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Method for extracting polypeptide from animal kidney

A technology of animal kidney and extraction method, applied in the direction of fermentation, etc., to achieve the effect of good effect, high degreasing rate and improved quality

Inactive Publication Date: 2018-09-07
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, reports on extracting active peptides from animal kidneys using enzymatic hydrolysis technology are relatively rare.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for extracting animal kidney polypeptides. The animal kidneys are degreased with an aqueous solution containing sodium carbonate and ethyl valerate, and then enzymatically hydrolyzed by a compound protease. During the enzymatic hydrolysis, ascorbic acid is added, and the obtained enzymatic hydrolyzate is ultrafiltered, Gel column chromatography to obtain the kidney polypeptide. The extraction method is simple and feasible, and can effectively enzymatically hydrolyze macromolecular proteins. The obtained enzymolysis solution has low oligopeptide content and high polypeptide content, and has the advantages of low investment, simple operation, good protein degradation effect, and high kidney polypeptide yield. The advantages.

[0015] The specific steps of raw material pretreatment are: wash the animal kidney, drain it, chop it up and then mash it, add 3% sodium carbonate and 0.031% valeric acid according to the material-liquid ratio of 1:8 (g / mL) The aqueous sol...

Embodiment 2

[0020] A method for extracting animal kidney polypeptides, the specific steps of which are:

[0021] 1) Raw material pretreatment: wash the animal kidneys, drain them, chop them up and then mash them, add 2% sodium carbonate and 0.035% ethyl valerate according to the ratio of solid to liquid 1:10 (g / mL) Degreasing the aqueous solution at a temperature of 40°C and an ultrasonic frequency of 45kHz for 25 minutes, discarding the lye, washing with water until nearly neutral, and drying to obtain defatted kidney powder;

[0022] 2) Enzymolysis: Add deionized water to the defatted kidney powder at a substrate concentration of 1.5%, adjust the pH to 8.5, add a compound protease at a 5.0% enzyme amount, and the weight ratio of Alcalase and flavor protease in the compound protease is 0.6:1, then microwave enzymolysis at a temperature of 50°C and a microwave power of 160W for 2 hours, then add 0.35% ascorbic acid by weight of flavor protease, and continue enzymolysis for 60 minutes. Af...

Embodiment 3

[0026] A method for extracting animal kidney polypeptides, the specific steps of which are:

[0027] 1) Raw material pretreatment: wash the animal kidneys, drain them, chop them up and then mash them, add 2% sodium carbonate and 0.035% ethyl valerate according to the ratio of solid to liquid 1:10 (g / mL) Degreasing the aqueous solution at a temperature of 40°C and an ultrasonic frequency of 45kHz for 25 minutes, discarding the lye, washing with water until nearly neutral, and drying to obtain defatted kidney powder;

[0028] 2) Enzymolysis: Add deionized water to the defatted kidney powder at a substrate concentration of 1.5%, adjust the pH to 8.5, add a compound protease at a 5.0% enzyme amount, and the weight ratio of Alcalase and flavor protease in the compound protease is 0.6:1, then microwave enzymolysis at a temperature of 50°C and a microwave power of 160W for 2 hours, then add 0.35% ascorbic acid by weight of flavor protease, and continue enzymolysis for 60 minutes. Af...

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PUM

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Abstract

The invention discloses a method for extracting polypeptide from animal kidney. The method comprises steps as follows: the animal kidney is subjected to degrease treatment with an aqueous solution containing sodium carbonate and ethyl valerate and then is subjected to enzymolysis by compound protease, ascorbic acid is added in an enzymolysis process, an obtained enzymatic hydrolysate is subjectedto ultrafiltration and gel column chromatography treatment, and the kidney polypeptide is obtained. The method for extracting polypeptide from the animal kidney is simple and practicable and has the advantages of low investment, simple operation, good protein degradation effect and high kidney polypeptide yield, macromolecular protein can be subjected to enzymolysis effectively, and the obtained enzymatic hydrolysate is low in oligopeptide content and high in polypeptide content.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting animal kidney polypeptides. Background technique [0002] Active peptides, also known as functional peptides, are some special fragment sequences contained in proteins. They are peptide compounds that are beneficial to the life activity of organisms or have physiological effects. They are composed of 20 natural amino acids in proteins with different compositions and arrangements. A general term for different peptides ranging from dipeptides to complex linear and cyclic structures, and is also a multifunctional compound derived from proteins. Fundamentally, it has a positive effect on human health, and it can affect the main systems of the body - cardiovascular system, digestive system, immune system and nervous system after oral administration. It is a class of functional factors with special functions such as scavenging free radicals, impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
CPCC12P21/06C07K1/16C07K1/34C07K1/36
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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