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Application of danshinolic acid components in preparing pharmaceutical preparations for improving dialysis rate and filtration rate of protein-bound uremic toxins

A technology for salvianolic acids and pharmaceutical preparations, which can be applied to medical preparations containing active ingredients, drug combinations, organic active ingredients, etc., and can solve the problem of low dialysis efficiency and filtration efficiency, and inability to remove protein-bound small-molecule urea toxins. , less proportion and other problems, to achieve the effect of improving dialysis efficiency and filtration efficiency

Active Publication Date: 2018-09-07
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used clinical dialysis (including hemodialysis and peritoneal dialysis) and hemofiltration and other extracorporeal renal replacement therapy strategies can effectively remove small-molecule uremic toxins, as well as medium-molecule uremic toxins, but cannot remove protein-bound small-molecule uremic toxins.
Because whether it is the artificially synthesized semipermeable membrane used in hemodialysis and hemofiltration or the patient's own peritoneum used in peritoneal dialysis, it can only allow the free form of uremic toxin in the blood circulation to pass through, while the protein-bound uremic toxin is due to the free form. There is a small proportion, and the dialysis efficiency and filtration efficiency are very low

Method used

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  • Application of danshinolic acid components in preparing pharmaceutical preparations for improving dialysis rate and filtration rate of protein-bound uremic toxins
  • Application of danshinolic acid components in preparing pharmaceutical preparations for improving dialysis rate and filtration rate of protein-bound uremic toxins
  • Application of danshinolic acid components in preparing pharmaceutical preparations for improving dialysis rate and filtration rate of protein-bound uremic toxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Danhong Injection Significantly Improves the Dialysis Efficiency of Protein-Bound Uretoxin

[0020] 1. Experimental method

[0021] 1.1 Sample preparation method

[0022] Preparation of standard samples: Accurately weigh indoxyl sulfate (IS) and p-cresol sulfate (PCS) standards, dissolve them in 200 μl ultrapure water, and prepare a standard solution with IS and PCS concentrations of 50 μg / ml.

[0023] Preparation of control samples: Take 165 μl of rat plasma sample, add 10 μl of IS aqueous solution and 5 μl of PCS aqueous solution, vortex for 30 s to mix thoroughly, then let stand at room temperature for 30 min, add 20 μl of ultrapure water, vortex for 30 s to fully mix. The total volume of dialysate was 200 μl, and the concentrations of IS and PCS were both 50 μg / ml.

[0024] Danhong injection sample preparation: Take 165 μl of rat plasma sample, add 10 μl of IS aqueous solution and 5 μl of PCS aqueous solution, vortex for 30 s to mix thoroughly, and then ...

Embodiment 2

[0033] Example 2 Salvianolic acids significantly improve the dialysis efficiency of protein-bound uremic toxins

[0034] 1. Experimental method

[0035] 1.1 Sample preparation method

[0036] Preparation of control samples: Take 180 μl of rat plasma sample, add 10 μl of IS aqueous solution and 5 μl of PCS aqueous solution, vortex for 30 s to mix thoroughly, then let stand at room temperature for 30 min, and add 5 μl of ultrapure water. The total volume of dialysate was 200 μl, and the concentrations of IS and PCS were both 50 μg / ml.

[0037] Salvianolic acid sample preparation: Take 180 μl of rat plasma sample, add 10 μl of IS aqueous solution and 5 μl of PCS aqueous solution, vortex for 30 seconds to mix thoroughly, and then let it stand at room temperature for 30 minutes, add high concentration (50 μg / ml), medium concentration (10 μg / ml) respectively ml), low concentration (5μg / ml) of shikonian acid, salvianolic acid A, salvianolic acid, caffeic acid, salvianolic acid B, s...

Embodiment 3

[0041] Example 3 Danhong Injection Significantly Improves the Filtration Efficiency of Protein-Bound Uretoxin

[0042] 1. Experimental method

[0043] 1.1 Sample preparation method

[0044] Preparation of standard samples: Accurately weigh IS and PCS standards, dissolve them in 200 μl ultrapure water, and prepare a standard solution with IS and PCS concentrations of 50 μg / ml.

[0045] Preparation of control samples: Take 165 μl of human mixed plasma sample, add 10 μl of IS aqueous solution and 5 μl of PCS aqueous solution, vortex for 30 s to mix thoroughly, then let stand at room temperature for 30 min, add 20 μl of ultrapure water, vortex for 30 s to fully mix. The total volume of dialysate was 200 μl, and the concentrations of IS and PCS were both 50 μg / ml.

[0046] Danhong injection sample preparation: Take 165 μl of human mixed plasma sample, add 10 μl of IS aqueous solution and 5 μl of PCS aqueous solution, vortex for 30 s to mix well, then let stand at room temperature...

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Abstract

The invention discloses application of danshinolic acid components in preparing pharmaceutical preparations for improving the dialysis rate and filtration rate of protein-bound uremic toxins. The danshinolic acid components include danshinolic acid B, protocatechuic aldehyde, rosmarinic acid, other danshinolic acid monomer components, pharmaceutical preparations containing salviae miltiorrhizae, pharmaceutical preparations containing salviae miltiorrhizae and safflower, and medicine suits with different salviae miltiorrhizae and safflower compatibility proportions. Through screening of a lot of experiments, results show that salviae miltiorrhizae and safflower injections and the danshinolic acid components can remarkably improve the dialysis efficiency of the protein-bound uremic toxins, can remarkably improve the filtering efficiency of the protein-bound uremic toxins, can be developed into auxiliary medicine preparations of in-vitro kidney replacement therapy strategies such as kidney disease dialysis and blood filtering, and have good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicines, and specifically relates to the application of salvianolic acid components in the preparation of preparations for improving the dialysis rate and filtration rate of protein-bound uremic toxins. Background technique [0002] The renal function of patients with end-stage renal disease is severely impaired, and the metabolic waste in the body cannot be effectively removed, resulting in the accumulation of more than 90 kinds of uremic toxins in the patient's body. Accumulated uremic toxins can induce complications such as bone disease, encephalopathy, cardiovascular disease, anemia, soft tissue calcification, and abnormal immune response. Reducing the level of uremic toxins in the patient's body can significantly improve the treatment outcome of patients with kidney disease. Uretoxins mainly include small molecule uretoxins (molecular weight <500Da), protein-bound small molecule uretoxins (mole...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61K31/216A61K31/192A61P13/12
CPCA61K31/192A61K31/216A61K31/343A61P13/12A61K2300/00
Inventor 李建萍郭建明段金廒
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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