Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of anidulafungin precursor

A polymer, echinocandin technology, applied in the direction of peptide, fermentation, etc., can solve the problems of negative effect of deacylase activity, cumbersome extraction process of crude enzyme, unfavorable scale-up production, etc., achieve low cost, improve conversion speed and enzyme stable and easy to operate

Pending Publication Date: 2018-08-17
BRIGHTGENE BIO MEDICAL TECH (SUZHOU) CO LTD +1
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the crude enzyme extraction process is cumbersome, which is not conducive to industrial scale-up production; the extraction of crude enzyme by rotary steaming is easy to cause protein denaturation due to high temperature, and the activity of deacylase has a negative impact
[0012] The enzymes used in the currently reported cross-linked enzyme polymers include hydrolases, lyases, and oxidoreductases. At present, there are no reports on the fermentation of semi-synthetic drugs, and there are no reports on the application of this technology to deacylases.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 produces the fermentation of deacylase bacterial strain and crude enzyme preparation

[0031] Bacterial species: Streptomyces albus (Streptomyces albus), preservation number: ATCC21725; -80°C cryopreservation tube;

[0032] Seed medium: 0.5% hot-fried soybean cake powder (w / v, the same below), 0.5% yeast powder, 0.5% peptone, 1% glucose, pH about 6.8-7.2, cultured at 30°C for 1-2 days;

[0033] Fermentation medium: hot fried soybean cake powder 1%, yeast powder 1%, peptone 1%, glucose 3%, sodium chloride 0.5%, magnesium sulfate heptahydrate 0.2%, dipotassium hydrogen phosphate 0.1%, pH about 6.8-7.2 , cultured at 30°C for 2 to 3 days;

[0034] Inoculate the genetically engineered strain of Streptomyces albicans on the seed medium, culture at 30°C for 1-2 days, then inoculate 5% of the fermentation volume into the fermentation medium, and cultivate at 30°C for 2-3 days;

[0035] After the fermentation is completed, filter the fermented liquid to obtain the s...

Embodiment 2

[0036] Example 2 Deacylase cross-linking conversion to produce echinocandin B nucleus

[0037] Prepare 1000 L of phosphate buffer solution in the reaction tank, containing 2.24 g / L of potassium dihydrogen phosphate and 1.24 g / L of disodium hydrogen phosphate, and adjust the pH to 6.0 with hydrochloric acid or sodium hydroxide.

[0038] Add 20 kg of crude enzyme and 2 kg of bovine serum albumin (BSA) to the phosphate buffer, feed air at a flow rate of 200 L per minute, stir for 30 minutes at a stirring speed of 80 rpm, and stand at 8° C. for 1 hour.

[0039] Add 20 mmol / L of glutaraldehyde to the reaction solution, stir at 30° C. for 2 hours, and the stirring speed is 100 rpm.

[0040] Add 10% echinocandin B dimethyl sulfoxide solution containing 20kg echinocandin B to the reaction solution, stir and react at 30°C for 12 to 48 hours, the stirring speed is 150rpm, and the echinocandin B in the conversion system is monitored by HPLC during the conversion process. The concentrati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel method of converting echinocandin B into echinocandin B parent nucleus. A crosslinked enzyme aggregation technique is applied to the process of conversion of echinocandin B into echinocandin B parent nucleus. The method comprises: preparing deacylase crosslinked enzyme aggregate with deacylase; allowing the crosslinked enzyme aggregate to convert echinocandin B intoechinocandin B parent nucleus. The preparation method enables the molar conversion rate to reach 85% to 90%; the production process is simplified; the cost is lowered.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to the preparation of an antifungal fermented semi-synthetic drug precursor, and more specifically to the preparation of anifungin precursor echinocandin B core. Background technique [0002] In recent years, due to the increasing number of immunocompromised patients, the incidence of fungal infections has increased significantly, especially the incidence and mortality of deep fungal infections. Echinocandin antibiotics are a group of natural products discovered in the 1970s, with similar cyclic polypeptide cores and different fatty acid side chains, which can non-competitively inhibit fungal cell wall β-1,3-glucan synthesis Enzyme activity, so as to achieve the purpose of antifungal. Compared with traditional antifungal drugs, this type of drug has a unique mechanism of action, low toxicity and side effects, and has strong antibacterial activity against some azole and amphote...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04
CPCC07K7/56
Inventor 袁建栋别一
Owner BRIGHTGENE BIO MEDICAL TECH (SUZHOU) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com