Preparation method of S-ABA

Abstract

The invention discloses a preparation method of S-ABA. The preparation method comprises the following steps: (1) with a strain capable of generating S-ABA through fermentation, carrying out seed culture and fermentation culture to obtain fermentation broth containing S-ABA; (2) carrying out plate and frame filtration, membrane filtration, acid precipitation, and extraction on the fermentation broth containing S-ABA obtained in the step (1) to obtain an extract; and (3) carrying out concentrated crystallization, recrystallization and drying on the extract obtained in the step (2) to obtain theS-ABA product with purity of 95% or more. The preparation method can shorten the fermentation cycle, increase the content of S-ABA generated by fermentation, avoid the waste of S-ABA by extracting andrefining treatment, and improve the purity of S-ABA, so that the purity of S-ABA in the final product is 95% or higher, and the product quality is enhanced. In addition, the preparation process is mild, and the preparation process is environmentally friendly, which is beneficial to the industrial production of S-ABA.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on April 18, 2017, the application number is 201710254199.4, and the invention title is "a method for preparing S-induced antibiotics", the entire content of which is incorporated by reference In this application. technical field [0002] The invention relates to a preparation method of S-induced antigen. Background technique [0003] S-abscisic acid (natural abscisic acid), together with auxin, ethylene, gibberellin, and cytokinin, is listed as the five natural growth regulators of plants. Reverse gene, induces and activates the anti-stress immune system in plants, improves the plant's own resistance to cold, drought, pests, and salinity, and can reduce the use of chemical pesticides by applying S-absorbin, which can improve the quality of agricultural products, etc. It has important physiological activity and application value in many aspects. [0004] At pr...

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Problems solved by technology

The direct extraction method refers to direct extraction from plants, but due to the low content in the plant body, the production method has a low yield and a small scale; the chemical synthesis method prepares S-antibodies, and its synthetic purity is low, because The products obtained by chemical synthesis are non-natural abscisic acid and natural abscisic acid (S-abscisic acid), only natural abscisic acid (S-abscisic acid) has the above physiological activities, and the production cost of chemical synthesis is extremely high, so the current Only Japan, the United States and other developed countries are used in large-scale agricultural production; the S-abscisin produced by the biological fermentation method has a high yield and will not produce unnatural abscisic acid. However, there are still some technical problems in this method. For example, the existing fermentation process has a long period, the content of S-absorbin produced needs to be improved, and there are many by-products produced by fermentation, which cause certain difficulties in extraction. , and the extracted process parameters are not easy to control, resulting in a large amount of waste of S-induced antibiotics. In addition, because of the defects of the process and the control of the process parameters, the purity of the final S-induced antibiotics product is not high, only less than 95%. Quality cannot be improved

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