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A kind of gradient composite material and its preparation method and application

A composite material and gradient technology, applied in medical science, prosthesis, tissue regeneration, etc., to achieve the effect of controllable process, easy operation, and good hydrophilicity

Active Publication Date: 2020-10-13
上海积力威尔生物科技合伙企业(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of gradient composite material with high-efficiency promotion of osteogenesis and fibrogenesis and can significantly improve the strength of tendon-bone bonding and its preparation method and application, so as to solve the problems existing in existing tendon-bone healing materials. one or more technical issues

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  • A kind of gradient composite material and its preparation method and application
  • A kind of gradient composite material and its preparation method and application
  • A kind of gradient composite material and its preparation method and application

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Experimental program
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preparation example Construction

[0032] The present invention provides a kind of preparation method of gradient composite material in the first aspect, described method comprises the following steps:

[0033] (1) using an organic solvent to prepare biocompatible polymers and bioactive ceramic powders into various slurries with different contents of bioactive ceramic powders; and

[0034] (2) The various slurries prepared in step (1) are cast layer by layer or co-pressed by layered casting in a manner of increasing or decreasing the content of bioactive ceramic powder, to obtain a gradient composite comprising a multi-layer cast film Material.

[0035] In the present invention, the multiple refers to two or more, and the multi-layer refers to two or more layers; the layer-by-layer casting refers to placing a certain volume of a slurry Cast in the casting machine to obtain the first layer of cast film, and then place another slurry in the casting machine on the basis of the first layer of cast film to obtain t...

Embodiment 1

[0057] ① Mix polycaprolactone and silicon calcium phosphate powder in four 50mL sealed bottles according to different volume ratios of 1:0, 7:3, 5:5 and 3:7, and then pour into each sealed bottle Add a certain amount of dichloromethane to dissolve the polycaprolactone, and after the polycaprolactone is completely dissolved, four kinds of slurries with different volume percentages of uniformly dispersed silicon calcium phosphate are obtained.

[0058] ② The 4 kinds of slurries obtained in ① were casted in layers using the preparation parameters in Table 1 to prepare 4 kinds of single-layer cast films with increasing volume percentages of calcium phosphate calcium phosphate. In Table 1, the casting volume represents the amount of each of the slurries used for casting.

[0059] 4 kinds of single-layer casting films that present embodiment make carry out performance test:

[0060] figure 1 The backscattered surface topography (SEM image) of the four single-layer cast films prepa...

Embodiment 2

[0062] Carry out mouse osteoblast and fibroblast adhesion experiment respectively on 4 kinds of single-layer casting films that embodiment 1 obtains: After every kind of single-layer casting film samples are sterilized by 75% alcohol for 2 hours, the density is 5×104 1 mL of cell suspension per mL was inoculated on the surface of the sample, and after culturing for a specific time (1, 4, 24 h), each single-layer cast film sample was washed twice with phosphate buffered saline (PBS buffer), and the mass fraction Fix cells with 4% paraformaldehyde, wash with PBS buffer, add 500 μL volume of 0.1% Triton 100 for cell permeabilization, and finally use rhodamine dye and 4,6-diamidine- The cytoskeleton and nucleus were stained with 2-phenylindole (DAPI), and the cytoskeleton and nucleus were observed under a fluorescent microscope.

[0063] figure 2 It is the experimental result of observing the morphology of osteoblasts on the surface of four kinds of single-layer cast film sample...

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Abstract

The invention relates to a gradient composite material and a preparation method and an application thereof. The method comprises the steps: preparing a variety of slurries with different contents of bioactive ceramic powder from a biocompatible polymer and the bioactive ceramic powder with an organic solvent; and carrying out layer-by-layer tap casting or layering tap casting co-pressing of a variety of slurries in a manner of progressively increasing or decreasing the contents of the bioactive ceramic powder, to prepare the gradient composite material containing a plurality of layers of tap-casted films. The gradient composite material preferably contains four layers of tape-casted films, and the volume percentage contents of the bioactive ceramic powder are 0%, 30%, 50% and 70% from thefirst-layer tape-casted film to the fourth-layer tape-casted film respectively. The prepared gradient composite material can promote the early adhesion and proliferation of osteoblasts and fibroblastsand effectively promote the formation of transition layers between tendons and bone tissues, and the tendon-bone bonding strength can be increased by 38.6%. The gradient composite material provided by the invention can be used as a tendon-bone healing patch material, and is a quite potential biomedical material.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a gradient composite material for tendon-bone healing, a preparation method and application thereof. Background technique [0002] The tendon-bone insertion is an important area connecting two different tissues, tendon-bone, and plays a key role as a bridge between tendon and bone. A survey study found that among people over the age of 50, the probability of partial or complete tearing of the tendon-bone insertion of the rotator cuff is as high as 30-50%. In China, with the popularization of national fitness and people's increasing emphasis on health, the probability of tendon-bone injury caused by excessive or improper exercise is also increasing year by year. [0003] The tendon-bone insertion connects a millimeter-sized and highly ordered layered site of disparate tendon and bone phases. In the physiological mechanics test, it is found...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/10A61L27/12A61L27/18A61L27/50
CPCA61L27/10A61L27/12A61L27/18A61L27/50A61L2430/02A61L2430/10C08L67/04
Inventor 宁聪琴郭劲书赵金忠苏为
Owner 上海积力威尔生物科技合伙企业(有限合伙)