Endophytic fungi 2cpe-1 of kandelia candel of mangrove plants, fermenting liquid and application thereof

A 2cpe-1, mangrove plant technology, applied in the field of microorganisms, to achieve the effect of wide application prospects

Active Publication Date: 2018-09-18
广东立威化工有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic and foreign research reports on the antibacterial activity of endophytic fungi of mangrove plant candela on plant pathogens

Method used

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  • Endophytic fungi 2cpe-1 of kandelia candel of mangrove plants, fermenting liquid and application thereof
  • Endophytic fungi 2cpe-1 of kandelia candel of mangrove plants, fermenting liquid and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A mangrove plant candela endophytic fungus 2cpe-1 provided by the present invention has a preservation number of CGMCC No.15388; on February 07, 2018, it was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee. Preservation address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Classification named Penicillium (Penicillium sp.).

[0030] It is isolated from the leaves of candela japonica in Hongguang Village Mangrove Reserve, Yangjiang, Guangdong Province, China.

[0031] The specific separation method is as follows:

[0032] The collected mangrove plant candelabra had no wormholes and withered leaf samples were rinsed with tap water several times to remove the surface soil, then washed 3 times with sterile water, and sterilized the surface with 75% ethanol, and the fresh leaves were washed successively with sodium hypochlorite (containi...

Embodiment 2

[0035] A kind of mangrove plant candela endophytic fungus 2cpe-1 fermented liquid provided by the invention is specifically prepared by the following steps:

[0036] (1) Activation of bacteria

[0037] Under sterile conditions, use an inoculation loop to pick a small amount of preserved endophytic fungus strains of mangroves, transfer them to sterilized potato dextrose agar solid medium plates, and cultivate them at a constant temperature for 5 days at a temperature of 28 ° C ± 1 ℃, to obtain activated strains;

[0038] Each component and its content in the described potato dextrose agar solid medium are: 200 grams of potatoes, 20 grams of glucose, 20 grams of agar, 1000 mL of artificial seawater; (artificial seawater is 30 g of crude sea salt dissolved in 1000 mL of water)

[0039] (2) Seed cultivation

[0040] Under sterile conditions, use an inoculation loop to pick 3 rings of activated strains, inoculate them in 50ml of sterilized potato glucose liquid medium, and cultiv...

Embodiment 3

[0048] The antibacterial test of the mangrove plant candela endophyte (Penicillium sp.) 2cpe-1 fermented liquid provided by the present invention is as follows.

[0049] 1. Plant pathogenic fungi:

[0050] Phytopathogenic fungi Fusarium graminearum, Phytophthora sojae and Colletotrichum musae

[0051] 2. The cultivation of pathogenic microorganisms

[0052] Use an inoculation loop to pick up a small amount of slant cultures of pathogenic bacteria and insert them into potato dextrose agar solid medium plates for activation, and cultivate them in an incubator at (28±1)°C for 5 days.

[0053] 3. Determination of antibacterial activity by inhibiting mycelium growth rate method

[0054] In a sterile Erlenmeyer flask of 100 milliliters, prepare the aseptic mixed culture medium (final concentration is 250mg / L and 500mg / L) of methanol eluate (final concentration is 250mg / L and 500mg / L) after the methanol eluate that contains fermented liquid, and the mycelia methanol extract contain...

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Abstract

The invention provides endophytic fungi 2cpe-1 of kandelia candel of mangrove plants, fermenting liquid and application thereof. With the collection number of CGMCC No. 15388, the endophytic fungi 2cpe-1 of the kandelia candel of the mangrove plants is collected at China General Microbiological Culture Collection Center on the 7th February, 2018. The invention aims at providing the endophytic fungi 2cpe-1 of the kandelia candel of the mangrove plants and the fermenting liquid thereof. The endophytic fungi 2cpe-1 is a strain with more obvious inhibiting effects for the growth of hyphae of plantpathogenic fungi such as fusarium graminearum, phytophthora sojae and colletotrichum musae, can used as a novel agricultural antibiotic natural active substance for resisting the diseases of the plant pathogenic fungi, and belongs to the technical field of microorganisms.

Description

technical field [0001] The invention discloses a tree plant candela endophyte 2cpe-1, and also discloses a fermented liquid and an application of the tree plant candela endophyte 2cpe-1, belonging to the technical field of microorganisms. Background technique [0002] Plant pathogenic bacteria are mainly controlled by chemical control. Due to the long-term application and improper use of chemical pesticides, the phenomenon of increased resistance of harmful organisms, continuous emergence of new diseases, increasingly serious environmental pollution, and serious excessive pesticide residues have seriously threatened human health and The living environment urgently needs to develop new pesticides. [0003] Candelia (Kandelia candel) is a plant belonging to the family Rhizophoraceae (Rhizophoraceae), which is a halophytic plant that grows in shallow water swamps from the upper part of the tropical and subtropical coastal intertidal zone to the subtidal zone. Folks have a long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P3/00C12R1/80
CPCA01N63/30C12N1/14C12P1/02C12N1/145C12R2001/80
Inventor 叶炎婵董钢黄华容方岩雄
Owner 广东立威化工有限公司
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