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Anti-radiation skin care composition

A technology of skin care composition and lysate, which is applied in the field of beauty and makeup, and can solve the problems of increased irritation, photosensitivity, roughness, and sensitivity symptoms, etc., and achieve the effect of enhancing skin barrier function, improving skin sensitivity, and repairing sensitive skin

Inactive Publication Date: 2018-08-17
ZHEJIANG ORIGIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no good way to make skin, especially sensitive skin, resist ultraviolet rays at present. Commonly used sunscreens and other skin care products contain sunscreen agents, alcohol and other ingredients that increase unsafe factors such as irritation and photosensitivity, and increase the risk of aging. Chance of developing allergies
Moreover, in the actual investigation, it was found that even if there is no obvious irritation and allergic reaction, long-term application of highly irritating and allergen-containing cosmetics will damage the skin and cause symptoms such as roughness, bumps, and itching

Method used

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  • Anti-radiation skin care composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A composition for skin care, comprising the following preparation steps:

[0025] 1. Preparation of Neural Stem Cell Lysates

[0026] The human neural stem cells were cooled in liquid nitrogen for 30 minutes, thawed in a cell culture incubator at 37°C, and repeated 3 times; the transmembrane was collected by filtration with a 250nm pore size membrane, and the product was obtained. Put it in 37 ℃ and keep warm for later use.

[0027] 2. Preparation of skin stem cell culture supernatant:

[0028] (1) Prepare medium: basal medium is DMEM / F12, 25μg / L VEGF, 35μg / L EGF, 8μg / L NGF, 20mg / L human serum albumin, 25μg / L vitamin E, ferric citrate 2mmol / L, glutamine 2mmol / L, gentamicin 50g / L, putrescine 1.6mg / L.

[0029] (2) Culture human skin stem cells in the medium of step (1), culture at 37°C for 12 hours, and then use 5mJ / cm 2 The cells were continuously irradiated with ultraviolet light for 18 hours, and cultured for 6 hours after the irradiation.

[0030] (3) Add 1 wt% ...

Embodiment 2

[0036] A composition for skin care, comprising the following preparation steps:

[0037] 1. Preparation of Neural Stem Cell Lysates

[0038] The human neural stem cells were cooled in liquid nitrogen for 30 minutes, thawed in a cell culture incubator at 37°C, and repeated 4 times; the transmembrane was collected by filtration with a 300nm pore size membrane, and the product was obtained. Put it in 37 ℃ and keep warm for later use.

[0039] 2. Preparation of skin stem cell culture supernatant:

[0040] (1) Prepare medium: basal medium is DMEM / F12, 28μg / L VEGF, 28μg / L EGF, 8μg / L NGF, 20mg / L human serum albumin, 25μg / L vitamin E, 2.5mmol ferric citrate / L, glutamine 1.6mmol / L, gentamicin 50g / L, putrescine 1.5mg / L.

[0041] (2) Inoculate human skin stem cells in the medium of step (1), culture at 37°C for 24 hours, and then use 7mJ / cm 2 The cells were continuously irradiated with ultraviolet rays for 5 hours, and cultured for 12 hours after the irradiation.

[0042] (3) Add 2...

Embodiment 3

[0047] A composition for skin care, comprising the following preparation steps:

[0048] 1. Preparation of Neural Stem Cell Lysates

[0049]The human neural stem cells were cooled in liquid nitrogen for 30 minutes, thawed in a cell culture incubator at 37°C, and repeated 3 times; the transmembrane was collected by filtration with a 450nm pore size membrane to obtain the final product. Put it in 37 ℃ and keep warm for later use.

[0050] 2. Preparation of skin stem cell culture supernatant:

[0051] (1) Prepare medium: basal medium is DMEM / F12, 25μg / LVEGF, 40μg / L EGF, 5μg / L NGF, 35mg / L human serum albumin, 20μg / L vitamin E, 1mmol / L ferric citrate , glutamine 1.2mmol / L, gentamicin 80g / L, putrescine 1.0mg / L.

[0052] (2) Culture human skin stem cells in the medium of step (1), culture at 37°C for 6 hours, and then use 3mJ / cm 2 Cells were continuously irradiated with ultraviolet light for 22 hours, and cultured for 3 hours after the irradiation.

[0053] (3) Add 0.8wt% of the...

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PUM

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Abstract

The invention provides an anti-radiation skin care composition which is prepared from skin stem cell culture supernatant fluid, wherein the skin stem cells are irradiated by low-dosage ultraviolet light. The anti-radiation skin care composition disclosed by the invention improves a defense function of the skin to radiation damage. Even if the anti-radiation skin care composition is applied to sensitive skin which is extremely sensitive to external stimulation, a very good anti-radiation effect can be also achieved. According to the anti-radiation skin care composition disclosed by the invention, all the effective ingredients are in synergistic interaction, so that a skin barrier function is enhanced, immunity of damaged skin is improved, and varieties of symptoms of cutaneous sensibility and the like are improved.

Description

technical field [0001] The invention belongs to the field of beauty and makeup, and mainly relates to a skin care composition. Background technique [0002] The skin is the main defense barrier of the human body. The skin barrier is the natural guard of the human skin. Everyone has a natural skin barrier on the surface of the skin. Its function is to protect various organs in the human body from changes in temperature, humidity, etc., and It resists the stimuli of the external environment on the human body, such as ultraviolet rays, pollutants, etc., and plays an important role in maintaining homeostasis. However, ultraviolet rays, excessive physical and chemical stimuli from the external environment, stress, and nutritional deficiencies degrade the normal function of the skin, damage the skin barrier, cause keratinization, and form wrinkles. Among them, radiation from the outside, such as ultraviolet rays, is an unavoidable stimulus for the skin, and is an important factor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/35A61K8/44A61K8/49A61K8/60A61K8/65A61K8/9794A61Q17/04
CPCA61K8/99A61K8/355A61K8/442A61K8/4946A61K8/606A61K8/65A61K8/9794A61K2800/805A61Q17/04
Inventor 江克光谭德松何祖法
Owner ZHEJIANG ORIGIN BIOTECH
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