratio of 1:45 to prepare an enzyme solution; the amount of enzyme added was 300U / g of cellulase, 5000U / g of pectinase, and 15000U / g of papain

[0074] C. Enzymatic hydrolysis:

[0075] Mix 1 g of willow tea powder obtained in step A with 30 mL of enzyme solution obtained in step B for enzymolysis;

[0076] D. Ultrasonic extraction:

[0077] The mixed solution obtained in step C is ultrasonicated for 30 minutes in an ultrasonic wave with an ultrasonic power of 500 W, and the extract can be obtained after centrifugal filtration;

[0078] E. Precipitation:

[0079] Concentrate the extract obtained in step D to 1 / 2 of...

Embodiment 3

[0090] A. Raw material handling:

[0091] Take dried willow tea, remove impurities such as deteriorated or unqualified willow tea, crush the selected willow tea, and further pass the crushed willow tea powder through a 60-mesh sieve;

[0092] B. Configuration of enzyme solution:

[0093] Cellulase, pectinase, and papain were mixed with distilled water at a mass ratio of 1:50 to prepare an enzyme solution; the amount of enzyme added was 300 U / g of cellulase, 5,000 U / g of pectinase, and 15,000 U / g of papain

[0094] C. Enzymatic hydrolysis:

[0095] Mix 1 g of willow tea powder obtained in step A with 50 mL of enzyme solution obtained in step B, and perform enzymatic hydrolysis;

[0096] D. Ultrasonic extraction:

[0097] The mixed solution obtained in step C is ultrasonicated in an ultrasonic wave with an ultrasonic power of 500W for 45 minutes, and the extract can be obtained after centrifugal filtration;

[0098] E. Precipitation:

[0099] Concentrate the extract obtaine...

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PUM

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Abstract

The invention belongs to the field of drug extraction, and particularly relates to a method for extracting polysaccharides in Sibiraea angustata (Rehd.)Hand.-Mazz. The method comprises the following steps that 1, dried Sibiraea angustata (Rehd.)Hand.-Mazz is obtained, selected and smashed; 2, an enzyme solution is prepared with distilled water; 3, the Sibiraea angustata (Rehd.)Hand.-Mazz power ismixed with the enzyme solution for step-by-step enzymolysis; 4, the mixed solution is extracted in ultrasonic waves, and an extracting solution can be obtained after centrifugal filtration; 5, the extracting solution is subjected to vacuum concentration, then absolute ethyl alcohol is added, and the mixture of the extracting solution and the absolute ethyl alcohol is fully stirred and evenly mixed; 6, the mixed solution is placed in a refrigerator for standing, a precipitate is obtained; 7, the precipitate is dissolved with distilled water, and pure Sibiraea angustata (Rehd.)Hand.-Mazz polysaccharides can be obtained after freeze drying. The method is short in extracting time and high in extracting purity. By using the method, the degree of damage to structures of the polysaccharides is low, and the polysaccharides can effectively work in the aspects of improving immunities, resisting tumors, resisting viruses, resisting aging, resisting infection, resisting ulcer, decreasing blood sugar, resisting radiation and protecting the liver.

Description

technical field [0001] The invention belongs to the field of medicine extraction, and in particular relates to a method for extracting polysaccharides in willow tea. Background technique [0002] Willow tea is a kind of Sibiraea angustata (Rehd.) Hand.-Mazz., a seed plant of the genus Xianbei in the Rosaceae family. It is mainly distributed in southeastern Gansu, southern Qinghai, Sichuan, the back of Yunnan and Tibet, etc. land. Willow tea is one of the commonly used Tibetan folk medicines. It is rich in a variety of triterpenoids, organic acids, esters and alkanes, such as: oleanolic acid, ursolic acid, vanillic acid, caffeic acid, Xianbei ester, Various active ingredients such as lupine acid, lupeol, rutin, catechin and willow tea polysaccharides (drinking soup, glucose, galactose, etc.). Willow tea polysaccharides have the functions of digesting stagnation, evacuating wind-heat, regulating lipid metabolism, and protecting the liver. [0003] Polysaccharides are active...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A61P35/00A61P31/12A61P39/06A61P31/00A61P3/10A61P39/00A61P1/16
CPCA61P1/16A61P3/10A61P31/00A61P31/12A61P35/00A61P37/04A61P39/00A61P39/06C08B37/0003
Inventor 朱仝飞闫志慧
Owner CHONGQING MEDICAL & PHARMA COLLEGE